365 skills · sorted by relevance
Investigate and fix agent behavior in TradingAgentsCN by checking active database prompt templates, prompt assembly code, and output processing. Apply targeted changes and verify them with focused tests, template checks, and workflow logs.
Retrieve focused genome, assembly, and taxonomy metadata from NCBI Datasets v2, a biological data service. Get concise Markdown summaries by default, with raw JSON or text available when requested.
Convert an older Blazor Server app into a .NET 8+ Blazor Web App while preserving interactive server behavior. It guides changes to page structure, startup configuration, authentication state sharing, and validation of the converted app.
Assembles microbial-community sequencing into metagenome-assembled genomes (MAGs) with metaFlye (ONT), metaSPAdes/MEGAHIT (Illumina), and hifiasm-meta/metaMDBG (PacBio HiFi), then recovers genomes via multi-binner consolidation (MetaBAT2, MaxBin2, CONCOCT, SemiBin2, VAMB -> DAS_Tool) and QCs them against MIMAG with CheckM2, GUNC, and GTDB-Tk. Covers why a metagenome is not a genome (uneven coverage, micro-diversity, strain collapse to consensus), differential-coverage binning, co-assembly vs per-sample, the rRNA-operon collapse that fails short-read MAGs, and strain resolution with inStrain. Use when reconstructing genomes from a microbiome, soil, ocean, or gut community, recovering MAGs, or resolving strain-level variation.
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Assembles a genome de novo from Illumina short reads with SPAdes (isolate/careful/sc/meta/plasmid/rna modes), MEGAHIT (low-memory, huge datasets), Unicycler (bacterial finishing/hybrid), MaSuRCA (large hybrid), ABySS (Bloom-filter), and Platanus (heterozygous diploids), using multi-k de Bruijn graphs. Covers the repeat-resolution limit, why N50 plateaus at the genome not the depth, GenomeScope2 k-mer profiling first, the heterozygosity/haplotig trap, error-correction erasing rare alleles, GC dropout, and NG50/auN/BUSCO reporting. Use when assembling a bacterial isolate, fungal, small-eukaryotic, single-cell, or metagenome genome from Illumina reads, or when deciding whether short reads can even produce the assembly being asked for.
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Assembles haplotype-resolved diploid and telomere-to-telomere (T2T) genomes from PacBio HiFi reads with hifiasm (HiFi-only, Hi-C, or trio phasing) and verkko (HiFi + ultralong ONT for T2T), extracting contigs from GFA and routing phasing QC to k-mer/trio metrics. Covers why a primary assembly is a haplotype mosaic that exists in no cell, partial-vs-full phasing (the .bp. vs .dip. filename convention), the purge-default trap on inbred samples, the --hom-cov coverage-estimate alarm, and verkko-vs-hifiasm for T2T. Use when assembling a diploid eukaryote from HiFi, phasing haplotypes with parents (trio) or Hi-C, deciding whether to chase T2T, or diagnosing switch errors invisible to N50/BUSCO/QV.
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Detects and removes contamination in genome assemblies via two disjoint workflows - foreign-sequence screening of a single-organism (eukaryote/isolate) assembly with NCBI FCS-GX (GenBank-submission-mandatory), FCS-adaptor, and BlobToolKit blob plots; and MAG/bin quality assessment with CheckM2 plus GUNC (chimerism) plus GTDB-Tk taxonomy, judged against MIMAG. Covers why CheckM2 alone is blind to disjoint-marker chimeras, the FCS-GX RAM wall, organelle/NUMT triage, strain heterogeneity, and the HGT-vs-contamination (tardigrade) trap. Use when screening an assembly for foreign contamination before GenBank submission, assessing MAG completeness/contamination/chimerism, deciding which contigs to remove, or distinguishing real HGT from contaminant contigs.
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AssemblyAI integration. Manage Transcripts, Speakers, Jobs. Use when the user wants to interact with AssemblyAI data.
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Workflow for de novo assembly, scaffolding, polishing, contamination review, and assembly QC.
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AssemblyAI is a hosted speech-to-text API that transcribes audio and video files or live streams and adds speaker labels, sentiment, entity detection, PII redaction and LLM analysis of the transcript. Use when a user asks to transcribe a recording, label who said what, analyze call sentiment, redact personal data, stream live transcription, or summarize and question a transcript with LLM Gateway (the replacement for LeMUR).
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Expert in Ethereum Virtual Machine internals - gas optimization, assembly/Yul, opcode-level optimization, and low-level EVM patternsUse when "evm, gas optimization, yul, inline assembly, opcodes, solidity optimization, evm bytecode, storage layout, evm, ethereum, solidity, yul, assembly, gas-optimization, opcodes, smart-contracts" mentioned.
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语音转文字 (ASR) (语音转文字 (ASR / 语音识别 / Speech-to-Text) 应用工程 (从业者视角) — 选型、集成、优化语音转文字能力，尤其面向移动端、低成本、快速识别的场景。覆盖: (a) 引擎/API 地图 — 云端 API(OpenAI gpt-4o-transcribe / Whisper API、Deepgram、AssemblyAI、Google / Azure / AWS Transcribe、讯飞、字节火山、阿里、腾讯、百度) vs 开源模型(Whisper / faster-whisper / whisper.cpp / distil-whisper、NVIDIA NeMo Parakeet / Canary、阿里 FunASR / Paraformer / SenseVoice、Moonshine、Vosk、Kaldi / k2 / icefall) vs 端侧·移动 SDK(whisper.cpp + CoreML / Metal、iOS Speech framework、Android SpeechRecognizer、Picovoice、SenseVoice 端侧); (b) 准确率(WER / CER) × 延迟(RTF / 流式) × 成本 三角权衡与选型决策树; (c) 成本优化 playbook — 端侧免费 / 批量折扣 / VAD 裁静音 / 量化(int8 / ggml) / 蒸馏 / 自托管 break-even; (d) 移动端集成 — 端侧 vs 云、流式 vs 批量、断点检测(endpointing)、隐私 / 离线; (e) 后处理 — 标点 / 数字规整(ITN) / 说话人分离(diarization) / 时间戳。学派分歧: 云 API vs 端侧自托管、通用大模型(Whisper) vs 专用流式(RNN-T / Conformer)、闭源 API vs 开源、准确率派 vs 成本派、英文优先 vs 中文 ASR(FunASR / SenseVoice / 讯飞)。不含: 文字转语音(TTS / 语音合成，方向相反)、声纹识别 / 说话人验证为主业、语音 agent / 对话式 AI、ASR 模型训练科研深水区。) Master OS — automated mastery of 语音转文字 (ASR / 语音识别 / Speech-to-Text) 应用工程 (从业者视角) — 选型、集成、优化语音转文字能力，尤其面向移动端、低成本、快速识别的场景。覆盖: (a) 引擎/API 地图 — 云端 API(OpenAI gpt-4o-transcribe / Whisper API、Deepgram、AssemblyAI、Google / Azure / AWS Transcribe、讯飞、字节火山、阿里、腾讯、百度) vs 开源模型(Whisper / faster-whisper / whisper.cpp / distil-whisper、NVIDIA NeMo Parakeet / Canary、阿里 FunASR / Paraformer / SenseVoice、Moonshine、Vosk、Kaldi / k2 / icefall) vs 端侧·移动 SDK(whisper.cpp + CoreML / Metal、iOS Speech framework、Android SpeechRecognizer、Picovoice、SenseVoice 端侧); (b) 准确率(WER / CER) × 延迟(RTF / 流式) × 成本 三角权衡与选型决策树; (c) 成本优化 playbook — 端侧免费 / 批量折扣 / VAD 裁静音 / 量化(int8 / ggml) / 蒸馏 / 自托管 break-even; (d) 移动端集成 — 端侧 vs 云、流式 vs 批量、断点检测(endpointing)、隐私 / 离线; (e) 后处理 — 标点 / 数字规整(ITN) / 说话人分离(diarization) / 时间戳。学派分歧: 云 API vs 端侧自托管、通用大模型(Whisper) vs 专用流式(RNN-T / Conformer)、闭源 API vs 开源、准确率派 vs 成本派、英文优先 vs 中文 ASR(FunASR / SenseVoice / 讯飞)。不含: 文字转语音(TTS / 语音合成，方向相反)、声纹识别 / 说话人验证为主业、语音 agent / 对话式 AI、ASR 模型训练科研深水区。: top builders' mental models, tool stack, current workflows, jargon, and where to keep up. Trigger this skill when the user works on 语音转文字 (ASR / 语音识别 / Speech-to-Text) 应用工程 (从业者视角) — 选型、集成、优化语音转文字能力，尤其面向移动端、低成本、快速识别的场景。覆盖: (a) 引擎/API 地图 — 云端 API(OpenAI gpt-4o-transcribe / Whisper API、Deepgram、AssemblyAI、Google / Azure / AWS Transcribe、讯飞、字节火山、阿里、腾讯、百度) vs 开源模型(Whisper / faster-whisper / whisper.cpp / distil-whisper、NVIDIA NeMo Parakeet / Canary、阿里 FunASR / Paraformer / SenseVoice、Moonshine、Vosk、Kaldi / k2 / icefall) vs 端侧·移动 SDK(whisper.cpp + CoreML / Metal、iOS Speech framework、Android SpeechRecognizer、Picovoice、SenseVoice 端侧); (b) 准确率(WER / CER) × 延迟(RTF / 流式) × 成本 三角权衡与选型决策树; (c) 成本优化 playbook — 端侧免费 / 批量折扣 / VAD 裁静音 / 量化(int8 / ggml) / 蒸馏 / 自托管 break-even; (d) 移动端集成 — 端侧 vs 云、流式 vs 批量、断点检测(endpointing)、隐私 / 离线; (e) 后处理 — 标点 / 数字规整(ITN) / 说话人分离(diarization) / 时间戳。学派分歧: 云 API vs 端侧自托管、通用大模型(Whisper) vs 专用流式(RNN-T / Conformer)、闭源 API vs 开源、准确率派 vs 成本派、英文优先 vs 中文 ASR(FunASR / SenseVoice / 讯飞)。不含: 文字转语音(TTS / 语音合成，方向相反)、声纹识别 / 说话人验证为主业、语音 agent / 对话式 AI、ASR 模型训练科研深水区。 problems and wants industry-grade thinking, tool selection, or workflow guidance. 触发词：「语音转文字」「语音识别」「asr」「speech to text」「stt」
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Lee Cronin's Assembly Theory for molecular complexity measurement and life detection via assembly index computation.
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Streams audio from Twilio Media Streams over WebSocket to AssemblyAI real-time transcription, extracting speaker-diarized transcripts with word-level timestamps. Triggers entity detection and sentiment analysis via AssemblyAI LeMUR on completed call segments. Results are pushed to HubSpot or Salesforce contact records via their REST APIs.
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Transcribes audio and generates auto-chapters with summaries using AssemblyAI's /v2/transcript endpoint with auto_chapters=true. Extracts key topics, sentiment analysis, and content safety labels via AssemblyAI SDK.
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Extract structured intelligence from audio using the AssemblyAI API with sentiment analysis, entity detection, topic modeling, and auto-chapter generation. Uses the assemblyai Python SDK for transcript processing pipelines.
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Three-tier video pipeline: ffmpeg Quick (5s, instant) → ffmpeg Enhanced with Ken Burns (15s, polished) → Remotion Animated (60-90s, production-grade). Takes static slides from Paper MCP or any PNGs and assembles them into platform-ready video. Make sure to use this skill whenever the user has slides, images, or PNGs and wants to turn them into video — even if they just say 'make a video from these', 'animate my slides', 'I have images and need a TikTok', or 'stitch these together'. Also use when they mention ffmpeg video assembly, Remotion-animated slide assembly, Ken Burns effects, or any slides-to-video pipeline. Owns the slides→MP4 assembly pipeline; for writing, debugging, or running `remotion render` on Remotion code itself, see /remotion-best-practices; for screen-recorded product demos, see /marketing-demo. Works with any PNG source — Paper exports, Canva, screenshots, anything.
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Reverse engineers malware binaries using NSA's Ghidra disassembler and decompiler to understand internal logic, cryptographic routines, C2 protocols, and evasion techniques at the assembly and pseudo-C level. Activates for requests involving malware reverse engineering, disassembly analysis, decompilation, binary analysis, or understanding malware internals.
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End-to-end shotgun metagenomics workflow from FASTQ to taxonomic and functional profiles, orchestrating controls/host depletion, Kraken2+Bracken classification, MetaPhlAn marker profiling, and HUMAnN functional profiling. Covers the controls-first ordering, why Kraken2 read counts are not abundances and MetaPhlAn cell fractions do not equal Bracken read fractions, and the consistent-pipeline framing. Use when profiling shotgun metagenomic samples end to end, or chaining classification, abundance, and function. For resistome see metagenomics/amr-detection; for strains see metagenomics/strain-tracking; for assembly see genome-assembly/metagenome-assembly.
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Detects and removes contamination in genome assemblies via two disjoint workflows - foreign-sequence screening of a single-organism (eukaryote/isolate) assembly with NCBI FCS-GX (GenBank-submission-mandatory), FCS-adaptor, and BlobToolKit blob plots; and MAG/bin quality assessment with CheckM2 plus GUNC (chimerism) plus GTDB-Tk taxonomy, judged against MIMAG. Covers why CheckM2 alone is blind to disjoint-marker chimeras, the FCS-GX RAM wall, organelle/NUMT triage, strain heterogeneity, and the HGT-vs-contamination (tardigrade) trap. Use when screening an assembly for foreign contamination before GenBank submission, assessing MAG completeness/contamination/chimerism, deciding which contigs to remove, or distinguishing real HGT from contaminant contigs.
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Assembles haplotype-resolved diploid and telomere-to-telomere (T2T) genomes from PacBio HiFi reads with hifiasm (HiFi-only, Hi-C, or trio phasing) and verkko (HiFi + ultralong ONT for T2T), extracting contigs from GFA and routing phasing QC to k-mer/trio metrics. Covers why a primary assembly is a haplotype mosaic that exists in no cell, partial-vs-full phasing (the .bp. vs .dip. filename convention), the purge-default trap on inbred samples, the --hom-cov coverage-estimate alarm, and verkko-vs-hifiasm for T2T. Use when assembling a diploid eukaryote from HiFi, phasing haplotypes with parents (trio) or Hi-C, deciding whether to chase T2T, or diagnosing switch errors invisible to N50/BUSCO/QV.
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Assembles microbial-community sequencing into metagenome-assembled genomes (MAGs) with metaFlye (ONT), metaSPAdes/MEGAHIT (Illumina), and hifiasm-meta/metaMDBG (PacBio HiFi), then recovers genomes via multi-binner consolidation (MetaBAT2, MaxBin2, CONCOCT, SemiBin2, VAMB -> DAS_Tool) and QCs them against MIMAG with CheckM2, GUNC, and GTDB-Tk. Covers why a metagenome is not a genome (uneven coverage, micro-diversity, strain collapse to consensus), differential-coverage binning, co-assembly vs per-sample, the rRNA-operon collapse that fails short-read MAGs, and strain resolution with inStrain. Use when reconstructing genomes from a microbiome, soil, ocean, or gut community, recovering MAGs, or resolving strain-level variation.
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Assembles a genome de novo from Illumina short reads with SPAdes (isolate/careful/sc/meta/plasmid/rna modes), MEGAHIT (low-memory, huge datasets), Unicycler (bacterial finishing/hybrid), MaSuRCA (large hybrid), ABySS (Bloom-filter), and Platanus (heterozygous diploids), using multi-k de Bruijn graphs. Covers the repeat-resolution limit, why N50 plateaus at the genome not the depth, GenomeScope2 k-mer profiling first, the heterozygosity/haplotig trap, error-correction erasing rare alleles, GC dropout, and NG50/auN/BUSCO reporting. Use when assembling a bacterial isolate, fungal, small-eukaryotic, single-cell, or metagenome genome from Illumina reads, or when deciding whether short reads can even produce the assembly being asked for.
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Select, document, download, and checksum-verify the most appropriate NCBI reference genome on an HPC cluster. Use whenever an analysis needs a reference genome, genome FASTA, genome annotation, assembly accession, or genome index and no exact approved assembly is already available; when a user asks to fetch a species genome from NCBI; or when a pipeline must resolve and cache a reproducible reference assembly on a remote cluster.
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