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PowerPoint slide deck generation and management using python-pptx with YAML-driven content and styling

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读取 Word/WPS 审阅后的 docx，把批注（comments）与修订（track changes）提取成可逐条裁决的 markdown 对账表或 JSON。触发场景：对方批注完的合同/协议/书稿/报告回来了要读意见；「提取 docx 批注」「审阅意见对账」「读一下修订」「谁批了什么、批在哪」；WPS 云文档/Word 在线协作 导出的审阅稿；收到带修订模式改动的回稿要看对方改了什么。核心保证：修订感知——python-docx 裸读会漏掉修订插入（w:ins）的内容、认不出整段删除（w:del），本 skill 走 OpenXML SDK 引擎 不漏。只读，不改对方文件。边界：生成/排版 docx → docx-creator 或 minimax-docx；PDF 批注不在范围。

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Configures and runs LLM evaluation using Promptfoo framework. Use when setting up prompt testing, creating evaluation configs (promptfooconfig.yaml), writing Python custom assertions, implementing llm-rubric for LLM-as-judge, or managing few-shot examples in prompts. Triggers on keywords like "promptfoo", "eval", "LLM evaluation", "prompt testing", or "model comparison".

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Convert binary documents (PDF, DOCX, XLSX, PPTX, HTML, EPUB, images) to clean LLM-friendly Markdown using Microsoft's `markitdown` Python tool. Use when a user attaches a binary file and you need to read its contents.

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通用 Web API 数据采集、Node.js/Python 协议调用与前端签名分析。 使用 Camoufox MCP 捕获请求、调试 Hook、分析混淆/JSVMP/WASM， 交付可验证的采集程序，支持分页、断点续采和接口改版回归。 用于自有或已授权接口；微信小程序 wxapkg 解包使用对应小程序技能。

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Generate a Python code skeleton from an experiment blueprint

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Anthropic Claude API patterns for Python and TypeScript. Covers Messages API, streaming, tool use, vision, extended thinking, batches, prompt caching, and Claude Agent SDK. Use when building applications with the Claude API or Anthropic SDKs.

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Settle what domain terms mean per context, and which repository conventions or language standards (Go, Python) apply. Use when: names disagree or a rename is proposed.

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Create animated flow diagrams from articles, workflow notes, architecture sketches, or process descriptions using a JSON specification and a local Python/Pillow renderer. Use when the user wants to turn source material into a static PNG + animated GIF diagram.

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INVOKE THIS SKILL when setting up a new project or when asked about package versions, installation, or dependency management for LangChain, LangGraph, LangSmith, or Deep Agents. Covers required packages, minimum versions, environment requirements, versioning best practices, and common community tool packages for both Python and TypeScript.

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Stitch video segments with ffmpeg concat, xfade, overlay, audio mux, and export settings, and trim a source into named clips at exact windows (trim_clips.py). Ships montage.py, a free montage assembler (python3 + ffmpeg, no keys). It takes a JSON EDL of clips and stills, normalizes them and hard-cuts them in order, burns captions from an SRT, a cue list or word timings, and lays a VO over a music bed that ducks under it, mastered to -14 LUFS.

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Assemble an absurdist animated-explainer video ad (~38s, 9:16) from per-scene i2v clips + their measured VO windows — retime each clip to its VO, re-encode every segment to identical 30fps/libx264/yuv420p so the concat demuxer never drops frames, concat, build a REAL-product PIL end card (never AI) held static, mix VO (loudnorm I=-14) under music (loudnorm I=-26, volume 0.62, amix normalize=0), and burn libass captions last. FREE deterministic assembly (bash-free, Python + ffmpeg + PIL); the recipe supplies the clips, VO, music, product photo, palette, and caption table and gates the paid keyframe/clip/VO/music calls to their own capabilities. Use for the absurdist-explainer format.

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Assemble an animated character-explainer video ad (any art style — Pixar-style 3D, claymation, anime, paper-cut, felt) (~77s, 9:16) built on an "N types of X" listicle spine — a recurring human protagonist plus a locked cast of N persona characters, one per list item. Given the per-scene i2v clips + a per-scene target-duration table + a narration track, it trims each clip to its scene window, re-encodes every segment to identical 1080x1920/30fps/libx264/yuv420p (decrease+pad, never crop) so the concat demuxer never drops frames, concats, and muxes audio — in RESTYLE mode the source ad's VO+music mix is reused verbatim, in ORIGINAL mode fresh per-scene VO (loudnorm I=-14) is mixed under an optional music bed (loudnorm I=-26). A static-still fallback loops a scene's keyframe when its clip is missing/failed, so the master always assembles; libass captions are burned last. FREE deterministic assembly (Python + ffmpeg, no bash, no paid calls); the recipe supplies the clips, keyframes, VO or source audio, and caption table and gates the paid cast-anchor/keyframe/Kling-i2v/VO/music calls to their own capabilities. Use for the 3d-character-explainer listicle format.

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Assemble a myth-vs-fact kinetic-typography explainer video ad (≈29.5s, 9:16) from N myth/fact pairs + hook / turn / punch copy + palette + a brand end-card PNG + a VO track — a hook, 3 red-strike MYTH cards that flip to teal-check FACT cards (per-line strikethrough that crosses EVERY wrapped line), a "what actually works" turn, an optional proof reveal, a punch line, and a static end card. DETERMINISTIC assembly with ZERO AI-gen visuals — HTML hyperframes rendered frame-exact via Playwright (`window.renderAt(t)`, animation a pure function of beat-local time), Whisper beat-snap to VO word onsets, concat at a uniform fps, karaoke `.ass` captions burned last (suppressed on the proof + end-card beats), and a VO + optional music mix (music −20 dB, `amix normalize=0`, tail fade). FREE (Python + Playwright + ffmpeg); the recipe supplies the copy / palette / end-card / VO and gates the paid VO / music / Whisper calls to their own capabilities. Use for the myth-vs-fact format.

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Extracts cfDNA fragmentomics features (DELFI genome-wide short/long ratios, WPS nucleosome positioning, Griffin GC-corrected accessibility profiles, end-motifs/MDS, OCF) for cancer detection and tissue-of-origin from plasma WGS. Centers on the nuclease-footprint reframe (every feature re-reads one nucleosome object), the mandatory GC correction, and the cross-protocol non-comparability that breaks naive classifiers. Runs FinaleToolkit (real CLI/Python, MIT) and the Griffin Snakemake pipeline; DELFI is a method, not a package. Use when deriving fragment-based signal from cfDNA, choosing a feature family for detection vs subtyping, or diagnosing why a fragmentomic model failed validation.

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Performs set operations on genomic intervals - intersect (-wa/-wb/-wo/-wao/-loj/-c/-v/-u), subtract (-A), merge (-d, -c/-o), complement, cluster, multiinter, unionbedg, map, and groupby - with bedtools (CLI) and pybedtools/pyranges/bioframe (Python). Covers the sorted-input contract and the -sorted chromosome-order footgun, reciprocal/fractional overlap (-f/-F/-r/-e) and the A-vs-B asymmetry, -split for spliced/BED12/BAM features, and jaccard/fisher as mechanics only. Use when finding overlapping or unique regions between BED/peak/feature files, building consensus peaksets, removing blacklisted regions, transferring annotation values onto intervals, or computing interval-set similarity; route overlap-significance testing to overlap-significance.

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Reads, queries, and writes bigWig indexed binary signal tracks (coverage, fold-change, conservation, methylation-rate) with pyBigWig (Python) and the UCSC Kent tools (bedGraphToBigWig, bigWigToBedGraph, bigWigInfo, bigWigSummary, bigWigAverageOverBed) and deepTools (multiBigwigSummary, computeMatrix, bigwigCompare). Covers the central trap that a wide query returns a precomputed zoom-level summary (by default the mean, which annihilates narrow peaks) not per-base data, when exact=True/values() is mandatory, the NaN-not-zero gap-handling fork, choosing mean vs max vs sum vs coverage by biological question, and the sorted-bedGraph plus chrom.sizes build requirement. Use when extracting signal at regions, computing mean signal per gene/peak, building a browser track from bedGraph, comparing tracks, or building TSS/gene-body metaprofiles.

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Loads, converts, and manipulates Hi-C contact matrices in cooler format (.cool/.mcool/.scool) and Juicer .hic, using cooler (Python + CLI), hic2cool, and hictk. Covers the single-resolution mcool URI (file.mcool::/resolutions/<bp>), the load-bearing divisive-vs-multiplicative weight-naming rule (KR/VC/VC_SQRT auto-divisive vs cooler's multiplicative weight), what survives .hic<->.cool conversion (FRAG matrices and norm vectors do not), raw-vs-balanced coarsening, the .pairs upper-triangle/chromsize-order contract, and chrom-naming/bin-table provenance. Use when loading a cooler, converting .hic to .mcool, selecting a resolution, building a cooler from pairs or a matrix, coarsening/zoomifying, importing Juicer norm vectors, or debugging all-NaN balanced matrices and chr1-vs-1 empty fetches.

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Handles BED-format genomic intervals (BED3 through BED12, narrowPeak/broadPeak) and the coordinate-system substrate the whole interval category rests on, with bedtools (CLI) and pybedtools/pyranges/pandas (Python). Covers the 0-based half-open vs 1-based-closed convention boundary and the start-1/end-unchanged conversion, the silent failures (chrom-name mismatch, CRLF, lexicographic-vs-version sort under -sorted), genome/chrom.sizes generation, sorting contracts, BED12 block invariants, validation, makewindows, cross-assembly liftover (liftOver/CrossMap), and BED<->VCF/BAM/FASTA conversion. Use when reading, creating, validating, sorting, lifting between genome builds, or converting interval files, preparing inputs for bedtools/tabix/bigBed, or debugging an off-by-one or empty-overlap result.

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Reads, inspects, and writes Flow Cytometry Standard (FCS) files from conventional, spectral, and mass cytometry (CyTOF), and parses FlowJo/Cytobank/Diva workspaces. Covers FCS 2.0/3.0/3.1/3.2 internals ($PnE linear-vs-log, $DATATYPE, $SPILLOVER vs SPILL vs $COMP, $TIMESTEP), channel/parameter metadata, the silent linearize/truncate defaults, and R (flowCore, flowWorkspace, CytoML) plus Python (FlowKit, readfcs) readers. Use when loading flow or mass cytometry data, mapping detector channels to antibodies, extracting the event matrix, choosing a reader, or bridging FCS to the scanpy/AnnData ecosystem before preprocessing.

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Delimits putative species boundaries from molecular data within the de Queiroz 2007 unified-lineage framework using ASAP (Puillandre 2021 successor to ABGD), mPTP C++ (Kapli 2017 successor to bPTP; bPTP is Python NOT R), GMYC single/multi-threshold (Pons 2006; Fujisawa 2013), multilocus BPP v4 with prior calibration from data (NOT defaults; Yang 2015), SNAPP + BFD* for SNP delimitation, DELINEATE (Sukumaran 2021) speciation-process modeling to address Sukumaran & Knowles 2017 PNAS critique that MSC delimits structure not species, integrative-taxonomy congruence (Padial 2010; Carstens 2013), Dsuite for introgression testing before sister claims (Malinsky 2021), and Meyer & Paulay 2005 barcoding-gap-absence caveat. Use when delineating species from DNA barcoding data, resolving cryptic complexes, choosing among ASAP/mPTP/BPP/DELINEATE, calibrating BPP priors, distinguishing introgression from ILS, or applying the Sukumaran-Knowles oversplitting correction.

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Build circular genome visualizations using circlize (R), pyCirclize (Python), or Circos (Perl CLI) with ideogram tracks, multi-data tracks (scatter, histogram, heatmap), chord/link arcs for interactions, and explicit circos.clear() between plots. Covers when circular is appropriate vs when Cartesian wins (Cleveland-McGill 1984), karyograms, and chromosome adjacency in chord diagrams. Use when adjacency on the circle conveys meaning — chromosome-level overview, structural variants, Hi-C interactions, cross-genome comparisons.

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Bulk-query Ensembl BioMart (and other BioMart instances) for cross-database ID mapping, gene/transcript/exon coordinates, and ortholog tables. Use when batch-converting Ensembl IDs to other namespaces (HGNC, RefSeq, UniProt, Entrez), pulling gene coordinate tables for thousands of genes, building ortholog wide-tables across species, or replacing slow Ensembl REST loops with one-shot bulk export. Encodes BioMart's XML query format, R biomaRt vs Python pybiomart trade-off, mart-vs-dataset hierarchy, and the URL endpoint that's BioMart-specific (separate from rest.ensembl.org).

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Build publication-quality figures with matplotlib using the object-oriented Figure/Axes API, constrained_layout, rcParams customization, TrueType (Type-42) font embedding for journal submission, and CVD-safe palettes. Covers seaborn integration, common chart types, axis formatting, and the small gotchas that distinguish reproducible matplotlib from notebook scratch. Use when producing publication figures in Python — RNA-seq scatter, single-cell embeddings, generic biological plotting.

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