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End-to-end shotgun metagenomics workflow from FASTQ to taxonomic and functional profiles, orchestrating controls/host depletion, Kraken2+Bracken classification, MetaPhlAn marker profiling, and HUMAnN functional profiling. Covers the controls-first ordering, why Kraken2 read counts are not abundances and MetaPhlAn cell fractions do not equal Bracken read fractions, and the consistent-pipeline framing. Use when profiling shotgun metagenomic samples end to end, or chaining classification, abundance, and function. For resistome see metagenomics/amr-detection; for strains see metagenomics/strain-tracking; for assembly see genome-assembly/metagenome-assembly.

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Build an animation from scratch, making the decisions in the order that determines whether it feels right — should it animate at all, what purpose, which tool, which properties, which curve and duration, how it interrupts, how it exits. Writes the implementation. Use when asked to animate something, add motion, make a component feel alive, or build a transition. For critiquing existing motion use review-animations; for auditing a whole codebase use improve-animations.

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Guide for implementing smooth, native-feeling animations using React's View Transition API (`<ViewTransition>` component, `addTransitionType`, and CSS view transition pseudo-elements). Use this skill whenever the user wants to add page transitions, animate route changes, create shared element animations, animate enter/exit of components, animate list reorder, implement directional (forward/back) navigation animations, or integrate view transitions in Next.js. Also use when the user mentions view transitions, `startViewTransition`, `ViewTransition`, transition types, or asks about animating between UI states in React without third-party animation libraries.

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Convert PDF to Markdown using opendataloader-pdf — extracts text, tables, headings, and images with correct reading order

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Video and audio processing capabilities for Flutter applications

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Generate an XLSX inventory report with stock levels, reorder points, unit costs, and total value formulas for purchasing teams.

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Generate a PDF packing slip and a PNG shipping label from the same order data in a single fulfillment workflow.

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Generate a packing slip PDF with order details, item list, and shipping address.

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Extract SKUs, product names, unit prices, availability, and minimum order quantities from a supplier catalog page.

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Extract shipper, consignee, order references, carton lines, weights, and package totals from packing lists.

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Spawns pedestrians that wander autonomously via WalkerAIController, and destroys them in the correct order. Pairs each walker.pedestrian.* with a controller.ai.walker, places them on the navigation mesh, and sends them to random navmesh points at walking speed. Use when the user asks to "spawn pedestrians/walkers", "add people/crowds", "make pedestrians walk around", or "remove the walkers". Reads the world settings first: if the world is already synchronous it never ticks it, and if it is asynchronous it makes it synchronous and drives the clock itself.

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Replays a CARLA .log recorded by the recorder — the whole run or a time window, at any speed, following a chosen actor, optionally regenerating sensors and restoring weather. Use when the user asks to "replay the recording", "play back the run", "watch it again in slow motion / at 2x", "follow vehicle N during replay", or "replay onto a different map".

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Queries the live CARLA world and resolves an entity description into a concrete actor id — find actors by type, role, or colour, or the single one nearest a point/actor; read the world snapshot; get static level bounding boxes by label; cast rays for semantic points; project a point to the ground; or (ros-topics) list the native ROS 2 topics each actor should be publishing and why any is silent. Identifies by stable attributes, not by any rank/order. Use when the user asks "what actors are here", "find the red prius", "the vehicle nearest the ego", "list the pedestrians", or needs a specific actor id for another skill.

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Creates and edits CARLA Leaderboard route files — placing waypoints and scenario trigger points interactively from the spectator camera (route_creator, scenario_creator), visualising an existing route in the simulator (route_displayer), summarising it as a table (route_summarizer), capturing the current weather in route format (weather_creator), sorting scenarios by route position (scenario_orderer) and converting 1.0-format routes to 2.x (route_bridge). Use when the user asks to "make a new route", "add a scenario to a route", "visualise/debug a route", "see what's in this route file", or "convert old leaderboard routes".

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Use when designing inclusive animations, addressing vestibular disorders and motion sensitivity, or ensuring animation accessibility compliance.

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Create pixelated borders, shadows, and effects for 8-bit retro components. Apply when building 8-bit styled UI components that need authentic pixel art aesthetics.

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Help a user talk through whether to change jobs, stay, or change something in their current work, starting from uncertainty, distress, a vague sense of stagnation, an offer in hand, a counter-offer from the current employer, or pressure from family or colleagues. Clarify what matters, separate deadlines that order the next steps from the decision itself, compare conditional options, and agree on a small next step and a time to revisit it. Use when someone asks whether they should quit, wonders whether leaving is just running away, has second thoughts after receiving an offer or being asked to stay, feels stuck because others say it would be a waste, wants to leave soon after joining, or wants advice before deciding to start a job search; do not make the final stay-or-leave decision, evaluate offer terms, plan the resignation, or predict hiring outcomes.

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Work out the order and the loose ends before turning down an offer or withdrawing from a selection process: whether the offer being kept is actually settled in writing, whether each decline is mid-selection, post-offer, or after acceptance, who besides the employer has to be told when the application came through an agent, a school recommendation, or a referral, which reply deadlines have passed, and what documents, expenses, or borrowed items are still outstanding. Use when a user holds more than one offer and needs to decline the rest, wants to withdraw from a process they are no longer pursuing, or has been asked to turn down other employers as a condition; do not use to decide whether to decline, to rule on what declining after acceptance means legally, or to send any message.

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Check, from the employer's side, what has been put in writing before a paid Otameshi Tenshoku assignment starts under gyomu-itaku: item by item whether the deliverable, delivery date, fee and how it is calculated, payment due date, who pays, out-of-scope work, revision limits, company support, rights, confidentiality and termination are stated in a contract, purchase order or email rather than in chat or by word of mouth, count the days from delivery to payment, and find pay tied to satisfaction or hiring, unpaid or unlimited revisions and one-sided termination. Use when a hiring manager, recruiter or small-business owner is about to offer terms to a candidate, has been asked by a candidate what is still unwritten, or needs the conditions written for a platform contract screen; do not use to judge whether the terms are lawful or the fee fair, to compute withholding or consumption tax, for employment contracts, or to sign, send or conclude the contract on the user's behalf.

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Assemble a Japanese shokumu-keirekisho (career history document) from an experience inventory, choosing between reverse-chronological, chronological, and skills-based layouts, mapping the target posting's requirements to the experiences that can support them, and drafting the summary, work history, skills, and self-PR so that each sentence stays within what its evidence supports and confidential details are abstracted. Use when a user wants to write or rebuild a whole shokumu-keirekisho for a specific posting or as a general version, or wants to decide which experiences to include and in what order; do not use to invent experience, numbers, titles, or qualifications, to predict whether it will pass screening, to fill in a rirekisho form, or to submit it anywhere.

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Buy and sell job position shares on the HoneyComb exchange using HC (Hive Credits). Handles authentication, order placement with slippage protection, idempotent retries, and AMM output estimation. Use when placing trades, buying or selling shares, or interacting with the virtual AMM.

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The default code-driven browser path for interactive browsing work: drives any browser from code with token-efficient a11y snapshots (57% smaller via compactSnapshot), ref-based clicks, coordinate control (CUA), viewport pinning, CAPTCHA solving (reCAPTCHA, Turnstile, hCaptcha, slider, OCR), Chrome MV3 APIs (tabs/bookmarks/history/downloads/topSites), network snoop (read API JSON instead of DOM), flight-recorder trace (jsonl + HAR + screenshots), OOPIF-aware snapshots, dialog policy, human handoff for login/OTP, device emulation, request routes, stealth via CloakBrowser as the default owned engine with zero exposed CDP ports, and an attached engine that drives the user's own signed-in browser through BrowserSkill (connectBrowserSkill, bskSnapshot, one-click onboarding via bskOnboard). MUST USE for interactive browser work: scraping, blocked/WAF/JS-rendered pages, logins, the user's logged-in sites and open tabs, extension popups, form filling, screenshots, web QA, CAPTCHAs. The ulw-research browsing lane runs on ultimate-browsing, not this skill.

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Loads, converts, and manipulates Hi-C contact matrices in cooler format (.cool/.mcool/.scool) and Juicer .hic, using cooler (Python + CLI), hic2cool, and hictk. Covers the single-resolution mcool URI (file.mcool::/resolutions/<bp>), the load-bearing divisive-vs-multiplicative weight-naming rule (KR/VC/VC_SQRT auto-divisive vs cooler's multiplicative weight), what survives .hic<->.cool conversion (FRAG matrices and norm vectors do not), raw-vs-balanced coarsening, the .pairs upper-triangle/chromsize-order contract, and chrom-naming/bin-table provenance. Use when loading a cooler, converting .hic to .mcool, selecting a resolution, building a cooler from pairs or a matrix, coarsening/zoomifying, importing Juicer norm vectors, or debugging all-NaN balanced matrices and chr1-vs-1 empty fetches.

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Orders and orients assembled contigs into chromosome-scale scaffolds from long-range linking data, inserting N-gap spacers (adds no sequence). Covers Hi-C/Omni-C scaffolding (YaHS, SALSA2, 3D-DNA/Juicer), Hi-C read-mapping prerequisites (map each end separately, no mate rescue, dedup, enzyme-aware), reading the contact map for misjoins/inversions/false-duplications, manual curation in Juicebox/PretextView (the VGP/DToL standard), reference-guided scaffolding (RagTag) and its karyotype-erasure hazard, genetic-map (ALLMAPS) and Bionano optical-map integration, chimera-breaking before scaffolding, gap-filling, and telomere/contig-vs-scaffold-N50 QC (tidk). Use when turning contigs into chromosomes with Hi-C, integrating a linkage map or optical map, choosing a scaffolder by available linking data, or judging whether a chromosome-scale assembly is trustworthy.

日本語の概要は準備中です。原文の説明を表示しています。