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Performs multiple sequence alignment of proteins with EBI Clustal Omega. Use when you need to align multiple sequences to assess similarity, domain conservation, or key residue conservation. Supports up to 4000 sequences and a maximum file size of 4 MB. Do not use to search for homologous proteins in a database (use MMseqs2, BLAST), align non-protein sequences (DNA, RNA), perform structural alignment (use Foldseek, PyMOL), or if you only have a single sequence.

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Transforms a rough idea into a detailed design document with implementation plan. Follows Prompt-Driven Development — iterative requirements clarification, research, design, and planning.

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Browser automation CLI for AI agents. Use when the user needs to interact with websites, including navigating pages, filling forms, clicking buttons, taking screenshots, extracting data, testing web apps, or automating any browser task. Triggers include requests to "open a website", "fill out a form", "click a button", "take a screenshot", "scrape data from a page", "test this web app", "login to a site", "automate browser actions", or any task requiring programmatic web interaction.

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Converts VitePress/GFM wiki markdown to Azure DevOps Wiki-compatible format. Generates a Node.js build script that transforms Mermaid syntax, strips front matter, fixes links, and outputs ADO-compatible copies to dist/ado-wiki/.

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Extract text, tables, and structured data from documents using Azure Document Intelligence (@azure-rest/ai-document-intelligence). Use when processing invoices, receipts, IDs, forms, or building custom document models.

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Sysinternals DebugView CLI (DbgViewCli) for capturing and analyzing usermode and kernel-mode Windows debug output from the command line. USE FOR: capturing OutputDebugString output, kernel DbgPrint/KdPrint capture, boot-time debug logging, remote debug monitoring, filtering debug output by PID or process name, crash dump analysis, automated debug capture with bounded execution. DO NOT USE FOR: non-Windows platforms, application-level logging frameworks (log4j, serilog), Azure Monitor or cloud telemetry, ETW tracing (use WPR/xperf instead), user-mode crash dumps (use WinDbg). Triggers: "debug output", "DbgView", "DebugView", "kernel debug", "capture debug logs", "boot logging", "OutputDebugString", "DbgPrint", "KdPrint", "remote debug monitor", "debug capture CLI".

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Real-time depth map privacy transforms using Depth Anything v2 (CoreML + PyTorch)

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WikiPathways enrichment using clusterProfiler and rWikiPathways. Use when analyzing gene lists against community-curated open-source pathways. Performs over-representation analysis and GSEA for 30+ species.

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Reactome pathway enrichment using ReactomePA package. Use when analyzing gene lists against Reactome's curated peer-reviewed pathway database. Performs over-representation analysis and GSEA with visualization and pathway hierarchy exploration.

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Analyzes isoform switching events and functional consequences using IsoformSwitchAnalyzeR. Predicts protein domain changes, NMD sensitivity, ORF alterations, and coding potential shifts between conditions. Use when investigating how splicing changes affect protein function.

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Analyzes cfDNA methylation patterns for cancer detection using cfMeDIP-seq or bisulfite sequencing with MethylDackel. Identifies cancer-specific methylation signatures and performs tissue-of-origin deconvolution. Use when using methylation biomarkers for early cancer detection or minimal residual disease.

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Spillover compensation and data transformation for flow cytometry. Covers compensation matrix calculation, application, and biexponential/arcsinh transforms. Use when correcting spectral overlap between fluorophores or transforming data for analysis.

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Guidance for SPR and BLI binding characterization experiments. Use when: (1) Planning binding kinetics experiments, (2) Troubleshooting poor/no binding signal, (3) Interpreting kinetic data artifacts, (4) Choosing between SPR vs BLI platforms.

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Browse the web for any task — research topics, read articles, interact with web apps, fill forms, take screenshots, extract data, and test web pages. Use whenever a browser would be useful, not just when the user explicitly asks.

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Assess and plan migrations from existing VPN, SWG, or SASE platforms to Cloudflare One, including policy mapping, parity gaps, and rollout.

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Use when the user asks to "track our share of voice", "what share of the conversation do we own vs competitors", or "trend our SOV this quarter"; computes SOV% = brand mentions ÷ (brand + competitor panel mentions) per platform per period on a LOCKED competitor panel — a panel switch invalidates the trend (restart the series and log the break; the ECHO O3 rule) — plus a sentiment-weighted SOV variant (sentiment labeled Estimated unless human-coded) and a Wikipedia-pageviews attention-share alternative; built from keyless listening connectors, gdelt.py news echo, and user exports — public counts only, closed platforms are never scraped. Not for backlink or offsite SEO signals — use offsite-signal-analyzer. 声量份额/竞品声量对比/提及份额/注意力份额

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Use when the user asks to "build the norm card for this platform", "what are the char limits and visible-fold cutoffs here", "is the LinkedIn link-in-first-comment thing documented or folklore", or "which of our platform cards are stale"; maintains the dated, versioned per-platform norm cards in the references/platforms/ pack — char limits, visible-fold cutoffs, hashtag norms, format/aspect specs, link and first-comment placement, disclosure-label mechanics, algorithm emphases (e.g. 小红书 search+saves weighting) — every row labeled platform-documented (official doc, Measured) or Estimated-folklore (named source) with last-verified and review-by dates, and any card past its review date flagged stale rather than trusted. Extends the single pack in place; never forks a second one. Not for picking which channels to run — use channel-portfolio-planner. 平台规范卡/字符限制/折叠线/话题标签/算法侧重/过期标记

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Use when the user asks to "build audience segments from my customer list", "make value-based / lookalike seed lists", "set up exclusion / suppression segments", or "map audiences to funnel stages across platforms"; turns the user's OWN customer/CRM/GA4 export into seed audiences, value-based lookalike SEED lists, exclusion/suppression segments, and a cross-platform funnel-stage targeting map, informing the ROAS A (Audience) dimension. Not for building account structure or match types — use campaign-architect; not for organic SERP intent — use keyword-research. 付费广告受众分群/种子人群/排除人群/相似人群种子

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Expert guide for Gradle 9 builds in apache/grails-core on the 8.0.x line - multi-project topology, convention plugins, BOM platforms, dependency rules, task configuration hygiene, and repo-specific patterns that override generic Gradle docs

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Apply the Law of Closure — the eye completes implied shapes from partial forms. Use when reducing visual weight by dropping borders or letting negative space suggest structure. For explicit containers, use `law-of-common-region`.

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Performs code upgrades, migrations, and transformations using the AWS Transform (ATX) CLI. Use when upgrading language versions, migrating AWS SDKs, migrating frameworks (Angular, Vue.js, Spring Boot, React), upgrading libraries, optimizing performance, migrating x86 to Graviton, analyzing codebases / generating documentation, or defining custom transformations with natural language. Runs locally on a few repositories or at scale across hundreds via AWS Batch/Fargate.

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Builds, runs, debugs, and operates event-driven applications using EventBridge Event Bus - a managed, centrally governed publish/subscribe event bus that an organization can share across many teams and accounts. Applicable when workloads need event-driven architectures, decoupling, choreography, asynchronous integration, pub/sub, fan-out, event ordering, delivered in sequence, event router, event broker, event bus, event store, event retention, event replay, deduplication, avro, protobuf, cloudevents, uses events or messages. Designed as a centrally managed event bus platform allowing governance and control - such as subscriber control, revocation, per-account throttling - while giving application owners flexibility across an organization spanning multiple accounts, with end-to-end open-standards observability and cost allocation. Also helps reduce costs in high fan-out scenarios where multi-account forwarding adds cost. A serverless, managed alternative to self-managed event streaming platforms.

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Migrates vibe-coded web applications to AWS. Handles the full workflow from analysis through migration to deployment, producing deployable AWS Blocks infrastructure code. Supports full-stack apps built with vibe-coding platforms (Lovable, Bolt.new, Replit) and frontend web applications and websites: React, Vue, Angular, Next.js, Nuxt, Astro, SvelteKit, Gatsby, Vite, Svelte, Solid, Docusaurus, and others (static sites, SPAs, and SSR frameworks with static export). Triggers on: launch with AWS, launch on AWS, deploy to AWS, migrate to AWS, host my app on AWS, move my app to AWS, transfer my app to AWS. Activates when the user wants to migrate a vibe-coded app or frontend web app to AWS, even if they don't say 'migrate' explicitly.

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Guides an end-to-end data-warehouse migration to Amazon Redshift — discovery, schema/SQL/stored-procedure/macro/script conversion, data migration, validation, performance comparison, and reporting. Source-routed via `references/<source>/`; Teradata (Vantage) is the supported source; additional sources are added as their own `references/<source>/` sets. Text-only knowledge (no executable code) — the AI generates all execution at runtime. Applies when a user wants to migrate Teradata to Amazon Redshift, convert Teradata DDL/SQL/stored procedures/macros/BTEQ to Redshift/RSQL, or assess Teradata-to-Redshift migration complexity. Applies only to migrations targeting Amazon Redshift; migrations to other platforms (Snowflake, BigQuery, Databricks, etc.) are out of scope regardless of source. Does not cover general Redshift administration, performance tuning, or troubleshooting of existing Redshift clusters (no migration involved), or sources not listed under references/.

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