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Use when working with Aws S3 — aWS S3 bucket analysis, storage class distribution, lifecycle policies, access patterns, and cost optimization. Covers bucket inventory, object metrics, versioning status, encryption audit, public access analysis, and intelligent tiering evaluation.
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S3, multipart uploads, CDN integration, and presigned URLs for file storage. Activate on: file upload, S3, presigned URL, multipart upload, CDN, blob storage, image upload, media storage. NOT for: data warehouse storage (use lakehouse-architect), database design (use dimensional-modeler).
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Manage files and storage buckets in Tigris through its official CLI. Agents can upload and download objects, generate temporary sharing URLs, take snapshots, and manage access keys and permissions with structured JSON output.
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Review AWS spending and identify savings through resource sizing, unused resource cleanup, discount commitments, and storage policies. It also guides budget alerts and recurring cost reviews so teams can track spending and plan changes using usage data.
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Prepare an M5Stack device connected over USB by detecting it, flashing UIFlow 2.0 firmware, and installing MicroPython apps such as Claude Buddy. Also refresh installed apps or check the display, speaker, buttons, and connected components.
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Set up and operate Amazon GuardDuty to detect threats in AWS. Configure protection features, centralize findings across accounts, investigate suspicious activity, and build automated responses with EventBridge and Lambda, including containment of potentially compromised resources.
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Prepare SageMaker deployments and training by finding existing execution roles, checking trust settings, and reviewing required permissions. Guides role creation only when necessary and identifies permission issues that require an AWS administrator.
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Investigate subdomain takeover risks caused by DNS records pointing to abandoned services. The skill covers discovery, provider error checks, resource claimability, and evidence collection, then examines potential effects on login flows, shared cookies, and trusted content.
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Guide authorized subdomain takeover assessments involving DNS records that point to unclaimed resources. The skill defines boundaries, suggests further checks, and specifies evidence for takeover feasibility, potential impact, or a report of unsuccessful investigation.
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Provisions, connects, migrates, and operates Amazon RDS for Db2. Applies when provisioning with IBM customer and site IDs (License Manager, BYOL, GovCloud), connecting over TLS, fixing SQL30082N after Secrets Manager rotation, migration from Db2 LUW (Linux, AIX, Windows, AS400) or z/OS mainframe (ADB2GEN, Q Replication), choosing code page/collation (EBCDIC, CCSID), S3 backup/restore, Multi-AZ and cross-region standby replicas, RDSADMIN procedures, customer-managed KMS BYOK, self-managed Active Directory Kerberos, Db2 audit to S3, minimum IAM, or colocation.
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Execute and manage Athena SQL queries across default and federated catalogs (Glue, S3 Tables, Redshift). Triggers on phrases like: query data, run SQL, athena query, analyze table, SQL query, workgroup status, profile table, query Redshift catalog, query S3 Tables. Do NOT use for finding specific data assets (use finding-data-lake-assets), full catalog audits (use exploring-data-catalog), importing data (use ingesting-into-data-lake).
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Calls m6A peaks from MeRIP-seq / m6A-seq paired IP-vs-input data using exomePeak2 (transcript-aware, GC-bias-corrected Poisson GLM), MeTPeak (HMM over sliding windows), MACS3/MACS2 with --nomodel --broad --keep-dup all (genome-wide broad alternative), and DRACH motif enrichment via HOMER or ggseqlogo as a sanity check (NOT a filter). Covers BED12 vs narrowPeak output, exonic vs intronic peak handling, multi-tool reconciliation (intersection vs union), the m6A-vs-m6Am ambiguity at 5'UTR peaks that antibody methods cannot resolve, and orthogonal validation (miCLIP/GLORI/m6A-SAC-seq/m6Anet). Use when calling peaks from paired IP/input genome BAMs, choosing exomePeak2 (transcript-aware default) vs MACS3 (broad genomic) vs MeTPeak (HMM-smoothed low-coverage), confirming DRACH enrichment as a sanity check on the peak set, reconciling differing peak sets across tools, validating MeRIP peaks against single-base methods, interpreting 5' peaks where m6Am contamination is possible, or recommending a consensus strategy.
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Processes eDNA metabarcoding from raw paired-end reads to species tables, navigating ASV (DADA2, UNOISE3) vs OTU (swarm v2) decision (Callahan 2017 vs Schloss multi-copy-16S critique), marker/primer choice (Leray COI, MiFish 12S, 515F/806R 16S, ITS2) with primer-specific bias, OBITools3 v3 command-name break (obi stats plural; .tar.gz taxonomy), tag-jumping with dual-indexing (Schnell 2015; NovaSeq 10x MiSeq), decontam as screening-not-classifier (Davis 2018), read-counts-not-abundance critique (Lamb 2019), site-occupancy modeling (Ficetola 2015), Naive-Bayes calibration limits (Bokulich 2018), and eDNA decay (Strickler 2015). Use when going from raw eDNA FASTQ to species tables, picking marker + denoising pipeline, deciding whether read counts represent abundance, applying occupancy modeling, configuring OBITools3 v3, or interpreting decontam output. Not for clinical 16S microbiome (see microbiome/amplicon-processing).
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Applies ACMG/AMP 2015 framework with ClinGen SVI specifications, Tavtigian 2018/2020 Bayesian point system, Abou Tayoun 2018 PVS1 decision tree, Pejaver 2022 and Bergquist 2025 calibrated PP3/BP4 thresholds for REVEL/BayesDel/AlphaMissense, Brnich 2020 PS3/BS3 OddsPath, Walker 2023 SpliceAI splicing framework, and AMP/ASCO/CAP 2017 tumor tiers. Use when classifying germline variants P / LP / VUS / LB / B, applying VCEP-specific CSpec rules, computing Whiffin BS1, or assigning cancer Tier I-IV per Li 2017.
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Configure S3 buckets, policies, and lifecycle rules. Implement versioning, replication, and security. Use when managing object storage on AWS.
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AWS S3 object storage for bucket management, object operations, and access control. Use when creating buckets, uploading files, configuring lifecycle policies, setting up static websites, managing permissions, or implementing cross-region replication.
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Validate and stream file uploads securely with Validation and S3 streaming in NestJS. Use when implementing secure file uploads, validation, or S3 streaming in NestJS.
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Poisson-model peak caller for ChIP-seq/ATAC-seq BAMs. MACS3 callpeak finds enriched regions (TF sites or histone marks) vs input/IgG; outputs BED narrowPeak/broadPeak for motif analysis, annotation, and differential binding. Use narrow peaks for TF ChIP-seq and ATAC-seq; broad for H3K27me3, H3K9me3, and other broad marks.
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Configure S3 buckets, policies, and lifecycle rules. Implement versioning, replication, and security. Use when managing object storage on AWS.
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Add custom metadata headers (x-amz-meta-*) to AWS v4 signed requests for GCS S3-compatible API. Use when: (1) Adding custom metadata to GCS uploads via S3 API, (2) Getting signature mismatch errors after adding new headers, (3) x-amz-meta-* headers being ignored or causing 403 errors. Custom headers MUST be included in canonical headers and signed headers list.
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Use Amazon Transcribe for AWS-based speech-to-text production: batch S3 transcription, real-time streaming, captions/subtitles, diarization, channel identification, custom vocabularies, vocabulary filters, language identification, PII/PHI handling, toxicity detection, Call Analytics, Medical, and secure S3/IAM/KMS workflows.
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Applies ACMG/AMP 2015 framework with ClinGen SVI specifications, Tavtigian 2018/2020 Bayesian point system, Abou Tayoun 2018 PVS1 decision tree, Pejaver 2022 calibrated PP3/BP4 thresholds for REVEL/BayesDel/AlphaMissense, Brnich 2020 PS3/BS3 OddsPath, Walker 2023 SpliceAI splicing framework, and AMP/ASCO/CAP 2017 tumor tiers. Use when classifying germline variants P / LP / VUS / LB / B, applying VCEP-specific CSpec rules, computing Whiffin BS1, or assigning cancer Tier I-IV per Li 2017.
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The AWS Command Line Interface (v2) manages Amazon Web Services from the terminal. Use when the user needs to run S3, EC2, Lambda, CloudWatch, IAM or other AWS commands, sign in with SSO or short-term credentials, write AWS automation scripts, or debug an `aws` command and its output.
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Expert guide for file uploads (S3, R2, Supabase Storage), presigned URLs, image/video processing, CDN optimization, and media pipeline architecture / Panduan ahli untuk upload file (S3, R2, Supabase Storage), presigned URL, pemrosesan gambar/video, optimasi CDN, dan arsitektur pipeline media.
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