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Retrieve protein and nucleotide sequences from NCBI databases using E-utilities. Supports direct accession lookup, CDS translation, gene+organism search, locus lookup, PubMed-linked sequences, patent protein extraction, and organism+length fallback search. Use when you need to fetch biological sequences by accession, gene name, locus tag, PubMed ID, or patent number.

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Filter reads by quality scores, length, and N content using Trimmomatic and fastp. Apply sliding window trimming, remove low-quality bases from read ends, and discard reads below thresholds. Use when reads have poor quality tails or require minimum quality for downstream analysis.

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Analyze PacBio Iso-Seq data for full-length isoform discovery and quantification. Use when characterizing transcript diversity or identifying novel splice variants.

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Quality control for long-read sequencing data using NanoPlot, NanoStat, and chopper. Generate QC reports, filter reads by length and quality, and visualize read characteristics. Use when assessing ONT or PacBio run quality or filtering reads before assembly or alignment.

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Filter and select sequences by criteria (length, ID, GC content, patterns) using Biopython. Use when subsetting sequences, removing unwanted records, or selecting by specific criteria.

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Preprocesses cell-free DNA sequencing data including adapter trimming, alignment optimized for short fragments, and UMI-aware duplicate removal using fgbio. Applies cfDNA-specific quality thresholds and fragment length filtering. Use when processing plasma cfDNA sequencing data before downstream analysis.

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Analyze historical downtrend durations and generate interactive HTML histograms showing typical correction lengths by sector and market cap.

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Use when the user asks to "generate subject line variants", "pre-score my subject lines", or "will this subject get truncated / trigger spam filters"; produces a labeled subject + preheader variant set and a per-variant heuristic pre-score card — spam-trigger flags, length/truncation across desktop + mobile, emoji-count, and the inbox preview render (from-name + subject + preheader) — before any test is run. Not for the body copy or CTA — use email-creative-builder; not for the A/B test design or significance read — use send-experiment-designer; not for the profile-weighted EQS or the S1/S2/N1/D1 vetoes — use email-quality-auditor. 邮件主题行生成/主题行预打分/截断与垃圾词检查

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基于国自然标书篇幅预算标准；检查目标标书篇幅并总结差距；给出针对性优化建议；在尽量不改变原意的前提下扩写/压缩到达标。

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Create a modular type scale with size, weight, and line-height relationships. Use when establishing typographic structure. For line length only use `readable-measure`; for judging type on an existing screen use `critique-typography` (visual-critique).

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Set line length and measure for comfortable reading across type sizes and breakpoints. Use when tuning body text. Covers measure only — for the full size and weight scale, use `typography-scale`.

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HTTP request smuggling and desynchronization testing. Use when front proxies, CDNs, or load balancers disagree with the origin on message framing (Content-Length vs Transfer-Encoding), on HTTP/2→HTTP/1 translation, or when exploring client-side desync via browser fetch pipelines.

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Hash attack playbook. Use when exploiting length extension, MD5/SHA1 collisions, HMAC timing leaks, birthday attacks, or hash-based proof of work in CTF and authorized testing scenarios.

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Create and manage writing personas with NNGroup 4-dimension tone framework (Funny-Serious, Formal-Casual, Respectful-Irreverent, Enthusiastic-Matter-of-fact). Personas define readability targets, sentence length distribution, vocabulary tier, contraction frequency, and summary box label. Used by blog-write and blog-rewrite to enforce consistent voice. Use when user says "persona", "voice", "tone", "writing style", "brand voice", "create persona", "use persona".

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Post-writing SEO validation with pass/fail checklist covering title tag length and keyword placement, meta description quality, heading hierarchy and keyword density, internal/external link audit with anchor text analysis, canonical URL verification, Open Graph meta tags (og:title, og:description, og:image), Twitter Card validation, structured data presence and validity, URL structure optimization, and image alt text presence. Produces prioritized fix list with specific recommendations. Use when user says "seo check", "check seo", "validate seo", "blog seo", "seo validation", "on-page seo", "title tag check", "meta description check", "heading check", "link audit".

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Audit and score blog posts on a 5-category 100-point scoring system covering content quality, SEO optimization, E-E-A-T signals, technical elements, and AI citation readiness. Includes advisory editorial style diagnostics (sentence-length variation, configured phrase lists, vocabulary sampling) that never infer authorship or affect scoring. Supports export formats (markdown, JSON, table) and batch analysis with sorting. Generates prioritized recommendations (Critical/High/Medium/Low) with specific fixes. Works with any format (MDX, markdown, HTML, URL). Use when user says "analyze blog", "audit blog", "blog score", "check blog quality", "blog review", "rate this blog", "blog health check".

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Analyze tau2-bench task export files to identify exactly where and why tasks failed. Map failures to specific conversation rounds, show root causes, check whether relevant documentation was available, and cite sources. Use this skill whenever you need to debug a tau2-bench evaluation, understand why an agent made wrong decisions, identify missing knowledge, or review task performance—especially when context length, eligibility criteria, or policy compliance might be at fault.

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Transform lengthy academic papers into concise, structured 250-word abstracts.

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Reference for the EBCDIC "overpunch" / zoned-decimal sign convention where the units position of a numeric field is replaced with a letter that encodes both a digit and a sign. Useful when reading mainframe-style fixed-length tapes whose amount fields appear as digits followed by a letter (e.g. "0000000000123D" or "0000000000045M").

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Case studies of television series writing (剧集案例库) from primary texts; Sorkin's six West Wing teleplays (act-page tables, eight act-out types); Chase's five Sopranos scripts (the 35-beat A/B/C/D recipe, what a pilot refuses to explain); Fellowes's annotated Downton Abbey season two (19 storylines, 419 footnotes); Waller-Bridge's Fleabag Scriptures (the six-episode half-hour template); Calvisi's eight minute-by-minute pilot beat sheets (Breaking Bad, Mad Men, Game of Thrones and more); Landau's 47-series structure appendix; Miller's Hannibal and HTGAWM breakdowns; Goldberg & Rabkin's beat sheets; Sakamoto Yuji's We Made a Beautiful Bouquet (a feature) and Noh Hee-kyung's The Most Beautiful Goodbye. Use when you want a worked example of how an episode or pilot is built, need real act lengths, scene counts or storyline grids to imitate, are breaking a multi-thread ensemble episode, designing a bottle episode or a finale, writing a relationship or family drama, or ask 某一集是怎么写的 / 这部剧的结构表.

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Half-hour television comedy craft (半小时情景喜剧/笑点力学/困境机器/多机位单机位格式) — merged from Evan S. Smith's Writing Television Sitcoms (premise-driven comedy in three levels, nine predicaments, six character mixes, setup/punchline mechanics, toppers, running gags, ten comedic nuances, format rules), Neil Landau's sitcom seven-step formula and comedy-room models, Richard Blum's act-length and set-count parameters, Phoebe Waller-Bridge's Fleabag scripts (direct address as a season-long device, six-episode skeleton), Pamela Douglas on dramedy, Kam Miller on complementary comic traits, and Jesse Armstrong on jokes carrying tragedy. Use when writing or fixing a sitcom episode or spec, raising joke density per page, designing a comic predicament that generates stories, choosing between multi-camera and single-camera format and page counts, structuring cold open/acts/tag, writing a half-hour pilot, running a comedy punch-up, or making a drama funny without shifting genre.

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Worked full-length scripts of banqiang-system Chinese opera with their creation records (板腔体全本语料库). Primary texts; 翁偶虹《锁麟囊》 (the revised score edition, 65 arias, 15 self-account-vs-score checks), 汪曾祺等《沙家浜》 (the 1965 text, not the 1970 one; 36 arias, a 12-row two-version diff), 田汉《白蛇传》 (the 1955 book, not the web revision; 16 scenes, 王瑶卿说腔五条), 杨兰春《朝阳沟》 (1978 revival, 50 arias, 口语入唱, per-scene sung/spoken ratios), 魏明伦《潘金莲》 (跨时空, missing pages), 罗怀臻's 越剧《李清照》《真假驸马》, 淮剧《金龙与蜉蝣》, 甬剧《典妻》; plus index tables of 翁偶虹's 60 创作记 and 范钧宏's seven 改编札记. Use when you want to see how a 唱工戏 lays out its 板式, need real scene counts, aria counts and 辙口 to imitate, want the road from raw material to 审腔, are writing a 现代戏 or colloquial lyrics, or compare one author across two声腔 systems. Method is in sw-chinese-opera-banqiang; this skill holds the worked scripts and their creation records.

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Episode and season structure for TV series (剧集单集与季的结构) — teasers and cold opens, broadcast four/five/six-act grids with page anchors, three tests for finding the invisible acts in streaming scripts, act-out and cliffhanger taxonomies, Oberg's four information tools, A/B/C/runner weaving and scene-count ratios, the Calvisi pilot beat sheet, five-part scene structure, and season shape (tentpoles, bottle and container episodes, two-parters, finales). Merged from Calvisi, Douglas, Oberg, Landau, Goldberg & Rabkin, Miller, Rabkin and Blum, plus act lengths measured from published West Wing, Succession, Sopranos, Downton and Fleabag scripts. Use when breaking an episode into acts, deciding how many acts a script needs, placing act outs and cliffhangers, designing a teaser, weaving A/B/C stories and runners, beating out a one-hour pilot, auditing an episode that has no shape, reverse-engineering a show's structure, or planning a season's episode order (分幕、出幕、悬念结局、季弧、瓶子集).

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Generate production subtitle artifacts from the final narration audio or final merged video using Volcengine Doubao ASR word timestamps. Use for local IndexTTS2 videos, Xiaohei page videos, talking-head delivery subtitles, SRT/VTT export, or whenever subtitle timing must match the final audio. Do not use script-length interpolation for final deliverables.

日本語の概要は準備中です。原文の説明を表示しています。