“assembly”的搜索结果

Assembles microbial-community sequencing into metagenome-assembled genomes (MAGs) with metaFlye (ONT), metaSPAdes/MEGAHIT (Illumina), and hifiasm-meta/metaMDBG (PacBio HiFi), then recovers genomes via multi-binner consolidation (MetaBAT2, MaxBin2, CONCOCT, SemiBin2, VAMB -> DAS_Tool) and QCs them against MIMAG with CheckM2, GUNC, and GTDB-Tk. Covers why a metagenome is not a genome (uneven coverage, micro-diversity, strain collapse to consensus), differential-coverage binning, co-assembly vs per-sample, the rRNA-operon collapse that fails short-read MAGs, and strain resolution with inStrain. Use when reconstructing genomes from a microbiome, soil, ocean, or gut community, recovering MAGs, or resolving strain-level variation.

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Detects and removes contamination in genome assemblies via two disjoint workflows - foreign-sequence screening of a single-organism (eukaryote/isolate) assembly with NCBI FCS-GX (GenBank-submission-mandatory), FCS-adaptor, and BlobToolKit blob plots; and MAG/bin quality assessment with CheckM2 plus GUNC (chimerism) plus GTDB-Tk taxonomy, judged against MIMAG. Covers why CheckM2 alone is blind to disjoint-marker chimeras, the FCS-GX RAM wall, organelle/NUMT triage, strain heterogeneity, and the HGT-vs-contamination (tardigrade) trap. Use when screening an assembly for foreign contamination before GenBank submission, assessing MAG completeness/contamination/chimerism, deciding which contigs to remove, or distinguishing real HGT from contaminant contigs.

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Assembles a genome de novo from Illumina short reads with SPAdes (isolate/careful/sc/meta/plasmid/rna modes), MEGAHIT (low-memory, huge datasets), Unicycler (bacterial finishing/hybrid), MaSuRCA (large hybrid), ABySS (Bloom-filter), and Platanus (heterozygous diploids), using multi-k de Bruijn graphs. Covers the repeat-resolution limit, why N50 plateaus at the genome not the depth, GenomeScope2 k-mer profiling first, the heterozygosity/haplotig trap, error-correction erasing rare alleles, GC dropout, and NG50/auN/BUSCO reporting. Use when assembling a bacterial isolate, fungal, small-eukaryotic, single-cell, or metagenome genome from Illumina reads, or when deciding whether short reads can even produce the assembly being asked for.

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Select, document, download, and checksum-verify the most appropriate NCBI reference genome on an HPC cluster. Use whenever an analysis needs a reference genome, genome FASTA, genome annotation, assembly accession, or genome index and no exact approved assembly is already available; when a user asks to fetch a species genome from NCBI; or when a pipeline must resolve and cache a reproducible reference assembly on a remote cluster.

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Convert genomic positions between file conventions, standardize variant representations, and check reference genome or chromosome naming conflicts. The skill helps investigate one-base shifts and compare individual variants against a reference sequence before combining analysis files.

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• Converting coordinates between BED and GFF/GTF
• Standardizing and comparing insertion or deletion records
• Checking reference genome and chromosome naming conflicts

Inspect executable files from the command line with radare2, starting with file details, strings, and imported functions. Trace code through disassembly, compare binaries, automate inspections, or apply explicitly requested patches with verification.

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• Inspecting an unfamiliar executable file
• Finding the function that uses an error message
• Comparing two versions of a binary

Analyze compiled .NET and C# programs by identifying managed files, removing obfuscation, inspecting intermediate instructions, and debugging runtime behavior. Save extracted configuration and code changes as evidence for further review.

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• Inspect compiled .NET program behavior
• Analyze obfuscated C# programs
• Extract malware configuration and communication destinations

Prepare an initial equity research report through five separate tasks covering company research, financial forecasts, valuation, charts, and report assembly. Outputs include research notes, an Excel financial model, chart files, and a detailed Word report.

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• Researching a company’s business, competitors, and risks
• Building financial forecasts with three scenarios
• Estimating a price target using multiple valuation methods

Transcribe audio files through 9Router using models from providers such as OpenAI, Groq, and Deepgram. Receive plain text, structured results with timestamps, or SRT and VTT subtitles, depending on the selected model’s support.

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• Transcribing recorded meetings or interviews
• Creating SRT or VTT subtitles from audio
• Getting transcriptions with timestamps

Create static technical drawings from object descriptions, photographs, or CAD models. Produce consistent views that explain shape or assembly, plus a viewer with zoom, editable colors, and downloads in SVG, PNG, and PDF formats.

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• Creating an exploded view to explain an assembly
• Drawing aligned views from supplied dimensions
• Illustrating an object's shape from photographs

Add burned-in captions to a finished video using Whisper timings from its own audio. Choose paper, bold, or clean styling, and optionally use narration text to preserve intended spelling. Outputs include the captioned video and an SRT file.

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• Adding captions to a finished video
• Restyling captions for social media shorts
• Preserving brand names and numbers from a narration script

Build visual sequences that move forward and backward with page scrolling. Use video, image frames, canvas, SVG, or HTML elements in a sticky stage, while preserving keyboard access, static alternatives, and predictable visual states.

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• Showing product assembly through scrolling
• Explaining interface states or diagrams step by step
• Rotating objects or advancing photo sequences with scrolling

Build a scanning entrance for Three.js models, revealing their wire structure before the solid surface appears. Coordinate both layers with one expanding wave, then remove the temporary wires when the animation finishes.

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• Introduce a 3D model with a radial scan
• Show a model's structure before its surface
• Create a holographic assembly effect

Fix Blazor issues during prerendering, when the server sends initial HTML before interactive features load. Preserve component state to avoid repeated data requests and flicker, handle unavailable services, and configure prerendering or page routing where needed.

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• Fixing duplicate data requests on initial page loads
• Reducing flicker when a page becomes interactive
• Handling client services unavailable during prerendering

Share shopping carts, themes, and other state across Blazor components that are not directly connected. Choose cascading values or shared services, notify components when data changes, and account for rendering boundaries and service lifetimes.

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• Sharing a shopping cart across multiple pages
• Applying themes or preferences throughout an app
• Updating notification counts in separate components

The reference agents' cache-stable request assembly, covering the static system and per-request context split, the fixed tool list, the rolling conversation breakpoint, which config fields are prompt bytes, and verification. Load when writing or reviewing a commerce agent's prompt assembly, when cache reads are zero, or when a turn's latency or cost is the question.

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JLCPCB PCB fabrication and assembly — BOM/CPL generation, basic vs extended parts, assembly constraints, design rules, ordering workflow. Use with KiCad for JLCPCB manufacturing. Use this skill when the user mentions JLCPCB, wants to order PCBs or assembled boards, needs prototype bare PCBs and stencils, wants to know JLCPCB design rules and capabilities, or is asking about PCB manufacturing costs or turnaround times. For gerber/CPL export, stencil ordering, and BOM management, see the `bom` skill.

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Search LCSC Electronics for electronic components — find parts by LCSC number (Cxxxxx) or MPN, check stock/pricing, download datasheets, analyze specifications. Sister company to JLCPCB, same parts library. Sync and maintain a local datasheets directory for a KiCad project, or use batch MPN-list seeding (`--mpn-list`) for bulk workflows without a project. No API key needed — uses the free jlcsearch community API. Use this skill when the user mentions LCSC, JLCPCB parts library, JLCPCB assembly parts, production sourcing, Cxxxxx part numbers, needs to find LCSC equivalents for parts, is preparing a BOM for JLCPCB assembly, or wants to download datasheets and LCSC is available. For package cross-reference tables and BOM workflow, see the `bom` skill.

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JLCPCB PCB fabrication and assembly — BOM/CPL generation, basic vs extended parts, assembly constraints, design rules, ordering workflow. Use with KiCad for JLCPCB manufacturing. Use this skill when the user mentions JLCPCB, wants to order PCBs or assembled boards, needs prototype bare PCBs and stencils, wants to know JLCPCB design rules and capabilities, or is asking about PCB manufacturing costs or turnaround times. For gerber/CPL export, stencil ordering, and BOM management, see the `bom` skill.

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Search LCSC Electronics for electronic components — find parts by LCSC number (Cxxxxx) or MPN, check stock/pricing, download datasheets, analyze specifications. Sister company to JLCPCB, same parts library. Sync and maintain a local datasheets directory for a KiCad project, or use batch MPN-list seeding (`--mpn-list`) for bulk workflows without a project. No API key needed — uses the free jlcsearch community API. Use this skill when the user mentions LCSC, JLCPCB parts library, JLCPCB assembly parts, production sourcing, Cxxxxx part numbers, needs to find LCSC equivalents for parts, is preparing a BOM for JLCPCB assembly, or wants to download datasheets and LCSC is available. For package cross-reference tables and BOM workflow, see the `bom` skill.

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Assemble a creator picture-in-picture product-listicle ad from a config — the creator stays FULL-FRAME the whole beat (voice plus lips generated together per beat, no separate VO, no cut to a full-frame product shot), and on each product beat three persistent overlays ride on top for the WHOLE beat — a title pill top-center, the DEMO in a rounded PiP window top-right (the brand's real UGC clip MUTED, or for a no-UGC brand the product's own autocropped UI still / screen-recording sized to fill the window), and a bottom product card (rounded thumbnail plus 'N · CATEGORY' small-caps plus product NAME in a serif face). Hook plus CTA beats are the creator full-frame with the title pill only. Assembly builds ONE full-1080x1920 transparent overlay PNG per beat, overlays it on the creator clip (cover-scaled to 1080x1920) for the whole beat keeping the native audio, concats all beats, then burns captions LAST as timed PIL PNG overlays (this ffmpeg has no libass) timed deterministically from the known per-beat script. This is the FREE deterministic assembly stage (overlay-PNG build plus cover-scale composite plus concat plus PIL-PNG caption burn); the creator anchor and the N native talking clips come from create-image-fal (Seedream v5 Pro) and create-video-fal (Seedance 2.0). Use for the creator-pip-listicle format.

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Orders and orients assembled contigs into chromosome-scale scaffolds from long-range linking data, inserting N-gap spacers (adds no sequence). Covers Hi-C/Omni-C scaffolding (YaHS, SALSA2, 3D-DNA/Juicer), Hi-C read-mapping prerequisites (map each end separately, no mate rescue, dedup, enzyme-aware), reading the contact map for misjoins/inversions/false-duplications, manual curation in Juicebox/PretextView (the VGP/DToL standard), reference-guided scaffolding (RagTag) and its karyotype-erasure hazard, genetic-map (ALLMAPS) and Bionano optical-map integration, chimera-breaking before scaffolding, gap-filling, and telomere/contig-vs-scaffold-N50 QC (tidk). Use when turning contigs into chromosomes with Hi-C, integrating a linkage map or optical map, choosing a scaffolder by available linking data, or judging whether a chromosome-scale assembly is trustworthy.

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.NET / C# 二进制逆向。当目标是 .NET assembly（PE 头含 CLR、.exe/.dll 托管程序）、C# 编译产物（含 NativeAOT）、红队 Sharp* 工具（Rubeus / SharpHound / SharpHound 等）、.NET 混淆程序（ConfuserEx / SmartAssembly / Babel / Eazfuscator）、.NET loader / info-stealer / 套壳 malware 时使用。优先用 dnSpyEx + de4dot，需要 AI 直接操作时联动 dnSpy MCP。不用于纯 native 二进制（走 reverse-engineering / ida-reverse）。

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Build cross-platform .NET applications with Uno Platform targeting WebAssembly, iOS, Android, macOS, Linux, and Windows from a single XAML/C# codebase. USE FOR: building cross-platform apps from a single C# and XAML codebase; targeting WebAssembly, iOS, Android, macOS, Linux, and Windows simultaneously; migrating WPF or UWP. DO NOT USE FOR: unrelated stacks; generic tasks that do not need this specific guidance. INVOKES: inspect the repository context, edit targeted files, and run relevant build, test, lint, or validation commands when changes are made.

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