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概要と使いどころ
Use when running the final pre-submission preflight for American Economic Review: Insights (AER: Insights) via the AEA/ScholarOne submission system — the word-count and exhibit-count gates, abstract limit, fees, author metadata, declarations, and the AEA Data and Code Availability Policy. Final checks; it does not draft content.
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Use when polishing the prose of an Advanced Materials manuscript for Wiley house style, concision, precise materials nomenclature and units, and readability across materials subfields. Polishes language; does not restructure the advance or manage the length budget.
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Use when running the final pre-submission preflight for an Advanced Materials manuscript — article type, format, files, Supporting Information, TOC graphic, ORCID, and metadata for the Wiley submission system. Verifies submission readiness; does not write content.
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Use when designing or auditing ACM CCS experiments, attack demonstrations, adaptive-attack defense evaluations, security measurements, baselines, overhead and cost reporting, ablations, and claim-to-evidence fit, with emphasis on evidence that survives an adversarial program committee rather than leaderboard wins.
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Use when revising an ACM CCS paper for a precise threat model, calibrated attack/defense claims, 12-page ACM sigconf compression, double-blind wording, adaptive-evaluation honesty, and the security house style that survives an adversarial SIGSAC program committee.
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Use when preparing an accepted ACL main-conference or Findings paper for camera-ready, covering the extra content page, de-anonymization and acknowledgements, AI-assistance disclosure, keeping the Limitations section, ACL Anthology metadata and CC BY 4.0 publication, meta-review-driven edits, and presentation-mode logistics.
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Use when drafting an ACM CCS rebuttal during the HotCRP author-response window, covering threat-model defenses, adaptive-attack objections, ethics and disclosure questions, anonymity constraints, adversarial reviewer pushback patterns, and decision-focused replies aimed at the meta-reviewer.
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Use when revising an AAAI manuscript for broad-AI-audience fit, a first-page contribution statement legible to non-specialist Phase-1 reviewers, two-column readability, concise novelty claims, reproducibility-checklist alignment, hedged limitations and ethics, and policy-aware framing of AI-system and capability claims.
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Use when preparing an accepted AAMAS paper for the IFAAMAS open-access camera-ready, covering author de-anonymization, proceedings metadata, two-column reflow of game figures and learning curves, resolving rebuttal-stage promises, registration, in-person presentation, and the public code and environment release for a multiagent paper.
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行业情报雷达：多源扫描(X/即刻/V2EX/HN) → 关键词过滤 → 趋势检测 → 每日情报简报。触发词：行业情报、竞品监控、热点扫描、情报雷达
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Check and improve your brand's visibility across AI search engines (ChatGPT, Perplexity, Gemini, Grok, Claude, DeepSeek). Set up tracking, run visibility analyses, audit your website for AI readability, and get actionable recommendations. Uses the npx goose-aeo@latest CLI.
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Track product champions for job changes and qualify their new companies against ICP. Takes a CSV of known champions (with LinkedIn URLs), creates a baseline snapshot via Apify enrichment, then detects when champions move to new companies. Scores new companies on a 0-4 ICP fit scale. Outputs a downloadable CSV of movers with qualification verdicts.
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Extract competitor and customer intelligence from any company's landing page HTML. Discovers tech stack, analytics tools, ad pixels, customer logos, SEO metadata, CTAs, hidden elements, and more. No API keys required.
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Render a two-host podcast ad from a brand config and dialogue script. Includes the working planner, full-frame and split-screen assembly, captions from measured character timings, brand end card, approved paid-step adapters, and 65 quality-check falsification cases. Use for short conversational ads with two stable hosts in one room.
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Building or editing Simulink (.slx) models that stream signals in frames using DSP System Toolbox — audio, vibration, radar, comms. Use when the prompt names a Simulink block (Discrete FIR Filter, Buffer, Unbuffer, Rate Transition, Downsample, Upsample, Sample-Rate Converter, FIR Decimation, Time Scope, Spectrum Analyzer, From Multimedia File) or an action (frame-based processing, InputProcessing, buffering, overlap, windowing, STFT, short-time Fourier, decimate, downsample, upsample, resample, multirate, anti-aliasing, tunable filter). Prevents silent numerical errors from sample-vs-frame mismatch, wrong block choice, or missing anti-aliasing.
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Sanitize and anonymize a Simulink model for external sharing. Use when stripping proprietary content, anonymizing block/signal names, removing metadata, or creating a shareable reproduction model for bug reports. Do NOT use for simply copying or sharing a model internally.
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Assembles genomes de novo from noisy long reads (Oxford Nanopore R9/R10/Dorado, PacBio CLR) with Flye (repeat graph), Canu (correct-trim-assemble OLC), NextDenovo, Shasta, Raven, wtdbg2, or miniasm, and reconciles bacterial assemblies into a consensus with Trycycler/Autocycler. Covers matching the input flag to the basecaller era (--nano-hq vs --nano-raw), why a raw long-read assembly is contiguous but low-QV and not finished until polished, haplotig false-duplication and purge_dups, coverage and read-N50 as non-substitutable inputs, and mid-read adapter de-chimerization. Use when assembling a bacterial or eukaryotic genome from ONT or PacBio noisy reads, choosing a long-read assembler, or diagnosing an over-collapsed or duplicated assembly. For PacBio HiFi use hifi-assembly instead.
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Discovers, classifies, filters, and quantifies full-length transcript isoforms from PacBio Iso-Seq/Kinnex (HiFi) and Oxford Nanopore (cDNA/direct-RNA) long reads, using the isoseq+pigeon pipeline, SQANTI3, and ONT tools (IsoQuant, FLAIR, Bambu, StringTie2). Covers why a novel isoform is an artifact until proven otherwise (RT template-switching, intra-priming, and 5' degradation manufacture junctions and truncations), the SQANTI3 structural categories and their trust order, the Kinnex skera-split step, orthogonal CAGE/poly-A/short-read-junction validation, and why long-read isoform quantification needs EM. Use when building a full-length isoform catalog, classifying/filtering long-read transcripts, running Iso-Seq or ONT cDNA/dRNA analysis, or judging novel-isoform reliability.
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Calls germline small variants (SNPs and indels) from Oxford Nanopore and PacBio HiFi long reads with Clair3, a two-stage (pileup + full-alignment) deep-learning caller, selecting the chemistry- and basecaller-version-matched model, enabling read-based phasing, and benchmarking against GIAB with stratification. Covers why the model string is the experiment (no auto-detection, silent degradation on mismatch), why ONT homopolymer/STR indels are the residual error whole-genome F1 hides, and the somatic/trio/RNA boundary to the ClairS/Clair3-Trio family. Use when calling germline SNVs/indels from ONT or HiFi BAMs, choosing a Clair3 model, phasing variants, or benchmarking long-read calls.
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Decides how to preprocess plasma cfDNA sequencing data so the recoverable signal survives - library-prep-aware fragment expectations (dsDNA vs ssDNA/adaptase prep), UMI/duplex consensus with fgbio (ExtractUmisFromBam, GroupReadsByUmi --strategy paired for duplex, CallMolecularConsensusReads vs CallDuplexConsensusReads, FilterConsensusReads min-reads "total s1 s2"), the align->group->consensus->RE-align ordering, and the cfDNA dedup trap where naive coordinate dedup collapses nucleosome-coincident independent molecules. Covers when single-strand consensus suffices vs when duplex is mandatory, the singleton/sensitivity tax at low input, and reading the insert-size histogram as a pre-analytical QC instrument. Use when processing plasma cfDNA reads before fragmentomics, ctDNA mutation calling, or tumor-fraction estimation.
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Detects and removes contamination in genome assemblies via two disjoint workflows - foreign-sequence screening of a single-organism (eukaryote/isolate) assembly with NCBI FCS-GX (GenBank-submission-mandatory), FCS-adaptor, and BlobToolKit blob plots; and MAG/bin quality assessment with CheckM2 plus GUNC (chimerism) plus GTDB-Tk taxonomy, judged against MIMAG. Covers why CheckM2 alone is blind to disjoint-marker chimeras, the FCS-GX RAM wall, organelle/NUMT triage, strain heterogeneity, and the HGT-vs-contamination (tardigrade) trap. Use when screening an assembly for foreign contamination before GenBank submission, assessing MAG completeness/contamination/chimerism, deciding which contigs to remove, or distinguishing real HGT from contaminant contigs.
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Designs genomics experiments so technical nuisance variation (batch, lane, plate, flow cell, operator, reagent lot, processing day) is balanced against the biological variable of interest and therefore estimable rather than confounded, using constrained sample-to-batch assignment (designit, OSAT), the confounder/mediator/collider distinction, and the principle that no post-hoc correction recovers a fully confounded design. Covers detecting hidden batches with surrogate variable analysis, a decision table for downstream correction (ComBat-seq, RUVSeq, SVA) whose execution is deferred to differential-expression/batch-correction, and reproducibility metadata. Use when assigning samples to sequencing batches/lanes/plates, avoiding batch-condition confounding, deciding whether a design is salvageable by correction, choosing a correction method, or estimating the number of hidden batches. For the experimental unit, randomization, and blocking concepts see experimental-design/randomization-blocking.
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Reads, inspects, and writes Flow Cytometry Standard (FCS) files from conventional, spectral, and mass cytometry (CyTOF), and parses FlowJo/Cytobank/Diva workspaces. Covers FCS 2.0/3.0/3.1/3.2 internals ($PnE linear-vs-log, $DATATYPE, $SPILLOVER vs SPILL vs $COMP, $TIMESTEP), channel/parameter metadata, the silent linearize/truncate defaults, and R (flowCore, flowWorkspace, CytoML) plus Python (FlowKit, readfcs) readers. Use when loading flow or mass cytometry data, mapping detector channels to antibodies, extracting the event matrix, choosing a reader, or bridging FCS to the scanpy/AnnData ecosystem before preprocessing.
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Processes eDNA metabarcoding from raw paired-end reads to species tables, navigating ASV (DADA2, UNOISE3) vs OTU (swarm v2) decision (Callahan 2017 vs Schloss multi-copy-16S critique), marker/primer choice (Leray COI, MiFish 12S, 515F/806R 16S, ITS2) with primer-specific bias, OBITools3 v3 command-name break (obi stats plural; .tar.gz taxonomy), tag-jumping with dual-indexing (Schnell 2015; NovaSeq 10x MiSeq), decontam as screening-not-classifier (Davis 2018), read-counts-not-abundance critique (Lamb 2019), site-occupancy modeling (Ficetola 2015), Naive-Bayes calibration limits (Bokulich 2018), and eDNA decay (Strickler 2015). Use when going from raw eDNA FASTQ to species tables, picking marker + denoising pipeline, deciding whether read counts represent abundance, applying occupancy modeling, configuring OBITools3 v3, or interpreting decontam output. Not for clinical 16S microbiome (see microbiome/amplicon-processing).
日本語の概要は準備中です。原文の説明を表示しています。