644 件 ・ 関連度順
概要と使いどころ
Turn a source-bound company and market dossier into testable ideal-customer-profile hypotheses, buying roles, triggers, constraints, disqualifiers, standing, counterevidence, and research gaps. Use when a URL, company description, monitor profile, or evidence manifest needs to become defensible ICP inputs for buyer research or an AI-visibility prompt panel.
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Gate a prompt universe for schema and provenance completeness, target or campaign contamination, evidence entailment, naturalness, one-concept clarity, architecture consistency, aided status, answer leakage, and semantic duplicates. Use after realistic prompt generation and before human panel selection.
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Use when asked 帮我写开题报告, 开题答辩怎么准备, 文献综述怎么写, 技术路线图, 研究内容和创新点, or write a thesis proposal for a Chinese university. Produces a 开题报告 in the section order Chinese universities use (选题背景与意义, 国内外研究现状, 研究内容与目标, 研究方法与技术路线, 创新点, 研究基础, 进度安排, 预期成果, 参考文献), a literature review plan, a technical route diagram in text, and the questions the 开题答辩 panel is likely to ask.
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Hire an AI agent the way you'd hire an employee — a role spec with success criteria, a structured work-sample interview run on your real tasks, reference checks (what do actual users report), probation KPIs, and termination criteria written before day one. Use when choosing between AI agents/tools/copilots for a job, formalizing an AI pilot, or 'which agent should we use for X'. Produces the role spec, interview pack with scoring rubric, a decision record, and a probation plan.
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Use when the user asks the openkb chat to make a deck / slide presentation / PPT / slides / 演示稿 / 幻灯片 from their compiled KB content AND wants a dark, high-tech, neon / glow / glassmorphism look (赛博 / 科技风 / 暗色 / 霓虹 / 炫酷). Generates a polished single-file HTML deck in the Aurora Glass visual direction (near-black background, teal/sky/magenta/amber neon accents, glassmorphism panels, aurora gradient atmosphere) — opened in a browser, full-screened, shared. For the warm, printed, serif look use openkb-deck-editorial instead. Does NOT apply to generating skills (`openkb skill new`), research reports, or scrolling long-form documents.
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Produce and continuously maintain ONE living review document for a multi-item session — a cockpit header (Progress checklist, Working folder, Context) plus per-item review cards that you approve or request changes on directly in the doc or side panel. Use whenever a session has multiple deliverables you need to review/approve (meeting-processing + planning, multi-ticket work, briefs with several drafts, any "do X, then plan/draft Y and Z"). The doc is the interaction surface: you edit it (or reply in chat) to approve/change; the agent keeps it live as work progresses.
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Use when designing or auditing figures for an Advanced Materials manuscript, including the lead figure, multi-panel characterization figures, and the Table-of-Contents graphic, where visual quality is a house expectation. Designs figure strategy; does not run the analysis or write prose.
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Use when an Accounting, Organizations and Society (AOS) revise-and-resubmit arrives — planning revisions that may include recoding field data, new experimental conditions, or deepened theorizing, and drafting the point-by-point response to an interdisciplinary reviewer panel. Drafts the response; it does not run new analysis (aos-data-analysis) or the final preflight (aos-submission).
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Use when running and reporting the statistical analysis for an Academy of Management Journal (AMJ) manuscript — measurement validity, common-method bias, the right estimator (HLM, SEM, panel, experiments), endogeneity, and robustness. Executes and reports the analysis; it does not design the study (amj-methods) or frame the contribution (amj-contribution-framing).
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Render a 'model comparison grid' video from a config — a fal-style "same prompt, N contenders" showcase — a dark real-DOM stage where per beat a monospace prompt fades in centered, docks to a small top strip, then a labeled 2-4 panel grid (static images OR muted video clips, mixable per cell) staggers in and holds for comparison, plus a minimal end card — frame-stepped via Playwright (video cells are frame-seeked deterministically) and encoded with FFmpeg. Deterministic assembly, FREE (cell media comes from create-image-fal / create-video-fal, music from create-music-elevenlabs), text stays pixel-crisp. Use for the model-comparison-grid format.
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Designs pegRNAs and nicking guides for prime editing (PE) -- choosing the nick/strand, tuning the primer-binding site (PBS) and reverse-transcription template (RTT) as a per-locus panel, selecting the PE system (PE2/PE3/PE3b/PE4/PE5/PEmax/PE7), adding MMR-evading and PAM-disrupting silent edits, appending epegRNA 3' motifs (tevopreQ1/mpknot), and ranking with PRIDICT/DeepPrime. Covers twinPE/PASTE for large insertions and the prime-vs-base-editing decision. Use when designing a scarless point mutation, small insertion/deletion, or any of the 12 base conversions without a double-strand break, when efficiency is low and MMR inhibition or pegRNA stabilization is needed, or when routing a large insertion to an integrase method. Generic guide scoring and base editing are separate skills.
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Phases small variants, SVs, and methylation from Oxford Nanopore and PacBio long reads (read-backed/physical phasing) with WhatsHap, LongPhase, or HiPhase, and haplotags the BAM (HP/PS tags) for allele-resolved downstream analysis. Covers why phase blocks break at het-sparse gaps (read length x heterozygosity), why phasing the VCF is useless until the BAM is haplotagged, the GT-pipe/PS and read HP/PS tag spec, reporting block N50 with switch error, the diploid-assumption/CNV/haploid-region traps, trio phasing as the gold standard, and the boundary to statistical panel phasing. Use when phasing long-read variants, haplotagging reads for allele-specific methylation/expression or phased SVs, choosing WhatsHap vs LongPhase vs HiPhase, trio phasing, or assessing phasing quality.
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Assesses the quality and completeness of a genome annotation with BUSCO (conserved single-copy ortholog recovery), OMArk (proteome completeness, consistency, and contamination), CheckM2 (prokaryotic completeness/contamination), and a gene-set sanity panel (gene count, mono-exonic fraction, protein-length distribution, mRNA:gene ratio, coding density). Covers the assembly-BUSCO-vs-proteome-BUSCO diagnostic, what BUSCO-Duplicated really means, why gene count is a vanity metric, and the QC of transferred annotations. Use when judging whether an annotation is good enough to publish or submit, diagnosing a suspect annotation, or comparing annotation completeness across pipelines.
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Corrects fluorophore spillover (conventional compensation) or spectral overlap (spectral unmixing) and applies variance-stabilizing transforms (logicle/biexponential, arcsinh, log) for flow and mass cytometry. Covers spillover-matrix estimation from single-stain controls, AutoSpill, the spillover spreading matrix and why panel design (not compensation) bounds resolution, compensate-then-transform ordering, and arcsinh cofactor choice (5 for CyTOF, ~150 for fluorescence, per-channel via flowVS). Use when correcting spectral overlap, preparing data for gating/clustering, choosing logicle vs arcsinh, deciding a cofactor, or distinguishing compensation from spectral unmixing.
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Build UpSet plots to visualize set intersections beyond 4 sets (where Venn fails) using ComplexUpset (modern, ggplot2-grammar) or the unmaintained UpSetR, with explicit cardinality vs degree sorting, attribute panels, and query highlighting. Use when comparing overlap across many gene sets, peak sets, variant lists, or any set membership matrix where Venn diagrams become illegible.
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Creates DE-specific diagnostic and result visualizations using DESeq2/edgeR built-in functions and lightweight ggplot2 wrappers. Covers MA plot (with the shrunken-LFC compression effect), volcano (with the apeglm caveat that p-values are unchanged), PCA on VST/rlog (never raw counts), sample distance heatmaps, top-DE-gene heatmaps with the row-scaling trap, dispersion / BCV plot interpretation, p-value histogram diagnostics, plotCounts for individual genes, blind=TRUE vs FALSE rationale, and the n=3 visualization stake. Use when generating DE diagnostic plots, choosing VST vs rlog for visualization, troubleshooting suspicious plot patterns (shifted MA cloud, batch-dominated PCA, anti-conservative p-value histogram), or building a standard QC figure panel.
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Constructs and validates polygenic risk scores using LDpred2-auto, SBayesRC, MegaPRS, PRS-CS, PROSPER, MUSSEL, BridgePRS, JointPRS, PRSmix, or PGS Catalog Calculator with ancestry-aware reference panels (HapMap3, UKB-LD), ancestry-conditional calibration, and PRS-RS reporting standards. Use when computing PRS for cohorts, applying absolute-risk transformation, assessing cross-ancestry portability (Martin 2017 / Ding 2023 continuous ancestry), or auditing PRS manuscripts against the 22-item PRS-RS reviewer checklist.
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Calculates tumor mutational burden from WES/WGS/panel data with Friends of Cancer Research harmonization equations, per-assay calibration (FDA 10/Mb = 7.8 TSO500 = 8.4 OncomineTML), synonymous/indel/germline filtering, hypermutator tiering, blood TMB, and integration with HLA-LOH and neoantigen quality (Luksza 2017 fitness). Use when assessing ICI eligibility under tumor-specific cutoffs (McGrail 2021), comparing tissue vs bTMB, or auditing TMB-H reporting against ESMO 2024 and FDA pembrolizumab pan-tumor 2020.
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Batch effect correction for CRISPR screens covering ComBat empirical-Bayes, RUV, SVA, control-sgRNA normalization, and the model-based alternative of including batch as a covariate in MAGeCK MLE or Chronos. Covers screen-specific batch sources (passage cohort, library lot, infection day, sequencing run, Cas9 lot, FBS lot), PCA + variance-decomposition diagnostic to decide if correction is needed, when correction harms biology by over-correcting condition into batch, limma removeBatchEffect for visualization-only correction, and relationship to multi-condition design matrices. Use when combining screens for joint analysis, when passage cohort confounds biology, when DepMap-style panels need Chronos with batch covariates, when picking ComBat vs RUV, or when correction harms biology and should be replaced with explicit covariate modeling.
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Call copy number variants with the GATK best-practices workflows — the somatic CNV pipeline (CollectReadCounts, DenoiseReadCounts with tangent normalization, ModelSegments, CallCopyRatioSegments) and the germline GATK-gCNV pipeline (DetermineGermlineContigPloidy, GermlineCNVCaller cohort/case mode, PostprocessGermlineCNVCalls). Covers panel-of-normals construction, AnnotateIntervals/FilterIntervals, allelic-count integration, and QS-based filtering. Use when integrating CNV calling into a GATK variant pipeline, calling rare germline CNVs from an exome cohort, deciding between the somatic and germline GATK workflows, or diagnosing why tangent normalization removed a real event or why gCNV output has low precision.
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Normalize read-depth copy-ratio profiles and segment them into copy-number regions using circular binary segmentation (CBS, DNAcopy), hidden Markov models, HaarSeg, and fused-lasso methods. Covers GC-content, mappability, and replication-timing (wave-artifact) bias correction, panel-of-normals/PCA denoising, diploid-baseline centering, and algorithm selection by sequencing depth and event size. Use when choosing a segmentation algorithm, correcting depth bias, diagnosing oversegmentation or a mis-centered baseline, tuning CBS or HMM parameters, or understanding why a downstream CNV caller produced fragmented or shifted segments.
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Segments the genome into chromatin states from combinatorial histone modification and chromatin factor ChIP-seq data. Uses ChromHMM (multivariate HMM on binarized signal, v1.27), Segway (Dynamic Bayesian Network on continuous signal), EpiSegMix (flexible-distribution HMM with duration modeling, 2024), EpiLogos (multi-biosample visualization), IDEAS (cell-type-aware joint), and full-stack ChromHMM (Vu Ernst 2022) for cross-cell-type segmentations. Handles state-count selection (15 vs 18 vs 25 states), binarization choice, OverlapEnrichment / NeighborhoodEnrichment downstream analysis, and cross-biosample integration. Use when learning chromatin states from a histone mark panel, characterizing learned states by genomic feature enrichment, or comparing chromatin landscapes across cell types.
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Detects aberrant splicing in single rare-disease patients vs a control panel using FRASER 2.0 (Bioconductor; Beta-binomial autoencoder on Intron Jaccard Index, default delta cutoff 0.1, q hyperparameter), OUTRIDER (gene-level outlier expression via autoencoder denoising), LeafcutterMD (Dirichlet-multinomial outlier mode of LeafCutter for annotation-free junctions), and DROP (Snakemake pipeline integrating FRASER2 + OUTRIDER + monoallelic expression for clinical diagnostics). The statistical model is fundamentally different from differential splicing — single-sample-vs-cohort outlier detection rather than two-group comparison. Standard tool in EU rare-disease (Solve-RD) and NIH UDN programs. Use when applying RNA-seq to undiagnosed Mendelian disease, validating predicted splice variants in clinical samples, or detecting cryptic splicing in disease tissue.
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Use when building, modifying, or reviewing a Stripe App — or when the user describes something that implies one (e.g. "add a panel to the customer page", "customize my Stripe Dashboard", "react to Stripe events from my app", "connect my service to Stripe without sharing API keys"). Covers the full app development workflow (scaffold, preview, upload, versioning), UI extension architecture (sandboxed iframe, Stripe UI toolkit, viewports), extension types (UI extensions, backend-only, extension interfaces, embedded apps), authentication (platform keys, OAuth, restricted API keys), stripe-app.yaml manifest setup (permissions, viewports, CSP), webhook configuration for apps, Secret Store API, `fetchStripeSignature` auth, and marketplace publishing, plus submitting one agentic feedback report after a build. Use when the user mentions Stripe Apps, UI extensions, @stripe/ui-extension-sdk, @stripe/extensibility-sdk, script extensions, stripe-app.yaml, Dashboard extensions, or customizing the Stripe Dashboard.
日本語の概要は準備中です。原文の説明を表示しています。