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Prepare single-cell or single-nucleus RNA-seq analysis by checking inputs and selecting workflows that turn FASTQ sequencing files into count matrices. Route quality checks, cell annotation, clustering, and UMAP visualization to the embedded downstream skill.
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Build or review quality control workflows for single-cell and single-nucleus RNA sequencing data. Select filters from observed data, detect doublets, assign cell types, and create UMAP plots that summarize cell relationships, while recording analysis decisions and outputs.
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Add reveal animations that start when page elements enter the visible area. The skill provides JavaScript using IntersectionObserver, a browser visibility detector, plus CSS keyframes and Tailwind-compatible classes, with adjustments for trigger position, motion, duration, and delay.
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Query the Genome Aggregation Database (gnomAD). Use when determining the rarity or allele frequency of specific genetic variants, retrieving gene constraint metrics (pLI, LOEUF) to assess loss-of-function intolerance, finding variants in a genomic region or gene, or querying structural variants. Don't use for analyzing individual patient genomes, tracking somatic mutations in cancer (use COSMIC), or requesting raw sequencing reads (use ENA).
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Apply when sequencing an addition, refactor, or rewrite. Remove dead code, redundant validators, and stub references first, then build on the simpler base.
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Apply before writing logic: choosing core types and data structures, sequencing scaffold-vs-feature work, asking what concurrent actors share. Get the data structures right so downstream code becomes obvious.
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Generate and interpret quality reports from FASTQ files using FastQC and MultiQC. Assess per-base quality, adapter content, GC bias, duplication levels, and overrepresented sequences. Use when performing initial QC on raw sequencing data or validating preprocessing results.
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Extract, process, and deduplicate reads using Unique Molecular Identifiers (UMIs) with umi_tools. Use when library prep includes UMIs and accurate molecule counting is needed, such as in single-cell RNA-seq, low-input RNA-seq, or targeted sequencing to distinguish PCR from biological duplicates.
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Remove sequencing adapters from FASTQ files using Cutadapt and Trimmomatic. Supports single-end and paired-end reads, Illumina TruSeq, Nextera, and custom adapter sequences. Use when FastQC shows adapter contamination or before alignment of short reads.
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Extract methylation calls from Bismark BAM files using bismark_methylation_extractor. Generates per-cytosine reports for CpG, CHG, and CHH contexts. Use when extracting methylation levels from aligned bisulfite sequencing data for downstream analysis.
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Identify tumor neoantigens from somatic mutations using pVACtools for personalized cancer immunotherapy. Predict mutant peptides that bind patient HLA and may elicit T-cell responses. Use when identifying vaccine targets or checkpoint inhibitor response biomarkers from tumor sequencing data.
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Deep learning-based variant calling from long reads using Clair3 for SNPs and small indels. Use when calling germline variants from ONT or PacBio alignments, particularly when high accuracy is needed for clinical or research applications.
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Predict metagenome functional content from 16S rRNA marker gene data using PICRUSt2. Infer KEGG, MetaCyc, and EC abundances from ASV tables. Use when functional profiling is needed from 16S data without shotgun metagenomics sequencing.
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Quality control for long-read sequencing data using NanoPlot, NanoStat, and chopper. Generate QC reports, filter reads by length and quality, and visualize read characteristics. Use when assessing ONT or PacBio run quality or filtering reads before assembly or alignment.
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Amplicon sequence variant (ASV) inference from 16S rRNA or ITS amplicon sequencing using DADA2. Covers quality filtering, error learning, denoising, and chimera removal. Use when processing demultiplexed amplicon FASTQ files to generate an ASV table for downstream analysis.
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Analyzes cfDNA methylation patterns for cancer detection using cfMeDIP-seq or bisulfite sequencing with MethylDackel. Identifies cancer-specific methylation signatures and performs tissue-of-origin deconvolution. Use when using methylation biomarkers for early cancer detection or minimal residual disease.
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Analyze PacBio Iso-Seq data for full-length isoform discovery and quantification. Use when characterizing transcript diversity or identifying novel splice variants.
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Process Hi-C read pairs using pairtools. Parse alignments, filter duplicates, classify pairs, and generate contact statistics from Hi-C sequencing data. Use when processing raw Hi-C read pairs.
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Filter and prioritize variants by pathogenicity, population frequency, and clinical evidence for rare disease analysis. Use when identifying candidate disease-causing variants from exome or genome sequencing.
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CRISPResso2 for analyzing CRISPR gene editing outcomes. Quantifies indels, HDR efficiency, and generates comprehensive editing reports. Use when analyzing amplicon sequencing data from CRISPR editing experiments to assess editing efficiency.
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Start and scale networked products using Andrew Chen's "The Cold Start Problem" framework for network effects. Use when the user mentions "network effects", "chicken and egg", "cold start", "two-sided marketplace", "atomic network", "hard side", "liquidity", "critical mass", "invite-only launch", "how do I get my first users", or "the marketplace has no buyers or sellers". Also trigger when launching a marketplace, social, or collaboration product that is worthless without other users, deciding launch sequencing and seeding tactics, or diagnosing stalled network growth at scale. Covers the five stages: cold start, tipping point, escape velocity, hitting the ceiling, and the moat. For word-of-mouth virality, see contagious. For habit-driven retention, see hooked-ux.
中文简介正在准备中。当前显示原文说明。
Apply when sequencing an addition, refactor, or rewrite. Remove dead code, redundant validators, and stub references first, then build on the simpler base.
中文简介正在准备中。当前显示原文说明。
Apply before writing logic: choosing core types and data structures, sequencing scaffold-vs-feature work, asking what concurrent actors share. Get the data structures right so downstream code becomes obvious.
中文简介正在准备中。当前显示原文说明。
Designs worksheets, workbooks and practice material that actually teach — sequencing difficulty, managing cognitive load, writing instructions a child can follow unaided, and building answer keys that hold up. Use this to design or fix a worksheet or workbook, decide how much practice a skill needs, structure a page so it can be completed without an adult reading it aloud, or diagnose material that looks right and does not work.
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