「sem」の検索結果

Orchestrates an end-to-end de novo genome assembly project, routing each step to the right genome-assembly skill rather than restating it. Profiles the genome first (k-mer spectrum -> size, heterozygosity, ploidy), QCs reads, chooses an assembly path by data type (SPAdes for Illumina, Flye for noisy long reads, hifiasm for HiFi, metaFlye for communities), polishes only when needed, decontaminates, scaffolds with Hi-C, and finishes with three-axis QC (contiguity + completeness + correctness). Use when assembling a genome from raw reads and deciding which assembler, whether to polish, and how to prove the result is good.

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Assembles microbial-community sequencing into metagenome-assembled genomes (MAGs) with metaFlye (ONT), metaSPAdes/MEGAHIT (Illumina), and hifiasm-meta/metaMDBG (PacBio HiFi), then recovers genomes via multi-binner consolidation (MetaBAT2, MaxBin2, CONCOCT, SemiBin2, VAMB -> DAS_Tool) and QCs them against MIMAG with CheckM2, GUNC, and GTDB-Tk. Covers why a metagenome is not a genome (uneven coverage, micro-diversity, strain collapse to consensus), differential-coverage binning, co-assembly vs per-sample, the rRNA-operon collapse that fails short-read MAGs, and strain resolution with inStrain. Use when reconstructing genomes from a microbiome, soil, ocean, or gut community, recovering MAGs, or resolving strain-level variation.

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Maps between gene identifier systems (Ensembl, Entrez, HGNC symbol, UniProt, RefSeq, MANE) using AnnotationDbi, biomaRt, mygene, pyensembl, and Ensembl REST. Encodes Ensembl version stripping with GENCODE _PAR_Y preservation, the Ziemann 2016 Excel autocorrect debacle and Bruford 2020 HGNC renames (SEPT*->SEPTIN*, MARCH*->MARCHF*, MARC*->MTARC*, DEC1->DELEC1), OCT4/POU5F1 alias resolution, biomaRt archive endpoints for release pinning, the `filters` (plural) gotcha, MANE Select for clinical reporting, cross-species orthology via Ensembl Compara / OMA / OrthoDB, and tx2gene construction for tximport. Use when converting gene IDs across systems, handling renamed symbols, building tx2gene, pinning to a specific Ensembl release for reproducibility, or mapping cross-species orthologs.

日本語の概要は準備中です。原文の説明を表示しています。

Query the Ensembl REST API for gene/transcript/protein lookup, sequence retrieval, comparative genomics (Compara), variant effect prediction (VEP), regulatory features, and cross-species ortholog/paralog calls. Use when pulling Ensembl-native data (Ensembl Gene IDs, version-pinned releases, archive endpoints for reproducibility), gene/transcript/exon structure with stable IDs, or VEP for variant annotation. Encodes the 15 req/sec rate limit, archive (e110.rest.ensembl.org) for reproducibility, Ensembl divisions (vertebrates / plants / fungi / metazoa / bacteria), and the symbol-vs-ID stability problem.

日本語の概要は準備中です。原文の説明を表示しています。

Assembles microbial-community sequencing into metagenome-assembled genomes (MAGs) with metaFlye (ONT), metaSPAdes/MEGAHIT (Illumina), and hifiasm-meta/metaMDBG (PacBio HiFi), then recovers genomes via multi-binner consolidation (MetaBAT2, MaxBin2, CONCOCT, SemiBin2, VAMB -> DAS_Tool) and QCs them against MIMAG with CheckM2, GUNC, and GTDB-Tk. Covers why a metagenome is not a genome (uneven coverage, micro-diversity, strain collapse to consensus), differential-coverage binning, co-assembly vs per-sample, the rRNA-operon collapse that fails short-read MAGs, and strain resolution with inStrain. Use when reconstructing genomes from a microbiome, soil, ocean, or gut community, recovering MAGs, or resolving strain-level variation.

日本語の概要は準備中です。原文の説明を表示しています。

Maps between gene identifier systems (Ensembl, Entrez, HGNC symbol, UniProt, RefSeq, MANE) using AnnotationDbi, biomaRt, mygene, pyensembl, and Ensembl REST. Encodes Ensembl version stripping with GENCODE _PAR_Y preservation, the Ziemann 2016 Excel autocorrect debacle and Bruford 2020 HGNC renames (SEPT*->SEPTIN*, MARCH*->MARCHF*, MARC*->MTARC*, DEC1->DELEC1), OCT4/POU5F1 alias resolution, biomaRt archive endpoints for release pinning, the `filters` (plural) gotcha, MANE Select for clinical reporting, cross-species orthology via Ensembl Compara / OMA / OrthoDB, and tx2gene construction for tximport. Use when converting gene IDs across systems, handling renamed symbols, building tx2gene, pinning to a specific Ensembl release for reproducibility, or mapping cross-species orthologs.

日本語の概要は準備中です。原文の説明を表示しています。

Query the Ensembl REST API for gene/transcript/protein lookup, sequence retrieval, comparative genomics (Compara), variant effect prediction (VEP), regulatory features, and cross-species ortholog/paralog calls. Use when pulling Ensembl-native data (Ensembl Gene IDs, version-pinned releases, archive endpoints for reproducibility), gene/transcript/exon structure with stable IDs, or VEP for variant annotation. Encodes the 15 req/sec rate limit, archive (e110.rest.ensembl.org) for reproducibility, Ensembl divisions (vertebrates / plants / fungi / metazoa / bacteria), and the symbol-vs-ID stability problem.

日本語の概要は準備中です。原文の説明を表示しています。

Analyze and design an AssemblyAI architecture spanning pre-recorded jobs, Streaming v3, LLM Gateway, callbacks, queues, retention, and audit evidence. Use when performing system design or review. Trigger with "AssemblyAI architecture" or "design AssemblyAI pipeline".

日本語の概要は準備中です。原文の説明を表示しています。

Analyze and operate an AssemblyAI Streaming v3 session with safe tokens, audio framing, turn handling, and explicit termination. Use when building live captions or voice agents. Trigger with "AssemblyAI streaming", "AssemblyAI v3 WebSocket", or "live transcript".

日本語の概要は準備中です。原文の説明を表示しています。

Analyze and apply AssemblyAI secret, region, consent, retention, deletion, and model-training controls. Use when reviewing security or regulated speech workflows. Trigger with "AssemblyAI security", "AssemblyAI privacy", or "data retention".

日本語の概要は準備中です。原文の説明を表示しています。

Analyze and process AssemblyAI pre-recorded and streaming webhooks with authentication, fast acknowledgment, deduplication, and safe retrieval. Use when implementing event-driven completion. Trigger with "AssemblyAI webhook" or "AssemblyAI callback".

日本語の概要は準備中です。原文の説明を表示しています。

Deploy AssemblyAI workers, Streaming v3 token services, and authenticated callback receivers with reversible controls. Use when shipping cloud infrastructure. Trigger with "deploy AssemblyAI" or "AssemblyAI webhook deployment".

日本語の概要は準備中です。原文の説明を表示しています。

Analyze and tune AssemblyAI model choice, audio delivery, polling, Streaming v3 turns, concurrency, and downstream work. Use when pursuing measured latency or throughput goals. Trigger with "tune AssemblyAI" or "AssemblyAI latency".

日本語の概要は準備中です。原文の説明を表示しています。

Build an AssemblyAI local loop with synthetic fixtures, recorded contracts, and an optional bounded live lane. Use when developing without exposing audio or uncontrolled credits. Trigger with "AssemblyAI local dev" or "mock AssemblyAI".

日本語の概要は準備中です。原文の説明を表示しています。

PCBWay PCB fabrication and assembly — turnkey/consigned assembly, design rules, ordering workflow. Alternative to JLCPCB for manufacturing. Use with KiCad. Use this skill when the user mentions PCBWay, needs turnkey assembly (PCBWay sources parts by MPN), has parts not available on LCSC, needs assembled boards with non-LCSC components, wants to compare PCBWay vs JLCPCB, or needs assembly with parts sourced globally rather than from LCSC only. For gerber/CPL export, stencil ordering, and BOM management, see the `bom` skill.

日本語の概要は準備中です。原文の説明を表示しています。

PCBWay PCB fabrication and assembly — turnkey/consigned assembly, design rules, ordering workflow. Alternative to JLCPCB for manufacturing. Use with KiCad. Use this skill when the user mentions PCBWay, needs turnkey assembly (PCBWay sources parts by MPN), has parts not available on LCSC, needs assembled boards with non-LCSC components, wants to compare PCBWay vs JLCPCB, or needs assembly with parts sourced globally rather than from LCSC only. For gerber/CPL export, stencil ordering, and BOM management, see the `bom` skill.

日本語の概要は準備中です。原文の説明を表示しています。

Assembles a genome de novo from Illumina short reads with SPAdes (isolate/careful/sc/meta/plasmid/rna modes), MEGAHIT (low-memory, huge datasets), Unicycler (bacterial finishing/hybrid), MaSuRCA (large hybrid), ABySS (Bloom-filter), and Platanus (heterozygous diploids), using multi-k de Bruijn graphs. Covers the repeat-resolution limit, why N50 plateaus at the genome not the depth, GenomeScope2 k-mer profiling first, the heterozygosity/haplotig trap, error-correction erasing rare alleles, GC dropout, and NG50/auN/BUSCO reporting. Use when assembling a bacterial isolate, fungal, small-eukaryotic, single-cell, or metagenome genome from Illumina reads, or when deciding whether short reads can even produce the assembly being asked for.

日本語の概要は準備中です。原文の説明を表示しています。

Decides whether and how to polish a draft genome assembly to raise consensus accuracy (QV) with read-type-matched tools - Racon and medaka (ONT consensus), dorado polish, Polypolish and pypolca (Illumina, repeat-aware), Pilon (legacy short-read), NextPolish/NextPolish2, Hapo-G (haplotype-aware), ntEdit, and DeepPolisher/PEPPER-Margin-DeepVariant for human. Covers the do-not-polish-HiFi rule, the medaka basecaller-model footgun, held-out Merqury QV as the only honest stop signal, and the haplotype-collapse trap. Use when correcting homopolymer indels or residual SNPs in a long-read assembly, deciding if a HiFi assembly needs polishing, or choosing an ONT vs hybrid vs short-read polishing chain.

日本語の概要は準備中です。原文の説明を表示しています。

Assembles haplotype-resolved diploid and telomere-to-telomere (T2T) genomes from PacBio HiFi reads with hifiasm (HiFi-only, Hi-C, or trio phasing) and verkko (HiFi + ultralong ONT for T2T), extracting contigs from GFA and routing phasing QC to k-mer/trio metrics. Covers why a primary assembly is a haplotype mosaic that exists in no cell, partial-vs-full phasing (the .bp. vs .dip. filename convention), the purge-default trap on inbred samples, the --hom-cov coverage-estimate alarm, and verkko-vs-hifiasm for T2T. Use when assembling a diploid eukaryote from HiFi, phasing haplotypes with parents (trio) or Hi-C, deciding whether to chase T2T, or diagnosing switch errors invisible to N50/BUSCO/QV.

日本語の概要は準備中です。原文の説明を表示しています。

Fits structural equation models to GWAS summary statistics using GenomicSEM (Grotzinger 2019), including common-factor models, confirmatory factor models, ESEM, common-factor GWAS with Q_SNP heterogeneity, multivariate Wald tests, and stratified GenomicSEM partitioned heritability. Reconciles results against MTAG multi-trait analysis. Handles sample overlap via the LDSC sampling-covariance matrix, identifies and resolves Heywood cases, and verifies model fit with CFI / RMSEA. Use when modeling latent genetic architecture across correlated traits, running multivariate GWAS on a shared factor, distinguishing factor-mediated from trait-specific SNP effects, or comparing GenomicSEM common-factor results against MTAG when both depend on accurate sampling covariance.

日本語の概要は準備中です。原文の説明を表示しています。

Provides sem semantic-diff detection, install-on-first-use, and fallback patterns. Use when building skills that consume git diff output.

日本語の概要は準備中です。原文の説明を表示しています。

Polish genome assemblies to reduce errors using short reads (Pilon), long reads (Racon), or ONT-specific tools (medaka). Essential for improving long-read assembly accuracy. Use when improving assembly accuracy with polishing tools.

日本語の概要は準備中です。原文の説明を表示しています。

Fix silent failures when parsing Grafema semantic IDs that are in URI format instead of legacy arrow format. Use when: (1) code splits semantic IDs by "->" but gets the whole string back because IDs are grafema:// URIs, (2) file path extraction from semantic IDs returns empty or wrong values, (3) derived edges (DEPENDS_ON, etc.) produce 0 results despite source edges existing, (4) any code that processes semantic IDs after the analysis pipeline's to_uri_format() has run. The grafema:// URI format uses # fragments with percent-encoded characters instead of -> separators.

日本語の概要は準備中です。原文の説明を表示しています。

Assembly programming and low-level debugging skill for reading, writing, explaining, optimizing, and reviewing assembly across x86, x86-64, ARM, AArch64, RISC-V, calling conventions, ABI boundaries, inline assembly, disassembly, stack frames, registers, and binary-level behavior. Use for .s/.asm files, compiler output, reverse-engineering snippets, low-level performance, and crash analysis.

日本語の概要は準備中です。原文の説明を表示しています。