“saturation”的搜索结果

Calculate seawater carbonate chemistry from two independent measurements using PyCO2SYS. Produce pH, carbonate species, and mineral saturation results, convert laboratory conditions to ocean conditions, and propagate measurement uncertainty while recording calculation assumptions.

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• Calculate carbonate chemistry from paired seawater measurements
• Assess aragonite and calcite saturation
• Convert laboratory results to ocean temperature and pressure

Plan user research by matching methods to study goals and explaining participant numbers and confidence. Synthesize tagged observations into recurring findings, distinguish isolated anecdotes, and organize evidence so research results can be found and reused.

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• Choose a research method for a study goal
• Explain participant numbers and confidence
• Find recurring patterns across participants

Maps whether a biomedical research topic, subtopic, or study angle is truly saturated, superficially crowded, strategically occupied, or still open for differentiated entry. Use this skill when a user wants to know whether a hot medical research direction is already overworked, whether meaningful whitespace remains, whether major groups have already occupied the obvious claims, and whether the timing window is still open. Always distinguish popularity from true saturation, and distinguish cosmetic novelty from meaningful differentiating entry.

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Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for visualisation.

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Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for visualisation.

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Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq c_curve / lc_extrap, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT — see Tips), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for downstream cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for downstream visualisation.

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Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq c_curve / lc_extrap, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT — see Tips), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for downstream cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for downstream visualisation.

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Investigate a topic with cooperating agents that divide the research, challenge findings, and track evidence. Produce a cited synthesis with an auditable research record, verification artifacts where needed, and explicit treatment of unresolved or refuted claims.

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• Investigating a topic from several perspectives
• Checking conflicting claims against evidence
• Researching code alongside external documentation

Mix audio already placed in HyperFrames compositions. Adjust fades, volume changes, and effects; reduce music frequencies that compete with speech; and apply shared processing to multiple tracks through a group with common volume controls.

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• Make narration easier to hear over background music
• Add fades and crossfades to placed audio
• Balance uneven voice levels and adjust tone

Research a topic from multiple angles, preserve sources according to your privacy preferences, and summarize each one. Combine the evidence into a self-contained reference page, including limitations and counterarguments, with options to deepen the research later.

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• Building a reference guide on a new topic
• Keeping sources alongside individual summaries
• Comparing evidence, counterarguments, and limitations

Investigate slow TPU training on GKE by comparing diagnostic events with one-minute monitoring metrics. Separate memory or CPU bottlenecks from hardware and network issues, and identify the appropriate next steps.

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• Investigating slower TPU training without pod crashes
• Checking PERFORMANCE_DEGRADATION events
• Separating resource bottlenecks from infrastructure issues

Create and validate Terraform alerts for GKE using PromQL, a monitoring query language. Cover response times, errors, traffic, memory usage, and cluster health, with checks for alert timing, duplicate signals, and metric ingestion costs.

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• Create alerts for GKE service latency and errors
• Detect frequent container restarts and unhealthy nodes
• Review existing Terraform alert configurations

Retrieve BigQuery execution records and resource metrics using the right tool for each task. The skill guides monitoring tool selection and SQL queries for performance investigations, capacity analysis, and reviews of billable compute and storage usage.

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• Retrieve execution details for a slow job
• Check compute capacity contention and queue delays
• Review reserved capacity and autoscaling limits

Generate Terraform alerts for Cloud Run services, jobs, and worker pools using PromQL, a monitoring query language. Monitor errors, latency, resource usage, job failures, and queue delays, with configurable thresholds and explanations of when each alert triggers.

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• Monitor Cloud Run service errors and latency
• Detect failed batch job executions
• Monitor worker pool backlogs and message age

Creates bold interface components with thick black borders, vivid colors, sharp corners, and solid offset shadows. Uses shared CSS settings to coordinate cards, buttons, navigation, inputs, and badges, including interaction states and accessibility considerations.

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• Creating cards with bold borders and solid shadows
• Adding hover and pressed states to buttons
• Coordinating navigation, inputs, and badges

Agent E2 - Qualitative Coding Specialist - Systematic coding and theme development. Covers codebook development, coding strategies, saturation assessment, and CAQDAS guidance.

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Run stepped HTTP load tests with ab/wrk, ramping concurrency levels to collect p50/p90/p99 latency, detect performance inflection points, and recommend optimal concurrency. Triggered by requests like 'load test this URL', 'benchmark my API', 'find the max concurrency', or mentions of p99 latency, throughput saturation, or capacity planning.

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Runs maximum-saturation research with a cooperating team, claim-graph gating, and a cited, QA'd deliverable. Use when the user explicitly asks for research or a deep investigation, including any 'ulw' research wording.

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[omh] Deep dive before a decision: deep research engine - grounding for specs and decisions: study open-source reference implementations with pinned refs, gather live web evidence with citation discipline, verify contested claims, and distill a decision-grounding dossier that planning consumes; for a decision brief use research-brief, for upstream guidance use web-research. Use when the user says: research plan, literature review, research literature, review recent papers, deep research, deep-research, exhaustive research, saturation research.

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Score, annotate, and analyze the functional impact of genetic variants using AlphaGenome Variant Impact (AVI) scores. Query variants in chr:pos:ref>alt format, annotate VCF/tabular callsets, perform saturation mutagenesis window scans (1-based closed chr:start-end), and extract GENCODE v46 GTF gene/exon/junction coordinates all via the AlphaGenome Atlas API.

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Adapt an existing palette to dark mode — surface elevation, contrast rebalancing, and desaturation rules. Use when you already have a light palette to translate. For building the base palette first, use `color-system`.

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Use this skill when the user asks to summarize, or asks why Simulink Coverage objectives are missing, unsatisfied, or uncovered on a coverage result they already have — why specific decision, condition, MCDC, relational-boundary, or saturation/overflow outcomes weren't exercised, whether uncovered logic is dead code, or how to close the gap (a test, a coverage filter, or a design change). It reads an existing cvdata, .cvt file, sim-with-coverage output, or Simulink Test result; it never runs cvsim to collect coverage.

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Designs and analyzes pooled prime-editor (PE) screens for installing precise genetic variants without bystander confounding. Covers pegRNA design with PRIDICT and PRIDICT2 for predicting per-pegRNA editing efficiency, pegRNA architecture (spacer + scaffold + PBS + RTT), PE2/PE3/PE3b/PEmax variants, MOSAIC in situ saturation mutagenesis, the PRIME pooled-screen methodology (Ren 2023; ~3,699 ClinVar variant screens), chromatin context as a major locus-level determinant of PE efficiency, scaffold-incorporation and indel byproduct quantification with CRISPResso2, and the cross-modal validation strategy of PE + base-editor screens for variant function. Use when designing a pegRNA library for variant installation, choosing between BE and PE for a specific edit, predicting pegRNA efficiency before library synthesis, analyzing PE screen output, distinguishing intended-edit from scaffold-incorporation, or scaling PE screens to thousands of variants.

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Analyzes base-editing screens for variant function. Covers library design (Hanna 2021 ClinVar-scale CBE screen benchmarked on BRCA1/2, Cuella-Martin 2021 DDR saturation), CBE vs ABE chemistry choice (BE3/BE4 vs ABE7.10/ABE8.20/ABE8e), editing-window math (positions 4-8 from PAM-distal end; 4-7 for ABE7.10), bystander-edit quantification and the variant-call ambiguity it creates, sgRNA-efficiency filtering before hit calling, indel byproduct interpretation, the substitution-vs-indel diagnostic, variant annotation against ClinVar / COSMIC, and the Broad be-validation-pipeline. Use when designing a BE variant screen, choosing CBE vs ABE for a specific edit, interpreting bystander-confounded hits, distinguishing functional signal from indel artifact, integrating CRISPResso2 output with screen scoring, or deciding BE vs PE for SNV installation.

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