その他のスキル

Quantifies alternative splicing as PSI (percent spliced in) from RNA-seq using rMATS-turbo (BAM-based event), SUPPA2 (TPM-based event), MAJIQ V3 (LSV-based Bayesian), leafcutter (annotation-free intron clusters), VAST-TOOLS (cross-species with microexon support), Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage), or IRFinder-S (intron retention coverage-aware). Distinguishes the five canonical event classes (SE, A5SS, A3SS, MXE, RI), special classes (microexons, exitrons, AFE/ALE), intron retention subtypes (canonical RI vs detained introns), and applies effective-length normalization. Use when measuring splice-site usage or isoform inclusion ratios from short-read RNA-seq.

日本語の概要は準備中です。原文の説明を表示しています。

Detect allele-specific chromatin accessibility from ATAC-seq using WASP, GATK ASEReadCounter, or RASQUAL. Use when mapping cis-regulatory genetic variants from heterozygous SNPs, separating cis from trans regulation, building chromatin QTL (caQTL) maps, validating GWAS variant function with allelic imbalance, or detecting reference allele mapping bias before downstream analysis.

日本語の概要は準備中です。原文の説明を表示しています。

Generate consensus sequences and manage reference files using samtools. Use when creating consensus from alignments, indexing references, or creating sequence dictionaries.

日本語の概要は準備中です。原文の説明を表示しています。

Sort alignment files by coordinate or read name using samtools and pysam. Use when preparing BAM files for indexing, variant calling, or paired-end analysis.

日本語の概要は準備中です。原文の説明を表示しています。

Filter alignments by flags, mapping quality, and regions using samtools view and pysam. Use when extracting specific reads, removing low-quality alignments, or subsetting to target regions.

日本語の概要は準備中です。原文の説明を表示しています。

Trim PCR primers from aligned reads in amplicon-panel BAMs using samtools ampliconclip. Use when processing SARS-CoV-2 ARTIC, hereditary cancer panels, ctDNA hot-spot panels, or any amplicon assay where primer-derived bases would falsely confirm reference at primer footprints.

日本語の概要は準備中です。原文の説明を表示しています。

Infer cis-regulatory connections (peak-to-peak co-accessibility) from scATAC-seq using Cicero, ArchR getCoAccessibility, or SCENIC+. Use when linking enhancer accessibility to promoter accessibility, identifying enhancer-gene pairs from chromatin alone (without paired RNA), running gene-regulatory inference combining ATAC + RNA, or comparing predicted regulatory contacts against Hi-C/Micro-C ground truth.

日本語の概要は準備中です。原文の説明を表示しています。

LSF 作业内存不足时的调整技能。当作业因内存不够被挂起(SSUSP)或终止(EXIT/MEMLIMIT)时，通过调整 bsub -n 的节点（核数/slot）数量来扩大可用内存——可用运行内存 ≈ 5GB × 申请节点数。当用户说内存不足、作业被杀、OOM、需要更大内存，或排查 EXIT/SSUSP 作业时，配合 lsf-ncpgr skill 一起使用。

日本語の概要は準備中です。原文の説明を表示しています。

Detect transcription factor binding footprints in ATAC-seq using TOBIAS, HINT-ATAC, Wellington, or scprinter. Use when identifying bound TF sites within accessible regions, correcting Tn5 insertion bias before footprinting, choosing between cleavage-based and aggregate-based footprinters, or comparing differential TF activity between conditions.

日本語の概要は準備中です。原文の説明を表示しています。

生成图文并茂的分析报告/实验报告/质检报告（固定模版）。当用户要求"生成报告/总结报告/实验报告/质检报告/QC报告"，或分析流程收尾需要交付报告时使用。报告必须基于真实分析输出，包含固定章节结构、关键指标表格和真实结果图。

日本語の概要は準備中です。原文の説明を表示しています。

NCPGR高性能计算集群LSF作业调度系统使用指南。用于提交、管理和监控LSF批处理作业，包括串行作业、多线程作业、MPI并行作业、GPU作业、数组作业等。当用户需要编写LSF作业脚本、提交计算任务、查询作业状态、调整作业参数或排查作业问题时使用此skill。

日本語の概要は準備中です。原文の説明を表示しています。

Select, document, download, and checksum-verify the most appropriate NCBI reference genome on an HPC cluster. Use whenever an analysis needs a reference genome, genome FASTA, genome annotation, assembly accession, or genome index and no exact approved assembly is already available; when a user asks to fetch a species genome from NCBI; or when a pipeline must resolve and cache a reproducible reference assembly on a remote cluster.

日本語の概要は準備中です。原文の説明を表示しています。

Use when a carrier or driver asks about winter trucking, snow chain requirements, hot-weather precautions, summer tire failures, fuel additive use (anti-gel), idle management in extreme temperatures, weight restrictions in spring thaw, or how seasonal demand patterns affect freight rates and capacity.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when selecting a TPR-registered Training Provider for behind-the-wheel ELDT training. Covers the registry, evaluation criteria, and common pitfalls.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill to evaluate document retention software options. Covers compliance features, ease of use, integration, and cost.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill for wheel-end maintenance and lubrication. Covers oil-bath vs grease packs, wheel-end loss issues, and inspection intervals.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when buying a used tractor or trailer. Covers verifying compliance status, hidden issues, and what to inspect before signing.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill for vehicle brake system inspection requirements. Covers daily DVIR, weekly maintenance, brake adjustment check, and the 'percent of out-of-adjustment' rule.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks about team driving — two-driver OTR operations, how teams maximize miles, HOS rules with sleeper berth swap, pay structures, equipment requirements (sleeper berth specs), team retention, and common challenges. Reference 49 CFR 395.1(g).

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks about state-specific trucking taxes BEYOND IFTA — NY Highway Use Tax (HUT), KY Kentucky Intrastate Tax (KIT), Oregon Weight-Mile Tax, New Mexico Weight-Distance Tax, Connecticut Highway Use Fee, or other state-level road-use taxes for commercial vehicles. Covers thresholds, rates, registration, filing cadence, and how these differ from IFTA. Reference state DOT/DOR resources.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill to understand record retention requirements for currently-employed drivers. Covers DQF, HOS, vehicle records, and D&A specific periods.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks about CMV vehicle inspections under 49 CFR 396 — DVIRs (Driver Vehicle Inspection Reports), pre-trip inspections, post-trip inspections, periodic inspections, annual inspections, who can perform inspections, OOS criteria, and what makes a "no defect" DVIR valid. Cite 49 CFR 396.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill for training drivers on effective pre-trip inspection technique. Covers the comprehensive walk-around, time investment, and common areas drivers miss.

日本語の概要は準備中です。原文の説明を表示しています。

Use when a carrier asks about trailer types — dry van, reefer, flatbed, step-deck, conestoga, tanker, dump, lowboy, intermodal chassis, auto-hauler. Covers length / weight rules, common specs, reefer unit options (Carrier vs Thermo King), liftgate options, kingpin setting, and trailer-to-tractor matching.

日本語の概要は準備中です。原文の説明を表示しています。