「gpt」の検索結果

Comprehensive SEO analysis for any website or business type. Full site audits, single-page analysis, technical SEO (crawlability, indexability, Core Web Vitals with INP), schema markup, content quality (E-E-A-T), image optimization, sitemap analysis, and GEO for AI Overviews/ChatGPT/Perplexity. Industry detection for SaaS, e-commerce, local, publishers, agencies. Triggers on: SEO, audit, schema, Core Web Vitals, sitemap, E-E-A-T, AI Overviews, GEO, technical SEO, content quality, page speed.

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Strip the machine fingerprint out of any draft - em dashes, AI slop words, invisible watermark characters - and score it against a five-check detection panel before it goes out. Use whenever text needs to sound human, when the user says humanize, "does this sound like AI", "remove the em dashes", "de-slop this", "this sounds like ChatGPT", or before any caption, script, comment, reply or DM is shown to the user.

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Check and improve your brand's visibility across AI search engines (ChatGPT, Perplexity, Gemini, Grok, Claude, DeepSeek). Set up tracking, run visibility analyses, audit your website for AI readability, and get actionable recommendations. Uses the npx goose-aeo@latest CLI.

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the UGC fix-loop toolkit — surgically re-render a bad window/beat of a single-take UGC master (stitch_replacement.py, pure FFmpeg) and GPT cross-model review a Seedance prompt before render (vet_seedance_prompt.py, routed through the openai-proxy). Fetch it into a one-shot UGC recipe so both scripts resolve on any machine and the vet call bills the Ads agent.

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Assemble a stop-motion hand-swatch-cycle product-demo ad from a config — a sequence of still PLATES (one hand swiping a single-barrel product across a test surface, the barrel + swatch changing per plate while the hand, background, crop, and lighting stay locked) is PNG→mp4 loop-encoded at each plate's own stop-motion hold (fast motion frames 150–250ms, per-shade ~380ms, hero beats 1100–1800ms), concat-demuxed with HARD cuts into a silent master, closed on a Playwright HTML-rendered branded end card (serif tagline + sans subtitle + real logo SVG over a hero BG, never AI-rendered text), and muxed with a pre-sourced music track playing under the end card with a fade tail (no VO). This is the FREE deterministic assembly stage (loop-encode + concat-demux + end-card render + music mux); the master-anchor plate, shade plates, and end-card BG come from create-image-gpt-image-fal and the track from create-music-elevenlabs. Use for the stopmotion-hand-swatch-cycle format.

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Assemble an Apple Notes list video ad from a note + end-card JSON — a frame-accurate fake iPhone screen recording of a short list being typed into Apple Notes (character by character, key pops, blinking caret, the note scrolling up as it fills) crossfaded into a checklist end card (the note's picks as ticked rows, real product photos, logo, CTA, in the brand's colours and fonts), with an optional music bed. FREE assembly (Playwright + ffmpeg); the recipe supplies the per-brand note + end card and gates the paid music call to its own capability. The Apple Notes sibling of render-imessage-chat and render-chatgpt-chat. Use for the apple-notes format.

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Assemble a cinematic live-action-style music-video ad from a config — an original sung anthem carries the whole narrative while N 35mm-film-look i2v clips are each cut to their lyric window and hard-concatenated on the beat as a 3-act arc, the anthem muxed at loudnorm I=-14, cinematic lower-third serif captions built from the song's OWN word timings (never Whisper) with the hook line landing on the chorus drop, and closed on a brand end card composited from the real asset — never AI-rendered text. This is the FREE deterministic assembly stage (cut-to-window + hard concat + anthem mux + captions + end card); the anthem, keyframes, and clips come from create-music-elevenlabs / create-image-gpt-image-fal / create-video-fal. Use for the cinematic-music-video format.

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Assemble a multi-scene GRWM beauty-demo ad from a config — a locked-identity creator applies ~5 products step by step while a SEPARATE ElevenLabs voiceover narrates and every scene cut is snapped to the VO's product-name word-starts (Whisper word-level timestamps), then ~5 Playwright product overlay cards (real PDP-verified taglines) are composited onto the master each on its product-NAME word-start, the SEPARATE VO is mixed on top of a ducked music bed at loudnorm I=-14, clean-white 3-words/cue captions are burned, and the video closes on a flat-lay end card. This is the FREE deterministic assembly stage (re-cut to the VO word-starts, hard-concat, Playwright card render + card composite, VO plus music mix, caption burn, flat-lay end card); the VO, scene clips, product cutouts, and music come from create-music-elevenlabs / create-image-gpt-image-fal / create-video-fal. Use for the glassy-matte-grwm format.

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Assemble a split-screen creator ad from a config — a two-zone vertical composite where a supplied AI-creator lip-sync take fills the BOTTOM ~48% while real 16:9 product/demo clips run uncropped in the TOP ~52%, each top clip contain-fit with a darkened blurred cover-scale fill of the same clip (never black bars), a 3px brand-color divider between the zones, the creator slice cover-fit per the per-scene VO timing, scenes hard-concatenated with the body audio being the concatenated creator VO slices, an end card held on the last sharp frame ~3s, then the ASSEMBLED cut transcribed with local Whisper (not the raw VO — concat drops inter-scene silence) and word-level captions burned in the chosen style. This is the FREE deterministic assembly + caption stage (two-zone composite + blurred fill + divider + hard-concat + end card + captions); the VO comes from create-vo-elevenlabs, the anchor from create-image-gpt-image-fal, and the whole-VO lip-sync from a paid VEED Fabric 1.0 take (a no-atom upstream input). Use for the split-screen-creator format.

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Assemble a narrated-UGC "stitch reply" ad from a config — a single spoken VO carries a verbatim testimonial while ~30 per-cut i2v clips (one creator across ~5 wardrobes in ~3 worlds, plus product B-roll) are each trimmed to their EDL window built from the VO's Whisper word boundaries and hard-concatenated via filter_complex concat (never the demuxer, which drops audio on a duration mismatch), the VO mixed over an optional sidechain-ducked instrumental bed (−20dB, 20 to 1) so the VO stays on top, karaoke-pop captions burned on every word throughout (VEED Whisper preset, re-spelled against the locked script), a landing-page scroll rendered as FFmpeg zoompan over a Playwright PNG (not i2v), and closed on the brand's real end-card PNG — never AI-rendered text. This is the FREE deterministic assembly stage (trim-to-EDL + filter_complex concat + VO and music mix + karaoke captions + landing-page zoompan + end-card append), run with the shared stitch-videos-ffmpeg montage.py helper that installs with this package; the VO, creator, start-frames, and clips come from create-vo-elevenlabs / create-image-gpt-image-fal / create-image-fal / create-video-fal. Use for the narrated-ugc-wardrobe-stitch format.

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Tracks ctDNA across serial liquid-biopsy timepoints for molecular residual disease (MRD) and treatment-response monitoring, treating MRD as a binary integrated detection call across the patient's full variant set (with a defined LoD95 and per-sample specificity) rather than a per-timepoint VAF threshold, and handling undetectable samples as left-censored at the per-sample limit of detection rather than true zeros. Covers tumor-informed bespoke vs tumor-naive design, landmark vs surveillance sampling, molecular-response definitions and their non-standardization, censoring-aware clearance kinetics, and the multiple-testing structure of repeated surveillance. Use when monitoring ctDNA during therapy, calling molecular relapse before imaging, or estimating clearance half-life from serial samples.

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Detects TAD boundaries from balanced Hi-C contact matrices via the diamond-window insulation score (cooltools insulation) and HiCExplorer hicFindTADs, returning a continuous log2 insulation track, valley-prominence boundary_strength, and Li/Otsu-thresholded is_boundary flags across a list of window sizes. Covers the multi-scale window sweep (sub-TAD to compartment-domain), why the boundary is reproducible but the domain partition is not, cross-condition comparison via differential SCORE not differential partition, and the insulation-vs-compartment orthogonality. Use when calling TADs or domain boundaries, computing insulation scores, choosing a window size, ranking boundary strength, comparing boundaries across conditions, or annotating CTCF-backed boundaries; route domain rendering to hic-visualization and boundary-feature overlap to genome-intervals.

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Balances Hi-C contact matrices (ICE via cooler.balance_cooler, KR/SCALE/VC context), computes distance-decay expected with cooltools (expected_cis per-diagonal P(s), expected_trans scalar), builds observed/expected (O/E) matrices, and diagnoses polymer state from the P(s) log-derivative. Covers the within-matrix-vs-cross-sample distinction (balancing is NOT a normalizer), the equal-visibility assumption that CNV/aneuploidy violates (use raw counts for copy-number), cis-only balancing, mad_max/blacklist masking before balancing, multiplicative cooler weights vs divisive juicer weights, and the resolution-vs-depth budget. Use when balancing a .cool/.mcool, computing expected or P(s), making O/E matrices for compartments/loops, deciding ICE vs KR vs SCALE, choosing a resolution for a given depth, or troubleshooting NaN/all-NaN balanced matrices; route cross-sample comparison to hic-differential.

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Detects cancer and infers tissue-of-origin from cfDNA methylation by choosing conversion chemistry (bisulfite vs EM-seq vs TAPS vs cfMeDIP), calling read-level methylation haplotypes rather than averaged beta values, and deconvolving a hematopoietic-dominated cfDNA mixture against a methylation atlas via NNLS/quadratic programming. Encodes the GRAIL/CCGA thesis that thousands of tissue-specific markers make methylation outperform sparse mutations for multi-cancer early detection (MCED) and localization, and that single concordantly-methylated fragments give ppm-level sensitivity. Uses MethylDackel for extraction (mbias-then-extract), MEDIPS/QSEA for enrichment data, scipy.optimize.nnls for deconvolution. Use when building an MCED or methylation-MRD assay, picking a conversion chemistry for low-input plasma, or deconvolving tissue-of-origin from cfDNA.

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Load and preprocess imaging mass cytometry (IMC) and MIBI data from raw MCD/TXT through hot-pixel removal, spillover compensation, and variance-stabilizing transformation, covering readimc/steinbock ingestion, NNLS spillover compensation (CATALYST), IMC-Denoise, and the IMC arcsinh-cofactor question. Use when starting analysis from raw MCD files, building per-channel TIFF stacks, compensating channel spillover, choosing an arcsinh cofactor, or preparing single-cell intensities for phenotyping.

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Detects focal chromatin loops (point interactions / corner-dots) in balanced Hi-C and Micro-C contact maps and aggregates/validates a loop set. Covers de-novo calling with cooltools dots (HiCCUPS-style 4-background local enrichment with lambda-chunked FDR), chromosight (template-correlation), and Mustache (scale-space blob detection); aggregate peak analysis (APA) via cooltools pileup for confirmation; the depth/resolution prerequisite (de-novo needs ~5-10kb resolution = hundreds of millions to billions of valid pairs); consensus across callers and convergent-CTCF support as validation; and differential loops via union anchors plus chromosight quantify. Use when calling chromatin loops or dots from a cooler, deciding whether a map is deep enough to call de-novo vs running APA on known CTCF/cohesin anchors, building an aggregate peak pileup, comparing loops across conditions, or validating loop calls. For HiChIP/PLAC-seq/PCHi-C protein-anchored data use FitHiChIP/MAPS, not dots.

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Detects somatic mutations in circulating tumor DNA, treating low-VAF detection as a signal-versus-noise problem set by error suppression and molecules sampled, not by the choice of caller. Distinguishes de novo CALLING (scanning a panel for unknown variants, bounded by per-locus error and multiple testing) from tumor-informed DETECTION (tracking a pre-specified variant set, where panel integration reaches single-ppm). Covers VarDict and Mutect2 for de novo calling, UMI-aware callers, and a pysam-based known-variant VAF tracker, with matched-WBC subtraction as the mandatory defense against clonal hematopoiesis (the dominant false positive). Use when calling or tracking tumor mutations from plasma cfDNA, setting a VAF threshold, or deciding whether a low-VAF call is tumor versus CHIP.

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Assign cell types from marker expression in IMC/MIBI data using clustering (PhenoGraph/FlowSOM/Leiden/Pixie), marker-based probabilistic classifiers (Astir), or image-context CNNs (CellSighter), covering the double-positive segmentation artifact, lineage-vs-state markers, the two spillover types, and why a "cell type" in imaging is conditioned on a segmentation guess. Use when phenotyping segmented IMC cells, choosing clustering vs classification, diagnosing implausible double-positive populations, separating lineage from functional markers, or transferring labels across a cohort.

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Interactive cell annotation and image QC for IMC/MIBI using napari, napari-imc, Mantis Viewer, and cytomapper, covering the pixels-to-cell-table bridge, overlaying masks to catch segmentation/spillover artifacts, inter-annotator variability as the accuracy ceiling, contrast-as-threshold, and building class-balanced ground-truth label sets. Use when manually labeling cells, generating training data for a classifier, QC-ing segmentation on the image, confirming clusters are spatially real, or choosing an annotation viewer.

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Predict B-cell and T-cell epitopes for vaccine antigen design and epitope mapping with BepiPred-3.0, DiscoTope-3.0, the IEDB tools, and EL-mode MHC presentation. Encodes the load-bearing asymmetry that T-cell epitope prediction is mature (it reduces to MHC presentation, AUC>0.9) while B-cell prediction is unreliable (linear predictors ~AUC 0.6 because ~90% of real epitopes are conformational) — so structure-based DiscoTope-3.0 on AlphaFold models is the only defensible B-cell path, propensity scales are obsolete, and NetChop is largely redundant on EL-trained models. Use when mapping epitopes or selecting vaccine antigens. MHC binding lives in mhc-binding-prediction.

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Calls germline small variants (SNPs and indels) from Oxford Nanopore and PacBio HiFi long reads with Clair3, a two-stage (pileup + full-alignment) deep-learning caller, selecting the chemistry- and basecaller-version-matched model, enabling read-based phasing, and benchmarking against GIAB with stratification. Covers why the model string is the experiment (no auto-detection, silent degradation on mismatch), why ONT homopolymer/STR indels are the residual error whole-genome F1 hides, and the somatic/trio/RNA boundary to the ClairS/Clair3-Trio family. Use when calling germline SNVs/indels from ONT or HiFi BAMs, choosing a Clair3 model, phasing variants, or benchmarking long-read calls.

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Estimates tumor fraction (the genome-wide proportion of cfDNA molecules that are tumor-derived, the cfDNA analogue of bulk-tumor purity) from shallow whole-genome sequencing with ichorCNA, an HMM over 1 Mb bins that jointly EM-estimates tumor fraction, ploidy, and subclonal prevalence over a normal/ploidy grid. Encodes the load-bearing reframes: tumor fraction is the quantity that travels across assays and is NOT mutation VAF (clonal-het VAF approximately TF/2), CNA-based estimation has a hard ~3 percent limit-of-detection floor, and near-diploid or copy-neutral-LOH genomes return a falsely low value. Selects the estimator by data type (sWGS to ichorCNA, deep panel to max-VAF, methylation to deconvolution, sub-3 percent to fragmentomics or methylation). Use when quantifying tumor burden from a liquid biopsy, picking a tumor-fraction estimator for a given assay, or reconciling a TF estimate against a panel VAF.

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Analyze spatial cell-cell interactions, neighborhoods, and niches in IMC/MIBI data with squidpy and imcRtools, covering neighborhood-enrichment permutation nulls, the abundance-vs-density confound, inhomogeneous Ripley's K, cellular-neighborhood discovery, graph-construction (contact vs proximity), and edge effects. Use when testing whether cell types co-locate, choosing a spatial null, building a neighbor graph, discovering tissue niches, or deciding whether a spatial pattern is real or a density/segmentation artifact.

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Compare cell-type composition and spatial features across conditions in IMC/MIBI cohorts with the patient as the experimental unit, covering pseudoreplication, per-patient aggregation, mixed models, compositional (Dirichlet/scCODA) differential abundance, diffcyt, per-image-to-patient spatial differential testing (SpaceANOVA), batch covariates, and FDR. Use when testing whether a cell type or spatial niche differs between groups, avoiding cell-level pseudoreplication, choosing a differential-abundance method, or correctly powering an IMC cohort comparison.

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