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Plan and document studies that assess whether an analytical procedure suits its intended use. Select the governing framework, evaluate calibration, accuracy, precision, and detection limits, and compare methods using acceptance criteria defined before data collection.

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• Planning analytical validation studies and acceptance criteria
• Evaluating calibration, accuracy, and precision
• Estimating and confirming detection and quantitation limits

Develop a high-performance liquid chromatography method: define separation goals, select column chemistry and mobile phase, optimize gradient and flow conditions, choose the appropriate detector, and evaluate initial method performance for target analytes in solution or complex matrices.

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Think and work like an expert Food Chemist. Use when a task calls for Food Chemist judgment. Reasons from food matrix effects, aw and lipid oxidation, AOAC-validated HPLC/GC-MS/LC-MS/MS, FoodData Central/FNDDS, and trained sensory panels while treating matrix suppression, accelerated-shelf-life misuse, and untrained-taster data as first-class failure modes.

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Think and work like an expert Analytical Chemist. Use when a task calls for Analytical Chemist judgment. Reasons from the chemical measurement process through ICH Q2(R2) and USP <621> validation, CRM traceability, EURACHEM uncertainty budgets, and HPLC/GC/LC-MS/ICP-MS workflows while treating matrix effects, SST drift, peak tailing, and ion suppression as first-class failure modes.

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Interpret a chromatogram from GC or HPLC analysis: verify system suitability parameters, identify peaks by retention time and spectral matching, perform accurate peak integration, calculate chromatographic figures of merit, and assess overall peak quality for reliable quantitation.

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Use when a task needs the judgment of a Chemist — developing or validating an analytical method (HPLC, GC, spectroscopy), designing a synthesis route and reconciling a reaction yield, diagnosing an out-of-specification (OOS) lab result, interpreting a spectrum to confirm structure or purity, or reviewing a stability/degradation study.

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Plan, execute, and document validation, verification, and transfer of analytical procedures under the governing framework - ICH Q2(R2) and Q14, USP <1220>/<1225>/<1226>, ICH M10 bioanalytical, CLSI EP, or ISO/IEC 17025. Use for HPLC, LC-MS/MS, GC, CE, ICP-MS, dissolution, qNMR, qPCR, NIR, and ligand binding or cell-based assays whenever the question is whether a procedure is fit for its intended purpose. Triggers include "method validation", "analytical method validation", "AMV", "validation protocol", "acceptance criteria", "linearity", "reportable range", "accuracy and precision", "repeatability", "intermediate precision", "recovery", "LOD", "LOQ", "detection limit", "quantitation limit", "specificity", "robustness", "method transfer", "method comparison", "Deming", "Passing-Bablok", "Bland-Altman", "equivalence testing", "OOS investigation", "ICH Q2", "Q2(R2)", "Q14", "USP 1225", "ICH M10", "incurred sample reanalysis", "ISR", "CLSI EP", and any request to show that an assay works.

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Expert-level analytical chemistry knowledge. Use when working with chromatography, spectroscopy, electroanalytical methods, sample preparation, quantitative analysis, calibration, statistics, or instrumental analysis. Also use when the user mentions 'HPLC', 'GC', 'mass spectrometry', 'atomic absorption', 'ICP', 'titration', 'calibration curve', 'detection limit', 'NMR', 'chromatography', 'electrochemistry', 'sample preparation', or 'method validation'.

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Plan, execute, and document validation, verification, and transfer of analytical procedures under the governing framework - ICH Q2(R2) and Q14, USP <1220>/<1225>/<1226>, ICH M10 bioanalytical, CLSI EP, or ISO/IEC 17025. Use for HPLC, LC-MS/MS, GC, CE, ICP-MS, dissolution, qNMR, qPCR, NIR, and ligand binding or cell-based assays whenever the question is whether a procedure is fit for its intended purpose. Triggers include "method validation", "analytical method validation", "AMV", "validation protocol", "acceptance criteria", "linearity", "reportable range", "accuracy and precision", "repeatability", "intermediate precision", "recovery", "LOD", "LOQ", "detection limit", "quantitation limit", "specificity", "robustness", "method transfer", "method comparison", "Deming", "Passing-Bablok", "Bland-Altman", "equivalence testing", "OOS investigation", "ICH Q2", "Q2(R2)", "Q14", "USP 1225", "ICH M10", "incurred sample reanalysis", "ISR", "CLSI EP", and any request to show that an assay works.

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