237 个 · 按相关度排序
当需要为 AI 定义工具接口、设计调用规范、实现工具发现与编排机制时调用此 skill。典型场景包括：设计 AI agent 的工具集、定义 JSON Schema/XML/TypeScript 格式的工具描述、实现工具权限控制与并行调度、设计子代理委托架构。 不适用于：定义 AI 身份（persona-design）、安全规则（safety-guardrails）、记忆存储（memory-system）。 关键 trigger 信号：AI 需要调用外部 API 或服务、工具数量超过 10 个需要发现机制、需要区分工具权限层级、需要子代理并行执行、工具调用涉及敏感操作需要审批。
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End-to-end DNA sequencing workflow from FASTQ files to variant calls. Covers QC, alignment with BWA, BAM processing, and variant calling with bcftools or GATK HaplotypeCaller. Use when calling variants from raw sequencing reads.
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You are an expert in the Vercel AI SDK, the TypeScript toolkit for building AI-powered applications. You help developers integrate LLMs (OpenAI, Anthropic, Google, Mistral, Ollama) with React Server Components, streaming UI, tool calling, structured output with Zod schemas, RAG pipelines, multi-step agents, and edge-compatible AI features — the standard way to add AI to Next.js, Nuxt, SvelteKit, and any Node.js app.
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Expert in designing and building autonomous AI agents. Masters tool use, memory systems, planning strategies, and multi-agent orchestration. Use when: build agent, AI agent, autonomous agent, tool use, function calling.
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LLM 集成全流程。覆盖 OpenAI / Anthropic / Google / 开源模型接入、Prompt 工程、 Function Calling、Agent 编排、Token 优化、安全防护、生产化部署。 触发关键词：LLM、GPT、Claude、Gemini、Prompt、Function Calling、Tool Use、 Agent、Chain、Structured Output、Streaming、Token、Rate Limit。
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Discover, parse, and call external HTTP APIs at runtime using OpenAPI specs, tool templates, and iterative chaining. Adapted from UTCP (Universal Tool Calling Protocol) patterns for Node.js and Claude Code agents.
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Build LLM applications with chain-style orchestration frameworks — prompts, chains, tool-calling agents, memory, and retrieval pipelines. Use when structuring multi-step LLM programs, adding tools to a model, or composing RAG systems in code.
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Provider-agnostic patterns for LLM function calling, tool loops, and agentic workflows
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Execute ENCODE Whole Genome Bisulfite Sequencing (WGBS) pipeline from FASTQ to methylation calls. Child of pipeline-guide. Provides Nextflow execution with Docker and cloud deployment. Use when processing WGBS/bisulfite-seq data, calling methylation levels, generating bedMethyl files. Trigger on: WGBS pipeline, bisulfite sequencing, methylation calling, DNA methylation pipeline, bismark, bwa-meth, bedMethyl.
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Execute ENCODE Hi-C pipeline from FASTQ to contact matrices and loop calls. Child of pipeline-guide. Provides Nextflow execution with Docker and cloud deployment. Use when processing Hi-C data, generating contact matrices, or calling loops. Trigger on: Hi-C pipeline, chromatin conformation, contact matrix, loop calling, TAD detection, Juicer, HiCCUPS, 3D genome.
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Execute ENCODE ChIP-seq processing pipeline from FASTQ to peaks and signal tracks. Child of pipeline-guide. Provides stage-by-stage Nextflow execution with Docker containers and cloud deployment. Use when users need to process ChIP-seq data following ENCODE standards, run peak calling with MACS2, perform IDR analysis, or generate signal tracks. Trigger on: ChIP-seq pipeline, run ChIP-seq, process ChIP-seq, MACS2 peak calling, IDR analysis, ChIP-seq FASTQ processing.
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Persistent vector memory for Claude Code via LanceDB. Use when: remembering across sessions, storing lessons, recalling past decisions, maintaining context continuity. Tools: memory_store, memory_recall, memory_update, memory_forget, memory_merge.
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Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for visualisation.
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Expert in designing and building autonomous AI agents. Masters tool use, memory systems, planning strategies, and multi-agent orchestration. Use when: build agent, AI agent, autonomous agent, tool use, function calling.
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End-to-end Hi-C analysis workflow from FASTQ to compartments, TADs, and loops, with the decision of WHICH features the sequencing depth can support. Covers pairtools read-pair processing and library QC, cooler matrices, ICE balancing and distance-decay expected, A/B compartments, TAD boundaries, loop calling, and the routing of HiChIP/PLAC-seq/Capture Hi-C to protein-directed loop callers. Use when processing Hi-C data end to end, deciding a resolution for a given depth, or choosing between bulk-Hi-C and protein-directed loop calling.
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OpenAI API (developers.openai.com) の Responses API リファレンス。 text generation, embeddings, gpt-6-astra / gpt-6.1-sol / gpt-6-luna, structured outputs, streaming, function calling, programmatic / async tool calling, conversation state, prompt caching, prompt cache diagnostics, reasoning (configuration_update), background mode, compaction, multi-agent, WebSocket mode (mid-turn steering, stream_id), webhooks, error codes, rate limits, Realtime server controls, 公式 SDK (Python / JS / .NET / Java / Go / Ruby), openai CLI, Chat Completions / Assistants 移行, deprecations。
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Generate Skills from HTTP MCP servers with async job patterns (submit/status/result). Use when converting MCP specifications (.mcp.json) into reusable Skills using mcp_tool_catalog.yaml. Supports --lazy mode for context-saving generation. Also use for calling async MCP tools via JSON-RPC 2.0 with session-based polling.
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Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq c_curve / lc_extrap, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT — see Tips), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for downstream cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for downstream visualisation.
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Generate pileup data for variant calling using samtools mpileup and pysam. Use when preparing data for variant calling, analyzing per-position read data, or calculating allele frequencies.
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Use when building MCP (Model Context Protocol) servers in Java/Spring Boot. Covers tool registration, resource exposure, prompt templates, and production deployment using the official MCP Java SDK. Use when user mentions MCP, AI agent integration, or tool calling.
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Automate Callingly tasks via Rube MCP (Composio). Always search tools first for current schemas.
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Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq c_curve / lc_extrap, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT — see Tips), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for downstream cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for downstream visualisation.
中文简介正在准备中。当前显示原文说明。
Generate pileup data for variant calling using samtools mpileup and pysam. Use when preparing data for variant calling, analyzing per-position read data, or calculating allele frequencies.
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Build with OpenAI's stateless APIs - Chat Completions (GPT-5, GPT-4o), Embeddings, Images (DALL-E 3), Audio (Whisper + TTS), and Moderation. Includes Node.js SDK and fetch-based approaches for Cloudflare Workers. Use when: implementing chat completions with GPT-5/GPT-4o, streaming responses with SSE, using function calling/tools, creating structured outputs with JSON schemas, generating embeddings for RAG (text-embedding-3-small/large), generating images with DALL-E 3, editing images with GPT-Image-1, transcribing audio with Whisper, synthesizing speech with TTS (11 voices), moderating content (11 safety categories), or troubleshooting rate limits (429), invalid API keys (401), function calling failures, streaming parse errors, embeddings dimension mismatches, or token limit exceeded.
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