“conversion”的搜索结果

Map N6-methyladenosine (m6A) RNA modifications at single-nucleotide resolution using miCLIP (Linder 2015), miCLIP2 + m6Aboost machine learning (Kortel 2021), GLORI (Liu 2023, antibody-free chemical conversion), DART-seq (Meyer 2019, APOBEC1-YTH fusion), m6Anet (nanopore direct RNA), or MeRIP-seq with calibration. Use when distinguishing antibody-based from antibody-free m6A detection methods, applying the DRACH motif constraint, reconciling cross-method disagreements (DART 44% in DRACH vs GLORI), or detecting m6Am at the cap.

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Master AngularJS to Angular migration, including hybrid apps, component conversion, dependency injection changes, and routing migration.

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Production pipeline for ASCII art video — any format. Converts video/audio/images/generative input into colored ASCII character video output (MP4, GIF, image sequence). Covers: video-to-ASCII conversion, audio-reactive music visualizers, generative ASCII art animations, hybrid video+audio reactive, text/lyrics overlays, real-time terminal rendering. Use when users request: ASCII video, text art video, terminal-style video, character art animation, retro text visualization, audio visualizer in ASCII, converting video to ASCII art, matrix-style effects, or any animated ASCII output.

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Conversion-focused design that removes friction and guides users toward action through clarity, trust, and speed.

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When the user wants to optimize any form that is NOT signup/registration — including lead capture forms, contact forms, demo request forms, application forms, survey forms, or checkout forms. Also use when the user mentions "form optimization," "lead form conversions," "form friction," "form fields," "form completion rate," "contact form," "nobody fills out our form," "form abandonment," "too many fields," "demo request form," or "lead form isn't converting." Use this for any non-signup form that captures information. For signup/registration forms, see signup-flow-cro. For popups containing forms, see popup-cro.

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When the user wants to plan, design, or implement an A/B test or experiment. Also use when the user mentions "A/B test," "split test," "experiment," "test this change," "variant copy," "multivariate test," "hypothesis," "should I test this," "which version is better," "test two versions," "statistical significance," or "how long should I run this test." Use this whenever someone is comparing two approaches and wants to measure which performs better. For tracking implementation, see analytics-tracking. For page-level conversion optimization, see page-cro.

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Static-to-EDS conversion that preserves the original design while making content authorable in Document Authoring. Two modes — page-level (overlay template with slot markers) and block-level (each section becomes an independent EDS block). Use when converting an AI-generated static HTML page (Stardust, Mobirise, Relume, Lovable, v0, Figma-derived hand-coded, etc.) into an Edge Delivery Services page. Triggers on "convert this page to EDS", "static-to-EDS overlay", "convert to EDS blocks", "next experimentation", "next run", "start run", or when a user provides a source URL and asks to make it editable in DA while keeping the original design intact. Do NOT use for canonical EDS block-rewrite migrations — that's the page-import skill.

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Compares the performance of two or more audience segments across key metrics side by side. Use this skill when someone wants to compare audiences or visitor groups — for example, "how do mobile visitors compare to desktop on conversion," "compare new vs. returning visitors," "show me the difference between these two segments," "compare these audiences on our KPIs," or "which segment performs better." Also trigger for "segment comparison" or "audience comparison."

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Compares the performance of two or more audience segments across key metrics side by side. Use this skill when someone wants to compare audiences, cohorts, or groups — for example, "how do mobile users compare to desktop users on conversion," "compare new vs. returning visitors," "show me the difference between these two segments," "compare these audiences on our KPIs," or "which segment performs better." Also trigger for "segment comparison," "audience comparison," or "cohort comparison."

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Elite frontend image-direction skill for generating premium, conversion-aware website design references. CRITICAL OUTPUT RULE — generate ONE separate horizontal image FOR EVERY section. A landing page with 8 sections produces 8 images. Never compress multiple sections into one image. Enforces composition variety (not always left-text / right-image), background-image freedom, varied CTAs, varied hero scales (giant / mid / mini minimalist), narrative concept spine, second-read moments, and a single consistent palette across all images. Optimized for landing pages, marketing sites, and product comps that developers or coding models can accurately recreate.

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HTML -> UCUF (Cocos Creator UI) conversion skill. Use for turning a complete HTML source package with ui-design-tokens.json and colors_and_type.css into Cocos usable layout/skin/screen JSON, then validating with rule guard, visual fidelity risk, runtime interaction smoke, Cocos Editor final gate, and regression evidence.

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HTML -> UCUF (Cocos Creator UI) conversion skill. Use for turning a complete HTML source package with ui-design-tokens.json and colors_and_type.css into Cocos usable layout/skin/screen JSON, then validating with Plan5 final fidelity gates: stale-rule audit, rule guard, visual fidelity risk, runtime interaction smoke, Cocos Editor final gate, and 95% regression matrix.

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Master AngularJS to Angular migration, including hybrid apps, component conversion, dependency injection changes, and routing migration.

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Parses, queries, converts, and extracts from GTF and GFF3 gene-model annotation files - walking the gene/transcript/exon/CDS hierarchy with gffutils (queryable SQLite DB), converting formats and extracting transcript/CDS/protein FASTA with gffread, slurping to dataframes with gtfparse/pyranges, and sanitizing malformed files with AGAT. Covers the 1-based-inclusive vs 0-based BED coordinate conversion (start-1 only), deriving implicit features (introns/UTRs/TSS), phase-not-frame, the stop-codon-in-or-out-of-CDS convention, and the chr1-vs-1 seqid and gene-ID-version mismatches that silently produce all-zero count matrices and dropped joins. Use when extracting features or sequences from an annotation, converting GTF<->GFF3 or GTF->BED, traversing the gene tree, or diagnosing a coordinate/provenance mismatch upstream of counting or DE.

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Loads, converts, and manipulates Hi-C contact matrices in cooler format (.cool/.mcool/.scool) and Juicer .hic, using cooler (Python + CLI), hic2cool, and hictk. Covers the single-resolution mcool URI (file.mcool::/resolutions/<bp>), the load-bearing divisive-vs-multiplicative weight-naming rule (KR/VC/VC_SQRT auto-divisive vs cooler's multiplicative weight), what survives .hic<->.cool conversion (FRAG matrices and norm vectors do not), raw-vs-balanced coarsening, the .pairs upper-triangle/chromsize-order contract, and chrom-naming/bin-table provenance. Use when loading a cooler, converting .hic to .mcool, selecting a resolution, building a cooler from pairs or a matrix, coarsening/zoomifying, importing Juicer norm vectors, or debugging all-NaN balanced matrices and chr1-vs-1 empty fetches.

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Designs pegRNAs and nicking guides for prime editing (PE) -- choosing the nick/strand, tuning the primer-binding site (PBS) and reverse-transcription template (RTT) as a per-locus panel, selecting the PE system (PE2/PE3/PE3b/PE4/PE5/PEmax/PE7), adding MMR-evading and PAM-disrupting silent edits, appending epegRNA 3' motifs (tevopreQ1/mpknot), and ranking with PRIDICT/DeepPrime. Covers twinPE/PASTE for large insertions and the prime-vs-base-editing decision. Use when designing a scarless point mutation, small insertion/deletion, or any of the 12 base conversions without a double-strand break, when efficiency is low and MMR inhibition or pegRNA stabilization is needed, or when routing a large insertion to an integrase method. Generic guide scoring and base editing are separate skills.

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Stores and operates on sparse expression matrices for single-cell and large bulk RNA-seq, covering dgCMatrix/dgRMatrix/dgTMatrix when-each-is-fast, the dgCMatrix (CSC, R) <-> CSR (Python) implicit transpose, AnnData (cells-rows) <-> SingleCellExperiment (cells-cols) orientation flip, HDF5/h5ad vs Zarr cloud-native shift, HDF5SummarizedExperiment + DelayedArray for out-of-memory bulk, scanpy backed mode for large h5ad, the ~10-15% density crossover where dense beats sparse, 10X format proliferation (MTX vs CellRanger H5 vs h5ad), the dense-conversion memory blow-up, and Dask + Zarr for consortium-scale matrices. Use when choosing sparse format, working with single-cell-sized matrices, importing/exporting 10X, debugging R/Python interop transposes, processing matrices too large for RAM, or building cloud-native pipelines.

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Download genome assemblies, gene records, and ortholog data from NCBI using the modern Datasets v2 CLI (replaces assembly_summary.txt scraping and many EFetch workflows). Use when bulk-pulling genome assemblies, gene metadata across species, ortholog sets, or BLAST databases; when E-utilities are too slow for genome-scale work; or when automatic checksum verification, parallel download, and clean accession-driven retrieval are required. Encodes the JSON-lines output format, dataformat conversion, --dehydrated for cloud workflows, and when Datasets is/isn't the right tool.

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Quantifies CRISPR editing outcomes with CRISPResso2 (Clement 2019 Nat Biotechnol) across Cas9-nuclease (indels, HDR), CBE and ABE base editors (target conversion + bystander), and prime editor (pegRNA-templated) modes. Covers single-amplicon (CRISPResso), multi-sample batch (CRISPRessoBatch), pooled-amplicon (CRISPRessoPooled), WGS off-target (CRISPRessoWGS), and sample-comparison (CRISPRessoCompare) workflows; quantification-window math that controls what is called edited; substitution-vs-indel diagnostic to distinguish BE from Cas9 contamination; MMEJ deletion pattern interpretation; allele-frequency tables; and failure modes from amplicon misalignment or contamination. Use when quantifying editing from amplicon sequencing, choosing CRISPResso mode by design, distinguishing intended edits from bystanders and indel byproducts, debugging low-alignment runs, or generating publication-grade editing reports.

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Map N6-methyladenosine (m6A) RNA modifications at single-nucleotide resolution using miCLIP (Linder 2015), miCLIP2 + m6Aboost machine learning (Kortel 2021), GLORI (Liu 2023, antibody-free chemical conversion), DART-seq (Meyer 2019, APOBEC1-YTH fusion), m6Anet (nanopore direct RNA), or MeRIP-seq with calibration. Use when distinguishing antibody-based from antibody-free m6A detection methods, applying the DRACH motif constraint, reconciling cross-method disagreements (DART 44% in DRACH vs GLORI), or detecting m6Am at the cap.

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Generate, fill, and assemble PDF documents at scale. Handles legal forms, contracts, invoices, certificates. Supports form filling (pdf-lib), template rendering (Puppeteer, LaTeX), digital signatures (DocuSign), and document assembly. Use for legal tech, HR automation, invoice generation. Activate on "PDF generation", "form filling", "document automation", "digital signatures". NOT for simple PDF viewing, basic file conversion, or OCR text extraction.

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Image optimization analysis for SEO and performance. Checks alt text, file sizes, formats, responsive images, lazy loading, CLS prevention, image SERP rankings (via DataForSEO), and image file optimization (WebP/AVIF conversion, IPTC/XMP metadata injection). Use when user says "image optimization", "alt text", "image SEO", "image size", "image audit", "optimize images", "image metadata", "image SERP", "convert to webp", or "image file optimize".

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Generate SEO-optimized competitor comparison and alternatives pages. Covers "X vs Y" layouts, "alternatives to X" pages, feature matrices, schema markup, and conversion optimization. Use when user says "comparison page", "vs page", "alternatives page", "competitor comparison", "X vs Y", "versus", "compare competitors", or "alternative to".

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No-loss protocol for changing an existing mandate's output contract. Use when changing what an agent or mandate emits or how it is typed: binding a kind, declaring output_kind, kind-backfill batches, provision reconciliation, or any make-this-contract-honest sweep. NOT for creating a new agent (use create-agent).

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