「rca」の検索結果

Cria, atualiza, comenta, fecha e reabre issues do GitHub e gerencia as tarefas de um GitHub Project — mover Status, editar campos, sub-issues e dependências "blocked by", inclusive em lote. Use quando o usuário disser "criar issue", "abrir uma issue", "fechar a issue #12", "reabrir", "comentar na issue", "atribuir para", "mover para Em andamento", "marcar como concluída", "bloqueada por", "sub-issue", "o que está bloqueando", "tarefas do project", "fechar as tasks da feature", ou citar #123 num repo GitHub. Não use para criar um Project novo (skill github-project) nem para publicar um roadmap inteiro (skill github-publish).

日本語の概要は準備中です。原文の説明を表示しています。

Estrutura narrativa para copy — PAS, BAB, AIDA, PASTOR, Jornada do Herói e Golden Circle. Use quando o usuário disser "storytelling", "contar a história", "narrativa da marca", "o texto está sem fio condutor", "case de sucesso", ou quando a página precisar de narrativa em vez de argumento listado.

日本語の概要は準備中です。原文の説明を表示しています。

Escreve roteiros de vídeo e VSL — gancho, abertura, desenvolvimento, prova, CTA e fechamento, com marcação de tempo e indicação visual. Use quando o usuário disser "roteiro", "VSL", "script de vídeo", "vídeo de vendas", "reels", "roteiro para YouTube".

日本語の概要は準備中です。原文の説明を表示しています。

Make a GTK4/libadwaita app accessible — accessible labels and roles, tooltips on icon-only buttons, keyboard navigation, contrast, and testing with Orca/Accerciser. Use when fixing a11y issues or running the accessibility category of a HIG audit.

日本語の概要は準備中です。原文の説明を表示しています。

武將數值平衡調校 SKILL — 以雙軸演算法（maxStat + avg5）自動計算 rarityTier 與 characterCategory，並執行 EP 重算、適性校驗、因子分派、稀有度分布統計。USE FOR: 新武將入庫前的數值校準、全量武將的平衡盤點、稀有度自動分類。DO NOT USE FOR: 故事生成（用 general-story-writer）、資料爬取匯入（用 general-data-pipeline）。

日本語の概要は準備中です。原文の説明を表示しています。

武將故事批次生成 SKILL — 根據武將的 GeneralConfig（含 characterCategory + rarityTier），生成 historicalAnecdote、bloodlineRumor、六格 storyStripCells 故事條。USE FOR: 填充 generals.json 中的 "???" 欄位、批次生成武將背景故事、統一故事風格。DO NOT USE FOR: 數值平衡調整（用 general-balance-tuner）、資料爬取（用 general-data-pipeline）。

日本語の概要は準備中です。原文の説明を表示しています。

Assembles a genome de novo from Illumina short reads with SPAdes (isolate/careful/sc/meta/plasmid/rna modes), MEGAHIT (low-memory, huge datasets), Unicycler (bacterial finishing/hybrid), MaSuRCA (large hybrid), ABySS (Bloom-filter), and Platanus (heterozygous diploids), using multi-k de Bruijn graphs. Covers the repeat-resolution limit, why N50 plateaus at the genome not the depth, GenomeScope2 k-mer profiling first, the heterozygosity/haplotig trap, error-correction erasing rare alleles, GC dropout, and NG50/auN/BUSCO reporting. Use when assembling a bacterial isolate, fungal, small-eukaryotic, single-cell, or metagenome genome from Illumina reads, or when deciding whether short reads can even produce the assembly being asked for.

日本語の概要は準備中です。原文の説明を表示しています。

Detects and removes doublets/aggregates from flow, spectral, and mass cytometry before clustering or quantification. Covers FSC-A vs FSC-H singlet discrimination (the Area-Height non-proportionality, not a 1D area gate), FSC-W/SSC width gating, CyTOF Gaussian discrimination parameters (Center/Offset/Width/Residual/Event_length) and DNA intercalator gating, and the residual heterotypic conjugates that survive scatter gating and masquerade as double-positive populations. Use when filtering aggregates before phenotyping, choosing a doublet method for flow vs CyTOF, or diagnosing a suspicious double-positive cluster.

日本語の概要は準備中です。原文の説明を表示しています。

Analyzes base-editing screens for variant function. Covers library design (Sanson 2020 GRACE, Hanna 2021 BRCA1/2 SNV scanning, Cuella-Martin 2021), CBE vs ABE chemistry choice (BE3/BE4 vs ABE7.10/ABE8.20/ABE8e), editing-window math (positions 4-8 from PAM-distal end, wider for ABE8e), bystander-edit quantification and the variant-call ambiguity it creates, sgRNA-efficiency filtering before hit calling, indel byproduct interpretation, the substitution-vs-indel diagnostic, variant annotation against ClinVar / COSMIC, and the Broad be-validation-pipeline. Use when designing a BE variant screen, choosing CBE vs ABE for a specific edit, interpreting bystander-confounded hits, distinguishing functional signal from indel artifact, integrating CRISPResso2 output with screen scoring, or deciding BE vs PE for SNV installation.

日本語の概要は準備中です。原文の説明を表示しています。

Build interactive HTML/web visualizations with plotly (Python/R), bokeh (Python), and gganimate/plotly frames for animation, with awareness of current Kaleido static-export model (post-orca-EOL), HTML file-size bloat, and the limits of interactive-only output for journal submission. Use when producing zoomable/hoverable plots for notebook EDA, supplementary HTML, dashboards, or animated time-course / iteration visualizations.

日本語の概要は準備中です。原文の説明を表示しています。

Annotate CLIP-seq peaks or crosslink sites to RNA features (5'UTR, CDS, 3'UTR, intron, splice junction, snoRNA, tRNA, ncRNA, repeat elements) with ChIPseeker, RCAS, RBP-Maps (Yeo splicing regulatory maps), and bedtools, applying feature-priority hierarchies, transcript-context resolution, and metagene aggregation. Use when characterizing where in transcripts an RBP binds, comparing peak distribution across regions, generating splicing-regulatory maps relative to alternative-splicing events, or distinguishing exonic vs intronic vs UTR binding.

日本語の概要は準備中です。原文の説明を表示しています。

Quantify homologous recombination deficiency (HRD) from tumor copy number using the three genomic-scar metrics — loss of heterozygosity (LOH), large-scale state transitions (LST), and telomeric allelic imbalance (TAI) — with scarHRD, and via the whole-genome HRDetect and CHORD models. Covers the genomic instability score, the PARP-inhibitor clinical context, whole-genome-doubling correction, and the scar-versus-state distinction. Use when computing an HRD score for PARP-inhibitor eligibility, deriving LOH/LST/TAI scars from allele-specific copy number, deciding between scar-based and mutational-signature HRD methods, or interpreting an HRD result in a BRCA-reverted or low-purity tumor.

日本語の概要は準備中です。原文の説明を表示しています。

Calls HLA class I and class II alleles at 2/4/6/8-field resolution from WGS/WES/RNA-seq/long-read data using OptiType, HLA-LA, T1K, Polysolver, HLA-HD, arcasHLA, StarPhase, or HIBAG imputation. Use when typing for HSCT, solid-organ transplant, neoantigen prediction, PGx screening (B*57:01, B*15:02, etc.), or disease-association studies, with reconciliation across tools and IPD-IMGT/HLA version mismatch handling.

日本語の概要は準備中です。原文の説明を表示しています。

Extracts and assigns COSMIC v3.4 mutational signatures (84 SBS / 11 DBS / 18 ID / 24 CN / 16 SV) from somatic VCFs using SigProfilerSuite, MutationalPatterns, MuSiCal mvNMF, SigNet, or HRDetect. Use when characterizing DNA-damage etiology (BRCA1/2 HRD, MMR-D, POLE, APOBEC3A, UV, tobacco, aflatoxin, 5-FU/SBS17b, platinum, colibactin SBS88), routing PARP inhibitor decisions, or auditing de novo extraction vs refit choice for cohort size.

日本語の概要は準備中です。原文の説明を表示しています。

Performs molecular similarity searching using Tanimoto, Tversky, Dice, and cosine coefficients on bit/count fingerprints with explicit choice rules for symmetric vs asymmetric measures, scaffold-hopping vs lead-optimization regimes, activity-cliff diagnosis, and large-library nearest-neighbor methods (BulkTanimoto, Annoy MHFP6, USRCAT). Use when ranking compounds by structural resemblance to a query, clustering libraries, finding analogs, or diagnosing activity cliffs.

日本語の概要は準備中です。原文の説明を表示しています。

Performs 3D shape-based similarity searching using ROCS (OpenEye), USRCAT (ultra-fast), Open3DAlign (RDKit), ESPSim (electrostatic), and ShaEP with explicit handling of Tanimoto-Combo (shape + color), shape vs ECFP4 complementarity, conformer-ensemble searching, alignment optimization, and scaffold hopping. Use when searching for shape-mimicking compounds with different scaffolds, identifying bioisosteric replacements, prospective scaffold hopping, or expanding hit series beyond 2D similarity.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks about driver fatigue management — circadian rhythm impact on driving, recognizing fatigue, fatigue risk management programs (FRMS), night driving safety, microsleep recognition, drowsiness detection technology, and how HOS rules relate to actual fatigue (and why 11-hour driving is risky even when legal). Reference FMCSA Fatigue Management research.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill to create CodeTour .tour files — persona-targeted, step-by-step walkthroughs that link to real files and line numbers. Trigger for: "create a tour", "make a code tour", "generate a tour", "onboarding tour", "tour for this PR", "tour for this bug", "RCA tour", "architecture tour", "explain how X works", "vibe check", "PR review tour", "contributor guide", "help someone ramp up", or any request for a structured walkthrough through code. Supports 20 developer personas (new joiner, bug fixer, architect, PR reviewer, vibecoder, security reviewer, and more), all CodeTour step types (file/line, selection, pattern, uri, commands, view), and tour-level fields (ref, isPrimary, nextTour). Works with any repository in any language.

日本語の概要は準備中です。原文の説明を表示しています。

Pixel art w/ era palettes (NES, Game Boy, PICO-8).

日本語の概要は準備中です。原文の説明を表示しています。

Creates comprehensive post-incident documents with timeline, root cause analysis, contributing factors, action items, and ownership. Follows SRE best practices for blameless postmortems. Use for "postmortem", "incident review", "RCA", or "post-incident".

日本語の概要は準備中です。原文の説明を表示しています。

Climb the browser ladder — start free, escalate only when needed. L1 (fetch) → L2 (local Playwright) → L3 (BrowserCat) → L4 (Browserless.io for CAPTCHA/bot bypass).

日本語の概要は準備中です。原文の説明を表示しています。

Promote Doppel world builds across social platforms. Use when the agent wants to share builds on Twitter/X, Farcaster, Telegram, or Moltbook to drive observers, grow reputation, and recruit collaborators.

日本語の概要は準備中です。原文の説明を表示しています。

Handles comprehensive resource acquisition, categorization, and asset management. Adaptive to various sources (URLs, PDFs, local paths) and centralizes media in a lowercase 'assets' folder.

日本語の概要は準備中です。原文の説明を表示しています。