493 skills · sorted by relevance
Core Kubernetes resource management for pods, namespaces, configmaps, secrets, and nodes. Use when listing, inspecting, or managing fundamental K8s objects.
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Writes E2E tests with Playwright including test scripts, page objects, test fixtures, reporters, CI integration, API mocking, and visual regression testing.
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Bevy 0.20 collection loop: pickup markers, a quota resource, one score per entity per pass, and NextState when the quota is met. Use when the run is about collecting objects. Keywords: Bevy, collection, pickup, quota, NextState, ECS.
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Image segmentation is the process of identifying the borders of individual objects (in this case cells) within an image. This allows for the features of cells such as marker expression and morphology to be extracted, stored and analysed. simpleSeg provides functionality for user friendly, watershed based segmentation on multiplexed cellular images in R based on the intensity of user specified protein marker channels. simpleSeg can also be used for the normalization of single cell data obtained f
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Spatially-aware quality control (QC) software for both spot-level and artifact-level QC in spot-based spatial transcripomics, such as 10x Visium. These methods calculate local (nearest-neighbors) mean and variance of standard QC metrics (library size, unique genes, and mitochondrial percentage) to identify outliers spot and large technical artifacts. Scales linearly with the number of spots and is designed to be used with 'SpatialExperiment' objects.
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Provides S4 classes for general nucleases, CRISPR nucleases, CRISPR nickases, and base editors.Several CRISPR-specific genome arithmetic functions are implemented to help extract genomic coordinates of spacer and protospacer sequences. Commonly-used CRISPR nuclease objects are provided that can be readily used in other packages. Both DNA- and RNA-targeting nucleases are supported.
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The epistack package main objective is the visualizations of stacks of genomic tracks (such as, but not restricted to, ChIP-seq, ATAC-seq, DNA methyation or genomic conservation data) centered at genomic regions of interest. epistack needs three different inputs: 1) a genomic score objects, such as ChIP-seq coverage or DNA methylation values, provided as a `GRanges` (easily obtained from `bigwig` or `bam` files). 2) a list of feature of interest, such as peaks or transcription start sites, provi
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PhIPData defines an S4 class for phage-immunoprecipitation sequencing (PhIP-seq) experiments. Buliding upon the RangedSummarizedExperiment class, PhIPData enables users to coordinate metadata with experimental data in analyses. Additionally, PhIPData provides specialized methods to subset and identify beads-only samples, subset objects using virus aliases, and use existing peptide libraries to populate object parameters.
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Mass spectrometry (MS) data backend supporting import and export of MS/MS library spectra from MassBank record files. Different backends are available that allow handling of data in plain MassBank text file format or allow also to interact directly with MassBank SQL databases. Objects from this package are supposed to be used with the Spectra Bioconductor package. This package thus adds MassBank support to the Spectra package.
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This R package supports interactive visualization of multi-channel images and segmentation masks generated by imaging mass cytometry and other highly multiplexed imaging techniques using shiny. The cytoviewer interface is divided into image-level (Composite and Channels) and cell-level visualization (Masks). It allows users to overlay individual images with segmentation masks, integrates well with SingleCellExperiment and SpatialExperiment objects for metadata visualization and supports image do
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This package implements sampling, iteration, and input of FASTQ files. The package includes functions for filtering and trimming reads, and for generating a quality assessment report. Data are represented as DNAStringSet-derived objects, an
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A set of tools for making TxDb objects from genomic annotations from various sources (e.g. UCSC, Ensembl, and GFF files). These tools allow the user to download the genomic locations of transcripts, exons, and CDS, for a given assembly, and
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Graph objects from pathway topology derived from KEGG, Panther, PathBank, PharmGKB, Reactome SMPDB and WikiPathways databases.
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Microbiome time series simulation with generalized Lotka-Volterra model, Self-Organized Instability (SOI), and other models. Hubbell's Neutral model is used to determine the abundance matrix. The resulting abundance matrix is applied to (Tree)SummarizedExperiment objects.
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Expert knowledge for Azure Data Api Builder development including troubleshooting, best practices, decision making, limits & quotas, security, configuration, integrations & coding patterns, and deployment. Use when exposing DB objects via REST/GraphQL, tuning paging/timeouts, securing auth/RLS, or deploying DAB to Azure, and other Azure Data Api Builder related development tasks. Not for Azure API Management (use azure-api-management), Azure App Service (use azure-app-service), Azure Functions (use azure-functions), Azure Logic Apps (use azure-logic-apps).
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Create animated, explorable editorial diagrams in 45 types (architecture, flowchart, sequence, state, ER, DB schema, UML class, swimlane, timeline, gantt, journey, tree, org chart, nested, venn, quadrant, fishbone, wardley, bar, line, scatter, sankey, treemap, heatmap, 3D isometric objects, floor plans and exploded views) as standalone HTML from a short JSON spec. Use when the user asks to draw, diagram, chart or visualize a system, codebase, flow, process, API call sequence, hierarchy, data or a physical object (a chair); to convert Mermaid, Graphviz/DOT, PlantUML, D2, draw.io, Excalidraw, SQL or CSV into a clean diagram; or to export a diagram as PNG, JPEG, SVG, GIF or MP4. Also for everyday plans: trips, moves, job hunts, approvals, budgets, where money goes.
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Transform ordinary snapshots—including daily moments, portraits, objects, pets, food, interiors, streets, architecture, landscapes, travel, and humanistic scenes—into the restrained Shijing Paper Zine visual style. Use when the user wants any real photo turned into a spacious paper-editorial composition with an irregular photographic fragment, simplified source-derived drawing, one structural print color, tactile torn-paper texture, minimal micro-copy, a consistent multi-image series, or a source-to-poster comparison.
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Use when generating mocks, stubbing methods, verifying interactions, capturing arguments, or choosing between mocks, fakes, and real objects (Mockito).
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Use when creating mocks, stubbing methods, verifying interactions, registering fallback values, or choosing between mocks, fakes, and real objects (Mocktail).
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Tactical DDD — aggregate design rules, entities, value objects, domain events, repositories, domain services, and anti-pattern detection
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Use when a user wants Codex to directly operate PowerPoint and reconstruct an entire reference image as editable PowerPoint objects while preserving its full layout, labels, connectors, and visual relationships. Do not use for paper-to-deck generation or VBA-only output.
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Generates Playwright page objects from an executable test plan, one class per screen with ladder-compliant locators and no assertions, wired into a shared fixture. Use when a web scenario needs a page object that does not exist yet, or when locators drift and the page object must be rebuilt.
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Use ManagedCode.Communication when a .NET application needs explicit result objects, structured errors, and predictable service or API boundaries instead of exception-driven control flow. USE FOR: integrating ManagedCode.Communication into services or APIs; replacing exception-driven result handling with explicit results; reviewing service boundaries that return. DO NOT USE FOR: unrelated stacks; generic tasks that do not need this specific guidance. INVOKES: inspect the repository context, edit targeted files, and run relevant build, test, lint, or validation commands when changes are made.
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Converts audio objects stored in Volcengine TOS into PCM-oriented WAV output through TOS data-process async jobs. Use this skill when the user needs to convert audio to PCM or WAV, resample audio to 16k, convert to mono, change sample format, normalize audio for ASR pipelines, or prepare TOS audio files for speech recognition — even if they don't explicitly mention 'PCM' or 'audio conversion'.
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