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Create an offline HTML explanation of a code change (diff, PR, branch) or of a topic, system, or question. Use when a visual walkthrough document is requested.

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Workflow skill for KiCAD PCB layout and routing via MCP tools. Triggers on: "layout the board", "route traces", "PCB", "place footprints", "copper pour", "board outline", "differential pair", "board setup", "track width", "via", "zone", "design rules", "stackup", "silkscreen".

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Build, test, and drive iOS apps in the Simulator with 30 token-efficient scripts. Use when asked to build or test an Xcode project, launch an app on a simulator, tap/type/swipe through its UI by accessibility label, take screenshots or visual diffs, audit accessibility, read logs or diagnose hangs, or manage simulators (boot, erase, permissions, location, push, appearance). macOS only; needs Xcode, Python 3.12+ and idb.

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Use when articulating and differentiating the theoretical contribution of an Academy of Management Review (AMR) manuscript — showing precisely what is NEW versus prior theory and why it matters. Frames the contribution; it does NOT build the theory (amr-theory-development) or check its internal logic (amr-data-analysis).

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Review generated or changed test code against universal testing rules before it ships. Best used reactively after an agent writes, edits, generates, or refactors tests, before presenting, committing, or merging them. Use for pytest (test_*.py, *_test.py), PHPUnit/Pest (*Test.php), Jest/Vitest (*.test.ts, *.spec.js), Go (*_test.go), files under tests/, __tests__/, or spec/, and review requests like 'write tests for X', 'add tests', 'test this', 'review these tests', or PR diffs containing tests. Can also guide test writing when explicitly invoked before the work. This skill is the quality gate that prevents AI-generated test bloat. DO NOT USE for production or implementation code review (use clean-code-guard), CI or test-runner configuration, running or debugging tests, or general architecture discussion.

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Detect buying signals across TAM companies and watchlist personas. Three-phase architecture: (1) free diff-based signals from existing data (headcount growth, tech stack changes, funding rounds), (2) Apify-powered signals (job postings, LinkedIn content analysis, profile changes), and (3) post-processing with dedup, scoring, and lead status updates. Writes signals to Supabase signals table for downstream activation.

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Generate detailed, differentiated content briefs at scale. Each brief includes SERP analysis, competing page breakdown, unique angles from real customer language (reviews, Reddit), internal linking plan, and SERP feature targets. Batch mode produces 10-50 briefs in one run. Crushes generic "keyword density" briefs from tools like Surfer or Clearscope.

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Generate a personal voice guide for X (Twitter) and/or LinkedIn by scanning a user's past posts and iteratively refining with sample-and-feedback loops. Produces a structured markdown voice guide that sibling skills (create-x-content, create-linkedin-content) consume to draft in-voice posts. Different from brand-voice-extractor, which analyses company blogs/landing pages — this skill is for personal social voice.

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Turn a brand's facts, a campaign brief and the product into a one-minute Meta ad strategy with its reasoning — goal, audience, objective and placements, budget split, how many ads and how different they must be (generate 3x so the user can choose), the test frame, the success metric, and safety rules for the user to accept. Decides what launch-meta-ad-campaign then executes, and re-reads the strategy after a deep check to show which assumption changed. Use it after intake and before any creative is generated or any campaign is built.

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Research competitors, analyze their messaging, and generate a positioning document with category definition, differentiation claims, value propositions, and proof points. Chains web research, competitor site analysis, and review mining to produce a positioning doc ready for website copy and sales deck use. Use when a product marketing team needs to define or refresh positioning ahead of a launch, rebrand, or competitive shift.

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Drive and verify an Electron or Tauri desktop app from the inside, including the main-process and Rust IPC calls a browser tool cannot see. Use when a desktop app needs testing, when a feature works in the browser but not in the packaged app, when an IPC or invoke call needs proving, when a desktop screenshot or visual diff is wanted, or when you need a headless run of a desktop UI in CI.

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Use this skill to run multiple Simulink simulations in parallel with parsim, batchsim, and DesignStudy. TRIGGER when: user asks for parameter sweep, sweeping/varying parameters across simulations, Monte Carlo study, batch simulation, running multiple simulations, parallel simulations, large-scale multiple simulations, any task involving repeated simulations with different parameter values, user mentions having an existing script/function for parameter sweeps, or user wants to migrate from parfor/set_param/for-loop patterns to recommended workflows.

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Build reactive/event-driven C# with R3 (Cysharp's modern reimplementation of Reactive Extensions). Covers the Observable<T>/Observer<T> model, the OnErrorResume error contract, async dispatch with AwaitOperation, Task/IAsyncEnumerable integration, TimeProvider/FrameProvider scheduling, the concurrency contract, and how R3 differs from System.Reactive (Rx.NET).

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Balances Hi-C contact matrices (ICE via cooler.balance_cooler, KR/SCALE/VC context), computes distance-decay expected with cooltools (expected_cis per-diagonal P(s), expected_trans scalar), builds observed/expected (O/E) matrices, and diagnoses polymer state from the P(s) log-derivative. Covers the within-matrix-vs-cross-sample distinction (balancing is NOT a normalizer), the equal-visibility assumption that CNV/aneuploidy violates (use raw counts for copy-number), cis-only balancing, mad_max/blacklist masking before balancing, multiplicative cooler weights vs divisive juicer weights, and the resolution-vs-depth budget. Use when balancing a .cool/.mcool, computing expected or P(s), making O/E matrices for compartments/loops, deciding ICE vs KR vs SCALE, choosing a resolution for a given depth, or troubleshooting NaN/all-NaN balanced matrices; route cross-sample comparison to hic-differential.

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Assembles microbial-community sequencing into metagenome-assembled genomes (MAGs) with metaFlye (ONT), metaSPAdes/MEGAHIT (Illumina), and hifiasm-meta/metaMDBG (PacBio HiFi), then recovers genomes via multi-binner consolidation (MetaBAT2, MaxBin2, CONCOCT, SemiBin2, VAMB -> DAS_Tool) and QCs them against MIMAG with CheckM2, GUNC, and GTDB-Tk. Covers why a metagenome is not a genome (uneven coverage, micro-diversity, strain collapse to consensus), differential-coverage binning, co-assembly vs per-sample, the rRNA-operon collapse that fails short-read MAGs, and strain resolution with inStrain. Use when reconstructing genomes from a microbiome, soil, ocean, or gut community, recovering MAGs, or resolving strain-level variation.

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Detects focal chromatin loops (point interactions / corner-dots) in balanced Hi-C and Micro-C contact maps and aggregates/validates a loop set. Covers de-novo calling with cooltools dots (HiCCUPS-style 4-background local enrichment with lambda-chunked FDR), chromosight (template-correlation), and Mustache (scale-space blob detection); aggregate peak analysis (APA) via cooltools pileup for confirmation; the depth/resolution prerequisite (de-novo needs ~5-10kb resolution = hundreds of millions to billions of valid pairs); consensus across callers and convergent-CTCF support as validation; and differential loops via union anchors plus chromosight quantify. Use when calling chromatin loops or dots from a cooler, deciding whether a map is deep enough to call de-novo vs running APA on known CTCF/cohesin anchors, building an aggregate peak pileup, comparing loops across conditions, or validating loop calls. For HiChIP/PLAC-seq/PCHi-C protein-anchored data use FitHiChIP/MAPS, not dots.

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Calls m6A peaks from MeRIP-seq / m6A-seq paired IP-vs-input data using exomePeak2 (transcript-aware, GC-bias-corrected Poisson GLM), MeTPeak (HMM over sliding windows), MACS3/MACS2 with --nomodel --broad --keep-dup all (genome-wide broad alternative), and DRACH motif enrichment via HOMER or ggseqlogo as a sanity check (NOT a filter). Covers BED12 vs narrowPeak output, exonic vs intronic peak handling, multi-tool reconciliation (intersection vs union), the m6A-vs-m6Am ambiguity at 5'UTR peaks that antibody methods cannot resolve, and orthogonal validation (miCLIP/GLORI/m6A-SAC-seq/m6Anet). Use when calling peaks from paired IP/input genome BAMs, choosing exomePeak2 (transcript-aware default) vs MACS3 (broad genomic) vs MeTPeak (HMM-smoothed low-coverage), confirming DRACH enrichment as a sanity check on the peak set, reconciling differing peak sets across tools, validating MeRIP peaks against single-base methods, interpreting 5' peaks where m6Am contamination is possible, or recommending a consensus strategy.

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Build weighted gene co-expression networks to identify modules of co-regulated genes, relate them to phenotypes, and find hub genes using WGCNA, hdWGCNA, MEGENA, CEMiTool, and Gaussian graphical models. Covers signed-network choice, soft-threshold selection, module preservation, and the marginal-vs-partial-correlation distinction. Use when finding co-expression modules, identifying hub genes, relating gene networks to clinical or experimental traits, or building single-cell co-expression networks. For directed TF-target inference see scenic-regulons and grn-inference; for condition rewiring see differential-networks.

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Quality control for flow, spectral, and mass cytometry - time-based anomaly cleaning (flowAI, flowCut, PeacoQC, flowClean), margin/boundary event removal, signal-drift detection, dead-cell exclusion, CyTOF Gaussian/DNA/event-length checks, instrument calibration/standardization (MESF, CS&T, peak-2), and batch-level outlier flagging. Use when assessing acquisition quality, choosing a cleaning tool, ordering QC relative to compensation, deciding margin removal before density-based steps, or flagging problematic samples before clustering or differential analysis.

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Infers person-to-person transmission from pathogen genomes using outbreaker2, TransPhylo, phybreak, BadTrIP, SCOTTI, BEASTLIER, and SNP-distance / cluster-picker approaches (HIV-TRACE for HIV; transcluster). Defines outbreak clusters using pathogen-specific SNP thresholds (NOT a universal cutoff -- TB <=12 SNPs; MRSA <=15; C. difficile <=2; Klebsiella <=21), models within-host diversity and transmission bottlenecks, integrates contact-tracing data, distinguishes generation from serial interval, and attributes source via Bayesian source attribution (islandR). Use when investigating outbreaks for who-infected-whom, defining SNP-cluster outbreak definitions, accounting for unsampled intermediates, choosing between outbreaker2 (rich epi data) and TransPhylo (genomic-only after a dated phylogeny), running source attribution between host populations, calling HIV-TRACE thresholds appropriate to the local subtype, or distinguishing recent transmission from reactivation in TB or chronic HIV.

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Designs genomics experiments so technical nuisance variation (batch, lane, plate, flow cell, operator, reagent lot, processing day) is balanced against the biological variable of interest and therefore estimable rather than confounded, using constrained sample-to-batch assignment (designit, OSAT), the confounder/mediator/collider distinction, and the principle that no post-hoc correction recovers a fully confounded design. Covers detecting hidden batches with surrogate variable analysis, a decision table for downstream correction (ComBat-seq, RUVSeq, SVA) whose execution is deferred to differential-expression/batch-correction, and reproducibility metadata. Use when assigning samples to sequencing batches/lanes/plates, avoiding batch-condition confounding, deciding whether a design is salvageable by correction, choosing a correction method, or estimating the number of hidden batches. For the experimental unit, randomization, and blocking concepts see experimental-design/randomization-blocking.

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Handles batch effects in bulk RNA-seq via design-matrix inclusion (the correct path for DE), ComBat/ComBat-seq for visualization, SVA for unknown latent factors, RUVSeq for negative-control-gene-anchored unwanted variation, and limma::removeBatchEffect for plotting only. Encodes the Nygaard 2016 cardinal sin against testing on a batch-corrected matrix, the choice between SVA/RUVg/RUVs/RUVr, the confounding non-identifiability problem, the single-cell boundary (Harmony/MNN are NOT for bulk), and the Goh 2017 harmonization critique. Use when designing a DE analysis with batch structure, troubleshooting batch-dominated PCA, choosing ComBat vs ComBat-seq, handling unknown batch via SVA, integrating across studies, or deciding when (rarely) to subtract batch.

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Runs JACKS (Joint Analysis of CRISPR/Cas9 Knockout Screens; Allen et al 2019 Genome Research) which models per-sgRNA log-fold-change as the product of a treatment-dependent gene-essentiality term and a treatment-independent guide-efficacy term. Covers the Bayesian decomposition math, the hierarchical efficacy prior shared across screens performed with the same library, when JACKS outperforms MAGeCK (multi-screen joint analysis, libraries with broad efficacy variance) and when it does not (single screen, novel libraries with no prior efficacy), library-reuse efficacy transfer, downstream essentiality interpretation, and the 2.5x sample-size reduction enabled by efficacy-aware testing. Use when running multiple screens with the same library, when guide-level noise is suspected to dominate per-gene signal, when reusing published essentiality reference screens for efficacy priors, or when comparing screens performed across cell lines that share library but differ biologically.

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Creates DE-specific diagnostic and result visualizations using DESeq2/edgeR built-in functions and lightweight ggplot2 wrappers. Covers MA plot (with the shrunken-LFC compression effect), volcano (with the apeglm caveat that p-values are unchanged), PCA on VST/rlog (never raw counts), sample distance heatmaps, top-DE-gene heatmaps with the row-scaling trap, dispersion / BCV plot interpretation, p-value histogram diagnostics, plotCounts for individual genes, blind=TRUE vs FALSE rationale, and the n=3 visualization stake. Use when generating DE diagnostic plots, choosing VST vs rlog for visualization, troubleshooting suspicious plot patterns (shifted MA cloud, batch-dominated PCA, anti-conservative p-value histogram), or building a standard QC figure panel.

日本語の概要は準備中です。原文の説明を表示しています。