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概要と使いどころ
Tests in real browsers. Use when building or debugging anything that runs in a browser. Use when you need to inspect the DOM, capture console errors, analyze network requests, profile performance, or verify visual output with real runtime data via Chrome DevTools MCP.
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Select and implement the right Azure compute platform for a workload. Decision tree guides choice based on workload characteristics, scaling needs, and operational maturity. Covers serverless, PaaS, containers, Kubernetes, and IaaS patterns.
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Verify and test Jupyter notebooks with iterative fixing. Arguments: [target] [--quick] [--fix] [--python-only] [--dotnet-only]
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Use when building Grafana dashboards backed by Prometheus, Loki, or Tempo, designing PromQL/LogQL queries, wiring template variables, setting alert rules, building SLO dashboards, or maintaining dashboards as code. Triggers: rate() vs increase() confusion, irate vs rate, label_replace, recording rules, alerting rule expressions, multi-dimensional template variables, ad-hoc filters, dashboard JSON model, provisioning via Terraform/grafonnet, p99 / histogram_quantile usage. NOT for Datadog/New Relic dashboards (vendor-specific), Grafana plugin development, or Loki ingestion pipeline tuning.
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Use when CPU usage is high, memory grows unboundedly, goroutines leak, mutex contention shows in traces, or escape analysis suggests excess heap allocation. Triggers: net/http/pprof endpoint exposure, go tool pprof analysis, flamegraph generation, allocs vs inuse_space heap profiles, runtime/trace event timeline, mutex profiling, block profiling, goroutine dumps, GC pressure measurement. NOT for non-Go languages, distributed tracing (use OpenTelemetry skill), or production telemetry pipelines.
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Write production-grade Dockerfiles, docker-compose configurations, multi-stage builds, and container optimization. Activate on "Dockerfile", "docker", "docker-compose", "container", "multi-stage build", "docker image", "container optimization", "docker security". NOT for Kubernetes orchestration, cloud-specific container services (ECS, Cloud Run), or CI/CD pipelines (use github-actions-pipeline-builder).
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Use when optimizing Docker builds with BuildKit, designing multi-stage Dockerfiles, leveraging cache mounts, building multi-arch images, choosing distroless vs alpine, or signing images with cosign. Triggers: docker build slow, layer cache not hitting, npm install / pip install reruns, multi-stage final image bloat, glibc vs musl issues, RUN --mount=type=cache, BUILDKIT_INLINE_CACHE, registry cache exporters, qemu emulation for arm64, COPY ordering for cache. NOT for Docker daemon admin, Kubernetes-specific image policies, container security scanning workflows, or Buildpacks (different paradigm).
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Multi-stage Docker build optimizer for minimal, secure production images. Activate on: Dockerfile optimization, multi-stage build, distroless image, container size reduction, Docker security scanning, BuildKit features. NOT for: container orchestration (use kubernetes-manifest-generator), CI/CD pipelines (use github-actions-pipeline-builder), runtime container config (use environment-config-manager).
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Analyze hierarchical DAG-like systems by separating undirected substrate from ordering metadata, then classifying diamonds, mixers, shortcuts, and hidden cycle structure. Use for workflow architecture, citation or genealogy DAG comparison, and information-flow diagnosis. NOT for ordinary cycle detection in arbitrary graphs, one-node runtime debugging, or systems with no real ordering constraint.
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Tracks cumulative LLM costs across DAG execution and makes real-time decisions to stay within budget. Downgrades models, skips optional nodes, or stops early when cost exceeds thresholds. Use when managing execution budgets, analyzing cost breakdowns, or optimizing model routing for cost. Activate on "cost budget", "too expensive", "reduce cost", "cost optimization", "model downgrade", "budget exceeded". NOT for LLM model selection logic (use llm-router), pricing comparisons across providers, or billing/invoicing.
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Blue-green and canary deployment orchestrator with traffic shifting and automated rollback. Activate on: blue-green deployment, canary release, rolling deployment, traffic shifting, rollback automation, progressive delivery, Argo Rollouts, Flagger. NOT for: K8s manifest generation (use kubernetes-manifest-generator), CI/CD pipeline setup (use github-actions-pipeline-builder), monitoring (use monitoring-stack-deployer).
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Strategic patterns for solving intractable problems through cascading approximation, self-improvement, and heterogeneous evaluation from DeepMind's AlphaGo system
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Spin up a coordinated multi-agent swarm (hierarchical or mesh topology) to work a complex, multi-file task on a ruOS desktop. Use when a task needs 3+ agents working in parallel with anti-drift coordination; skip for single-file edits.
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Persistent, adaptive vector memory for agents on a ruOS desktop — a Rust-native vector database with local semantic embeddings, HNSW retrieval, and graph relationships. Use to remember and recall across sessions with no database server or API key.
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Self-learning pre/post-task hooks and model/task routing from the Ruflo CLI, on a ruOS desktop. Use to add coordination, formatting, and learning around Claude Code operations, or to route work by complexity/cost.
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Store and semantically search agent memory with ONNX vector embeddings on a ruOS desktop, using the Ruflo CLI. Use to persist patterns/decisions across sessions and recall them by meaning rather than exact key.
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Auto-activate for Dockerfile, compose, Railway, Cloud Run, GKE, systemd, Kubernetes, Terraform, deploy scripts, or granian/litestar run at runtime. Not for packaging artifacts.
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Assesses the quality and completeness of a genome annotation with BUSCO (conserved single-copy ortholog recovery), OMArk (proteome completeness, consistency, and contamination), CheckM2 (prokaryotic completeness/contamination), and a gene-set sanity panel (gene count, mono-exonic fraction, protein-length distribution, mRNA:gene ratio, coding density). Covers the assembly-BUSCO-vs-proteome-BUSCO diagnostic, what BUSCO-Duplicated really means, why gene count is a vanity metric, and the QC of transferred annotations. Use when judging whether an annotation is good enough to publish or submit, diagnosing a suspect annotation, or comparing annotation completeness across pipelines.
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Assigns pathogen lineages (SARS-CoV-2 Pangolin UShER mode; Nextclade clade + QC; pango-designation alias resolution) and tracks variant frequencies over time using Nextstrain (Augur + Auspice), wastewater deconvolution (Freyja, COJAC, alcov, lineagespot), lineage-fitness modelling (multinomial logistic), and recombinant detection (3SEQ, RDP4, Bolotie). Covers Pangolin pangolin-data and Nextclade dataset version pinning (mandatory; lineage-defining mutations change with dataset), Freyja barcode forward-only date constraint, ARTIC primer scheme churn (V3/V4/V4.1/V5.3.2/Midnight) with dropout regions, and recombinant X-prefix designation lag. Use when assigning Pango lineages and Nextclade clades to viral consensus sequences, building Nextstrain Augur surveillance pipelines, deconvolving wastewater into lineage frequencies with Freyja, tracking lineage frequencies over time, handling ARTIC primer dropouts, or running surveillance for SARS-CoV-2/influenza/Mpox/RSV/H5N1/measles.
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Visualises RNA-modification data with transcript-feature metagene plots (Guitar GuitarPlot; MetaPlotR; deepTools computeMatrix scale-regions), peak-centred heatmaps (ComplexHeatmap; deepTools plotHeatmap), IP-vs-input paired browser tracks (log2 IP/input bigWig via deepTools bamCompare; pyGenomeTracks; Gviz; IGV/UCSC track hubs), DRACH sequence-logo plots (ggseqlogo; MEME), feature-distribution stacked bars, and volcano/MA plots for differential modification. Establishes stop-codon enrichment in the metagene plot as the biological QC anchor for any MeRIP dataset (Dominissini 2012; Meyer 2012). Use when producing the canonical metagene plot with stop-codon enrichment as a QC anchor, building paired IP/input genome-browser tracks at single-locus resolution, plotting peak-centred heatmaps clustered by condition, summarising peak distribution across transcript features, generating DRACH motif logos as sanity checks, rendering volcano plots of differential m6A, or reproducing the stop-codon enrichment plot.
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Designs genomics experiments so technical nuisance variation (batch, lane, plate, flow cell, operator, reagent lot, processing day) is balanced against the biological variable of interest and therefore estimable rather than confounded, using constrained sample-to-batch assignment (designit, OSAT), the confounder/mediator/collider distinction, and the principle that no post-hoc correction recovers a fully confounded design. Covers detecting hidden batches with surrogate variable analysis, a decision table for downstream correction (ComBat-seq, RUVSeq, SVA) whose execution is deferred to differential-expression/batch-correction, and reproducibility metadata. Use when assigning samples to sequencing batches/lanes/plates, avoiding batch-condition confounding, deciding whether a design is salvageable by correction, choosing a correction method, or estimating the number of hidden batches. For the experimental unit, randomization, and blocking concepts see experimental-design/randomization-blocking.
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Controls error rates across thousands of simultaneous tests in genomics discovery using false-discovery-rate methods (Benjamini-Hochberg 1995; Benjamini-Yekutieli 2001 for arbitrary dependence; Storey q-value with pi0 estimation; local FDR; independent filtering Bourgon 2010; covariate-weighted FDR via IHW Ignatiadis 2016), plus family-wise error control (Bonferroni, Holm) and the GWAS genome-wide threshold. Covers the FDR-versus-FWER choice as the discovery-versus-confirmatory distinction, the dependence assumptions behind BH (PRDS) versus BY, pi0 estimation, the independent-filtering and false-coverage-rate traps, and reproducibility ranking via IDR (Li 2011). Use when correcting p-values from genome-wide tests, choosing between BH/BY/q-value/Bonferroni, setting an FDR threshold, applying IHW or independent filtering, or interpreting q-values. For confirmatory trials with few pre-specified endpoints (closed testing, graphical/gatekeeping), see clinical-biostatistics/multiplicity-graphical.
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Calculates statistical power for high-dimensional genomics experiments (bulk RNA-seq, scRNA-seq, ATAC-seq, ChIP-seq, methylation, proteomics) under negative-binomial count models using RNASeqPower, PROPER, and simulation via powsimR, distinguishing per-gene from marginal (transcriptome-wide) power, the role of mean expression and dispersion, and the sequencing-depth-versus-replicate tradeoff. Covers simulation as the honest default for overdispersed counts, FDR-aware average power versus single-test power, observed/post-hoc power as an anti-pattern, and the winner's-curse / Type-S / Type-M consequences of underpowering. Use when planning replicate number for a sequencing experiment, deciding whether to add depth or samples, choosing closed-form versus simulation power, estimating power from pilot dispersions, or justifying replication in a grant. For clinical-trial power see clinical-biostatistics/power-and-sample-size; for the inverse sample-size question see experimental-design/sample-size.
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Structures biological experiments so inference is valid by construction, covering Fisher's principles (randomization, replication, local control), the experimental-vs-observational unit distinction and pseudoreplication (Hurlbert 1984; Lazic 2018), randomization mechanics (complete, restricted, stratified, rerandomization, run-order), blocking layouts (randomized complete block, Latin square, incomplete block), factorial designs and interactions, and the split-plot/nested error strata hidden inside multi-batch genomics. Use when deciding the experimental unit and what counts as a replicate, planning randomization and run order, choosing a blocked/factorial/split-plot/nested layout, avoiding pseudoreplication in cell-culture or animal studies, or specifying the random-effects structure of the analysis model. For assigning samples to sequencing batches/lanes/plates and batch-effect correction see experimental-design/batch-design; for regulated clinical-trial randomization see clinical-biostatistics.
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