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概要と使いどころ
ATAC-seq library quality control -- TSS enrichment, FRiP, fragment-size periodicity, library complexity (NRF/PBC1/PBC2), mitochondrial fraction, and ENCODE 4 thresholds. Use when assessing whether an ATAC-seq library passes ENCODE acceptance criteria, diagnosing transposition artefacts, comparing Omni-ATAC vs standard prep quality, or selecting which replicates to drop before peak calling.
日本語の概要は準備中です。原文の説明を表示しています。
Read, write, and convert multiple sequence alignment files using Biopython Bio.AlignIO. Supports Clustal, PHYLIP, Stockholm, FASTA, Nexus, and other alignment formats for phylogenetics and conservation analysis. Use when reading, writing, or converting alignment file formats.
日本語の概要は準備中です。原文の説明を表示しています。
Call accessible chromatin regions from ATAC-seq BAM files using MACS3, MACS2, Genrich, or HMMRATAC. Use when identifying open chromatin from aligned ATAC-seq, choosing between point-source vs HMM peak callers, applying ENCODE-style pseudoreplicate IDR, removing blacklist regions, or fixing 501bp consensus peaks for downstream differential analysis.
日本語の概要は準備中です。原文の説明を表示しています。
Predict enhancer-gene regulatory connections from ATAC-seq using ABC, ENCODE-rE2G, HiChIP, or Cicero. Use when linking distal enhancers to target genes, choosing between contact-aware (ABC, ENCODE-rE2G), accessibility-only (Cicero), and orthogonal (HiChIP H3K27ac, EpiMap) approaches, validating predictions against CRISPRi-FlowFISH gold-standard, or building cell-type-specific regulatory maps for fine-mapping or therapeutic target discovery.
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Analyzes differential transcript usage (DTU) and isoform switches with functional consequence prediction (NMD via 50nt rule, ORF disruption, protein domain loss/gain, signal peptide changes, IDR alterations, coding-potential shifts). Tools include IsoformSwitchAnalyzeR v2 (auto-selects satuRn for >5 reps else DEXSeq), the manual DRIMSeq -> DEXSeq/satuRn -> stageR DTU pipeline, and fishpond/swish for inferential-uncertainty-aware DTE. Distinguishes DTU from DGE and DTE; integrates external annotators (CPC2, Pfam, SignalP, IUPred2A or DeepTMHMM). Use when investigating how splicing differences alter protein function or trigger NMD-mediated degradation.
日本語の概要は準備中です。原文の説明を表示しています。
Run build, deploy, inspect, and fix loops for .NET MAUI apps that already have MAUI DevFlow integrated. USE FOR: launching MAUI apps, selecting devices or emulators, waiting for or recovering agent connections, broker/port/adb connectivity issues, visual tree inspection, screenshots, UI interaction, Blazor WebView CDP debugging, reading DevFlow logs, and iterative app debugging. DO NOT USE FOR: first-time DevFlow package setup (use maui-devflow-onboard), or generic desktop automation unrelated to MAUI. INVOKES: maui devflow CLI, dotnet CLI, Android adb/android tools, and Apple simctl tools.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill to evaluate which defensive driving program is best for your fleet. Compares Smith System, CarriersEdge, Compass-native training, and other options.
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Use when a carrier asks about tire program design, when to retread vs replace, position management (steer vs drive vs trailer), inflation pressure standards, casing management, scrub rates, road-call prevention, or vendor program selection (Bridgestone, Michelin, Continental, Goodyear). Covers 49 CFR 393.75 and DOT roadside out-of-service criteria.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about CMV roadside inspection levels (Level I through Level VIII), what each level inspects, average inspection times, CVSA decal eligibility, what triggers a level upgrade, and how to coach drivers through a roadside inspection. Reference CVSA North American Standard Inspection Program.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about US-Mexico cross-border CMV operations — long-haul cross-border program, B-1 visas for Mexican drivers, FMCSA OP-1(MX) authority, US-Mexico border commercial zones, USMCA / NAFTA implications, customs brokerage (C-TPAT, FAST), and how a US carrier handles cross-border loads. Reference 49 CFR 365.501-507.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about CDL driver medical certification — 49 CFR 391.41-49 medical qualifications, medical examiner's certificate (Long Form 5876 + Short Form 5875), National Registry of Certified Medical Examiners (NRCME), medical restrictions, federal disqualifying conditions, exemption programs (vision, diabetes, hearing), the FMCSA Medical Examiner's Handbook, or what to do when a driver's medical cert is downgraded. Cite specific 49 CFR 391 sections.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks how to determine whether a CMV operation is interstate vs intrastate, why it matters (federal vs state rules apply), the "continuation of journey" rule, state-specific intrastate exemptions, and what happens when an intrastate carrier picks up an interstate load. Cite 49 CFR 390.5 + state-specific exemptions.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks how to interpret a DOT medical examiner's findings — what restrictions mean (corrective lenses, hearing aid, no insulin, etc.), what conditions trigger short-term vs 24-month certificates, vision/hearing thresholds, blood pressure categories, sleep apnea screening, when to seek an exemption program, and how to handle a downgrade or denial. Cite 49 CFR 391.41-49 and the FMCSA Medical Examiner Handbook.
日本語の概要は準備中です。原文の説明を表示しています。
Use when a carrier needs to build an internal dispatcher training program, onboard new dispatchers, or certify dispatcher competency. Covers job description, required skills, training modules, certification frameworks (CDS, CTP), shadowing, evaluation, and career path.
日本語の概要は準備中です。原文の説明を表示しています。
Use when a carrier asks about fuel-card vendor selection (Comdata, EFS, WEX, RTS, Pilot Flying J, Love's Connect), discount levels, fraud control, driver authorization PINs, IFTA reporting features, cash advance, money codes, or how the card fits into payroll/settlement workflows.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when referring a driver to SAP after a positive test or refusal. Covers SAP selection, the evaluation/treatment cycle, return-to-duty test, and follow-up testing schedule.
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Use this skill when the user asks about FMCSA's Crash Preventability Determination Program (CPDP) — how to dispute that a crash was non-preventable, eligible crash types, evidence requirements, how preventability findings affect SMS scoring + safety ratings, the difference between "preventability" and "fault," and the National Crash Preventability Program. Cite FMCSA CPDP guidance.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about FMCSA Compliance Safety Accountability (CSA) program scoring — the seven BASIC categories, BASIC Severity Indicator (BSI), peer percentiles, intervention thresholds, how violations age out, SMS Methodology, ISS (Inspection Selection System), what "alert" status means, Safety Measurement System mechanics, or DataQ disputes. Cite SMS Methodology v3.20.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill to understand how FMCSA calculates the Crash Indicator BASIC. Covers what crashes count, severity weighting, time weighting, and the preventability program.
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Use this skill to use the Pre-Employment Screening Program (PSP) for evaluating driver applicants. Covers what PSP shows, cost, and how to interpret reports.
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End-to-end form handling with react-hook-form, Zod schemas, validation patterns, error messaging, field arrays, and multi-step wizards. Use for complex forms, validation architecture, autosave, field dependencies. Activate on "form validation", "react-hook-form", "Zod", "form error", "multi-step form", "wizard". NOT for simple HTML forms, backend validation only, or non-React frameworks.
日本語の概要は準備中です。原文の説明を表示しています。
Frontend stack decisions, Cloudflare deployment patterns, component systems, and internal tools architecture. Use for framework selection, deployment strategy, design system bridging, shadcn setup. Activate on "frontend architecture", "tech stack", "Cloudflare Pages", "component library", "internal tools", "shadcn setup". NOT for writing CSS (use frontend-developer), design critique (use design-critic), or backend APIs.
日本語の概要は準備中です。原文の説明を表示しています。
Secure the path from an inbound event (webhook, email, SMS, GitHub comment, file, cron) to an agent spawn in the Port Daddy fleet. Use when wiring trigger sources to agents, reviewing the io-wiring registry, adding a trust/permission gate, hardening output sinks (SSRF/path traversal), scoping tools by provenance, or proving the spawn path is safe against prompt-injection → tool-abuse. Encodes ADR-0093 and the red-team threat model. NOT for generic Coast Guard sandboxing (ADR-0050), the macaroon push-grant gate alone (ADR-0053), or non-fleet code.
日本語の概要は準備中です。原文の説明を表示しています。
Designs, reconciles, and reviews an end-to-end Drydock program for safely staging agent runtimes: immutable worktree provenance, disposable VM containment, typed effect brokerage, global body accounting, resurrection, subscription-capacity economics, context compaction, operator control, evidence, and promotion. Use when several Drydock contracts must compose into one architecture, implementation hypertree, diagram set, or release plan. NOT for launching Port Daddy, running an untrusted subject, replacing focused containment/resurrection/capacity audits, or treating a design packet as runtime authority.
日本語の概要は準備中です。原文の説明を表示しています。