「skills」の検索結果

AWS development with infrastructure automation and cloud architecture patterns

日本語の概要は準備中です。原文の説明を表示しています。

Mandatory skills, conventions, and behavioral rules for Avalonia UI development using the Zafiro toolkit.

日本語の概要は準備中です。原文の説明を表示しています。

AI-powered E2E web testing — eyes and hands for AI coding tools. Declarative YAML scenarios, Playwright execution, visual matching (OpenCV + OCR), platform auto-detection (Flutter/React/Vue), learning DB. Install: npx skills add ksgisang/awt-skill --skill awt -g

日本語の概要は準備中です。原文の説明を表示しています。

A meta-skill that understands task requirements, dynamically selects appropriate skills, tracks successful skill combinations using agent-memory-mcp, and prevents skill overuse for simple tasks.

日本語の概要は準備中です。原文の説明を表示しています。

Orchestrate multiple Antigravity skills through guided workflows for SaaS MVP delivery, security audits, AI agent builds, and browser QA.

日本語の概要は準備中です。原文の説明を表示しています。

Integracao completa com Amazon Alexa para criar skills de voz inteligentes, transformar Alexa em assistente com Claude como cerebro (projeto Auri) e integrar com AWS ecosystem (Lambda, DynamoDB, Polly, Transcribe, Lex, Smart Home).

日本語の概要は準備中です。原文の説明を表示しています。

Ingeniero de Sistemas de Andru.ia. Diseña, redacta y despliega nuevas habilidades (skills) dentro del repositorio siguiendo el Estándar de Diamante.

日本語の概要は準備中です。原文の説明を表示しています。

Meta-skill que orquestra todos os agentes do ecossistema. Scan automatico de skills, match por capacidades, coordenacao de workflows multi-skill e registry management.

日本語の概要は準備中です。原文の説明を表示しています。

Designs cytosine (CBE, C-to-T) and adenine (ABE, A-to-G) base-editor guides by positioning the target base at the activity-peak of the editing window (protospacer positions ~5-7, PAM-distal numbering), minimizing bystander edits for product purity, reading dinucleotide context (APOBEC1 TC favored / GC disfavored), and selecting the editor variant (BE4max, ABEmax, ABE8e, YE1/SECURE, TadCBE, CGBE, SpG/SpRY-BE). Covers knockout by premature stop (CRISPR-STOP/iSTOP) and splice-site disruption, the three off-target classes (Cas-dependent, Cas-independent DNA, RNA), outcome prediction (BE-Hive/DeepBE), and the base-vs-prime-vs-HDR decision. Use when installing a transition mutation without a double-strand break, knocking out a gene without indels, or choosing CBE vs ABE. Generic guide scoring, prime editing, and HDR donors are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。

Designs and ranks guide RNAs (sgRNAs) for CRISPR-Cas9/Cas12a gene knockout by scanning a target for PAM sites (NGG SpCas9, NNGRRT SaCas9, TTTV Cas12a, NG SpCas9-NG, near-PAMless SpRY), enumerating candidate spacers, applying hard filters (Pol-III TTTT terminator, 5' G, GC), ranking on-target activity with the context-appropriate model (Rule Set 2/Azimuth for U6/lentiviral, CRISPRscan for T7/embryo, DeepHF for high-fidelity variants, DeepCpf1 for Cas12a), and predicting the indel/frameshift outcome (Bae out-of-frame score, inDelphi, FORECasT, Lindel). Use when selecting sgRNAs to knock out a gene, choosing a nuclease/PAM for a constrained locus, picking which exon to target, or shortlisting guides before an off-target check. Off-target specificity, base/prime editing, and HDR donors are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。

Designs donor/repair templates for precise CRISPR knock-ins -- choosing the format (ssODN, long-ssDNA/Easi-CRISPR, dsDNA/plasmid, AAV6), sizing homology arms, placing the cut within ~10 bp of the edit, and adding a mandatory codon-checked blocking (PAM/seed) mutation so the edited allele is not re-cut. Frames the HDR-vs-NHEJ-vs-MMEJ pathway competition, the MMEJ (PITCh) and homology-independent (HITI/HMEJ) alternatives for post-mitotic cells, ssODN strand/asymmetry choice, phosphorothioate end-protection, and ranked HDR enhancers. Use when designing a donor for a point mutation, epitope/fluorophore tag, allele replacement, or knock-in, or when HDR efficiency is low. Guide design and base/prime editing are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。

Nominates and assesses CRISPR off-target sites genome-wide. Enumerates candidate sites by mismatch and bulge tolerance with Cas-OFFinder/CRISPRitz, ranks them with the published CFD score (SpCas9-only, relative ranker) or MIT/CRISTA/energy models, runs variant-aware screening against gnomAD/individual genomes (CRISPRme), and frames the empirical genome-wide discovery assays (GUIDE-seq, CIRCLE-seq, CHANGE-seq, DISCOVER-seq, Digenome-seq) and high-fidelity nuclease choice (HiFi Cas9, Sniper-Cas9, eSpCas9, SpCas9-HF1). Use when assessing guide RNA specificity, choosing among candidate guides, screening a therapeutic guide against population variation, or planning empirical off-target validation. Distinguishes predicted vs detected vs validated. On-target activity scoring and deaminase (Cas-independent) base/prime-editor off-targets are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。

Designs pegRNAs and nicking guides for prime editing (PE) -- choosing the nick/strand, tuning the primer-binding site (PBS) and reverse-transcription template (RTT) as a per-locus panel, selecting the PE system (PE2/PE3/PE3b/PE4/PE5/PEmax/PE7), adding MMR-evading and PAM-disrupting silent edits, appending epegRNA 3' motifs (tevopreQ1/mpknot), and ranking with PRIDICT/DeepPrime. Covers twinPE/PASTE for large insertions and the prime-vs-base-editing decision. Use when designing a scarless point mutation, small insertion/deletion, or any of the 12 base conversions without a double-strand break, when efficiency is low and MMR inhibition or pegRNA stabilization is needed, or when routing a large insertion to an integrase method. Generic guide scoring and base editing are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。

Add MAUI DevFlow to a .NET MAUI project with agent package references, MauiProgram.cs registration, Blazor WebView support, GTK variants, Central Package Management guidance, and verification commands. USE FOR: first-time DevFlow setup, reviewing what files to edit, choosing DevFlow packages, or continuing after `maui devflow init` installs skills. DO NOT USE FOR: troubleshooting an already-integrated app that cannot connect, iterative app debugging, UI inspection, or generic MAUI build failures (use maui-devflow-debug). INVOKES: maui devflow CLI and dotnet CLI.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill to develop the capability to investigate crashes internally. Covers what to look for, when to escalate to outside experts, and documentation standards.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when selecting a TPR-registered Training Provider for behind-the-wheel ELDT training. Covers the registry, evaluation criteria, and common pitfalls.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill to evaluate document retention software options. Covers compliance features, ease of use, integration, and cost.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when documenting supervisor reasonable-suspicion training for Part 382 compliance. Covers what to log, what to retain, and what training providers to use.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill to understand FMCSA tire tread depth requirements. Covers steer tires (4/32 minimum) and other axles (2/32 minimum), and how to measure correctly.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill to understand trailer-specific inspection requirements when a trailer is not coupled to a tractor. Covers annual inspection, DVIR rules, and parking inspections.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks about state-specific trucking taxes BEYOND IFTA — NY Highway Use Tax (HUT), KY Kentucky Intrastate Tax (KIT), Oregon Weight-Mile Tax, New Mexico Weight-Distance Tax, Connecticut Highway Use Fee, or other state-level road-use taxes for commercial vehicles. Covers thresholds, rates, registration, filing cadence, and how these differ from IFTA. Reference state DOT/DOR resources.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks about trucking rate negotiation — cost-plus pricing, market rate benchmarking via DAT/Truckstop, fuel surcharge math, how to negotiate with brokers/shippers, when to walk away, contract pricing vs spot rates. Reference DAT Rate Analytics + freight market reports.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill to understand when electronic records are acceptable to FMCSA inspectors. Covers PDF, original-format, and search-ability requirements.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks about CMV roadside inspection levels (Level I through Level VIII), what each level inspects, average inspection times, CVSA decal eligibility, what triggers a level upgrade, and how to coach drivers through a roadside inspection. Reference CVSA North American Standard Inspection Program.

日本語の概要は準備中です。原文の説明を表示しています。