Profile functional potential of metagenomes using HUMAnN3 and similar tools. Use when obtaining pathway abundances, gene family counts, or functional annotations from metagenomic data.
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Profile functional potential of metagenomes using HUMAnN3 and similar tools. Use when obtaining pathway abundances, gene family counts, or functional annotations from metagenomic data.
日本語の概要は準備中です。原文の説明を表示しています。
Predict metagenome functional content from 16S rRNA marker gene data using PICRUSt2. Infer KEGG, MetaCyc, and EC abundances from ASV tables. Use when functional profiling is needed from 16S data without shotgun metagenomics sequencing.
日本語の概要は準備中です。原文の説明を表示しています。
Differential abundance and state analysis for cytometry data. Compare cell populations between conditions using statistical methods. Use when testing for significant changes in cell frequencies or marker expression between groups.
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ChIP-seq quality control metrics including FRiP (Fraction of Reads in Peaks), cross-correlation analysis (NSC/RSC), library complexity, and IDR (Irreproducibility Discovery Rate) for replicate concordance. Use to assess experiment quality before downstream analysis. Use when assessing ChIP-seq data quality metrics.
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End-to-end binder design using BindCraft hallucination. Use this skill when: (1) Designing protein binders with built-in AF2 validation, (2) Running production-quality binder campaigns, (3) Using different design protocols (fast, default, slow), (4) Need joint backbone and sequence optimization, (5) Want high experimental success rate. For backbone-only generation, use rfdiffusion. For QC thresholds, use protein-qc. For tool selection guidance, use binder-design.
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Guidance for SPR and BLI binding characterization experiments. Use when: (1) Planning binding kinetics experiments, (2) Troubleshooting poor/no binding signal, (3) Interpreting kinetic data artifacts, (4) Choosing between SPR vs BLI platforms.
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Analyze Stockbee-style Day 1 Episodic Pivot candidates from earnings, guidance raises, M&A, FDA/regulatory approvals, analyst actions, major contracts, product launches, short-squeeze catalysts, or theme/story events. Scores catalyst quality together with gap/range expansion, volume shock, neglect/revaluation context, liquidity, and risk to the EP-day low. Use when the user asks for EP candidates, episodic pivots, Day 1 catalyst trades, game-changing news reactions, delayed EP watchlists, or handoffs into PEAD monitoring.
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Expert guidance for systematic backtesting of trading strategies. Use when developing, testing, stress-testing, or validating quantitative trading strategies. Covers "beating ideas to death" methodology, parameter robustness testing, slippage modeling, bias prevention, and interpreting backtest results. Applicable when user asks about backtesting, strategy validation, robustness testing, avoiding overfitting, or systematic trading development.
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Options trading strategy analysis and simulation tool. Provides theoretical pricing using Black-Scholes model, Greeks calculation, strategy P/L simulation, and risk management guidance. Use when user requests options strategy analysis, covered calls, protective puts, spreads, iron condors, earnings plays, or options risk management. Includes volatility analysis, position sizing, and earnings-based strategy recommendations. Educational focus with practical trade simulation.
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Use when the user asks to "write SEO content", "draft a blog post / landing page", "update outdated content", or "fix traffic/ranking decay"; two modes — new drafts pages with keywords, headers, snippets, and evidence boundaries; refresh scores decay, prioritizes update work, and produces a republish plan with GEO guidance. Not for AI-citation/GEO readiness scoring — use geo-content-optimizer; not for publish-gate scoring — use content-quality-auditor. SEO文章写作/内容更新/排名恢复
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Use when the user asks to "build a B2B cold-outbound sequence", "design reply-triage branching", "plan a domain warmup / sending throttle", or "make my outbound CAN-SPAM / opt-in compliant"; produces a multi-step outbound sequence with reply-triage branches (positive / objection / referral / not-now / opt-out), a warmup + send-throttle ramp schedule, jurisdiction opt-in/CAN-SPAM guardrails (guidance, not legal advice), and a SEND S-dimension read. Not for B2C lifecycle flows — use email-sequence-designer; not for the consent record — use consent-registry; not for computing EQS — use email-quality-auditor. B2B冷启动外联序列/回复分流/域名预热
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Prevent destructive operations using Claude Code hooks. Three modes — cautious (warn on dangerous commands), lockdown (restrict edits to one directory), and clear (remove restrictions). Uses PreToolUse matchers for Bash, Edit, and Write.
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Audit connected MCP servers for token overhead, redundancy, and security. Use when sessions feel slow or before adding new MCPs.
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Best-practice authoring guidance AND a read-only policy gate for AWS Terraform generated by a migration skill. Load during any phase that writes a terraform/ directory — first as the "what to emit" posture rules + security-baseline spec, then after writing as the deterministic policy verdict. Read-only: it reports whether the generated Terraform passes; it never edits .tf files, never touches .phase-status.json, and never decides phase completion. Complements (does not replace) terraform fmt/init/validate.
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Startup-tailored AWS architecture advice for non-agent workloads, adjusted to the company's stage (pre-revenue through Series B+), team size, runway, and credits. Not for AI agents: deploying an AI agent on AWS, picking its runtime, or any agentic architecture is agent-advisor. Use when a founder wants guidance or a recommendation rather than code changes: which services to choose, how to plan or review an architecture, how to stretch credits and control cost, or how to prepare architecture for a fundraise or technical diligence. For an interactive discovery flow that scaffolds and writes the architecture into the codebase, use start-building-for-startups. Do not use for: writing or scaffolding code, factual AWS Activate / programs / credits lookups (see knowledge-base-for-startups), a single copy-paste prompt (see prompt-library-for-startups), or migration intent such as GCP-to-AWS, Azure-to-AWS, or Heroku-to-AWS (see the migration skills: `gcp-to-aws`, `azure-to-aws`, `heroku-to-aws`, `llm-to-bedrock`).
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Use when preparing your agent for production — IAM scoping, inbound auth (JWT, SigV4), secrets management, cold start optimization, session lifecycle, rate limiting, input validation, and quota guidance. Triggers on: "production checklist", "harden agent", "production ready", "secure agent", "inbound auth", "going live", "cold start optimization", "session lifecycle", "StopRuntimeSession", "quota", "throttling", "maxVms", "rate limit", "security audit of outbound API calls", "gateway target audit for production", "restrict who can call", "lock down endpoint", "only our app can call". Not for Cedar tool-restriction policies — use agents-connect. Not for quality measurement — use agents-optimize. Not for outbound credential storage or API key wiring — use agents-connect. Not for A2A agent-to-agent auth — use agents-build. Cold start observation and diagnosis (not optimization) routes to agents-debug.
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Establishes VPC peering connections between two VPCs for direct private network connectivity. Always use this skill when creating or managing VPC peering — it validates CIDR overlap, updates all route tables in both VPCs, configures DNS resolution, and provides security group guidance that are critical for correct connectivity.
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Provides connectivity, authentication, and troubleshooting guidance for Amazon RDS for SQL Server. Applicable when users ask about SSMS times out connecting from EC2, Cannot generate SSPI context with Windows auth, connect RDS SQL Server from Lambda with pymssql, auth_scheme shows NTLM instead of KERBEROS on ECS Fargate, SSM tunnel to RDS SQL Server from laptop, port 1433 security group, TrustServerCertificate=True for localhost tunnels, SPN MSSQLSvc, AWS Managed Microsoft AD, CNAME not RDS endpoint for Kerberos, tds_version='7.4', encryption='require', port-as-string for pymssql, Secrets Manager credential caching in Lambda, error 18456 login failed. Covers Python (pymssql, pyodbc), .NET (Microsoft.Data.SqlClient), Java (JDBC mssql-jdbc), Node.js (tedious), IAM auth via RDS Proxy, and VPC/ECS/EKS/Lambda deployment.
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Retrieves authoritative guidance on Amazon Timestream for InfluxDB across its supported engine variants. Applicable to any InfluxDB-on-AWS request including engine variant and licensing selection, provisioning and IAM, encryption at rest (AWS-owned and customer-managed KMS keys, key policies, and key lifecycle), schema design (tags vs fields, cardinality, HTTP/sensor/metric data modeling), migration from LiveAnalytics, Processing Engine plugins, connectivity (database endpoints and private or public access), and write/query errors.
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Provides authoritative compatibility checks, pricing estimates, connection troubleshooting, pre-warming guidance, and infrastructure mutations for Amazon Keyspaces (for Apache Cassandra). Covers LWT/batch operations, secondary indexes, materialized views, capacity modes, TTL, PITR, CDC, auto-scaling, multi-region keyspaces, UDTs, nodetool diagnostics parsing, SQL-to-Cassandra migration, and Cassandra-to-Keyspaces migration scenarios. Agents frequently produce incomplete or incorrect answers about Keyspaces feature support without this skill loaded.
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Provides authoritative guidance on Amazon Neptune Database and Neptune Analytics for graph, knowledge-graph, and relationship-heavy workloads — fraud detection / fraud rings, agentic memory / chatbot context across sessions, recommendations, identity resolution, Gremlin / openCypher / SPARQL queries, supernode / slow traversal, Neo4j to Neptune migration / APOC compatibility, Neptune Database vs Analytics engine selection, PageRank / community detection, GraphRAG, and connectivity from Lambda / EC2 / applications. Creates and modifies Neptune Database clusters/instances and Neptune Analytics graphs on explicit user confirmation; blocks destructive operations (delete, reset-graph, failover, major upgrade) and redirects to change-control.
日本語の概要は準備中です。原文の説明を表示しています。
KubeKey (kk) Usage Guide. For Kubernetes cluster lifecycle management including creation, scaling and deletion. Use this skill whenever the user mentions kubekey, kk commands, cluster setup, cluster management, adding nodes, deleting nodes, creating inventory files, configuring clusters, installing Kubernetes, or deploying K8s. Also use this skill when the user asks about inventory.yaml, config.yaml formats, CNI configuration, container runtime setup, offline installation, or artifact operations. Provides detailed guidance on kk subcommand usage, config file formats, inventory structure and common workflows.
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Configure this skill should be used when the user asks about "API contract", "api-contract.md", "shared interface", "TypeScript interfaces", "request response schemas", "endpoint design", or needs guidance on designing contracts that coordinate backend and frontend agents. Use when building or modifying API endpoints. Trigger with phrases like 'create API', 'design endpoint', or 'API scaffold'.
日本語の概要は準備中です。原文の説明を表示しています。
Execute use when you need to work with query optimization. This skill provides query performance analysis with comprehensive guidance and automation. Trigger with phrases like "optimize queries", "analyze performance", or "improve query speed".
日本語の概要は準備中です。原文の説明を表示しています。