「engineering」の検索結果

Systematic improvement of existing agents through performance analysis, prompt engineering, and continuous iteration.

日本語の概要は準備中です。原文の説明を表示しています。

Nominates and assesses CRISPR off-target sites genome-wide. Enumerates candidate sites by mismatch and bulge tolerance with Cas-OFFinder/CRISPRitz, ranks them with the published CFD score (SpCas9-only, relative ranker) or MIT/CRISTA/energy models, runs variant-aware screening against gnomAD/individual genomes (CRISPRme), and frames the empirical genome-wide discovery assays (GUIDE-seq, CIRCLE-seq, CHANGE-seq, DISCOVER-seq, Digenome-seq) and high-fidelity nuclease choice (HiFi Cas9, Sniper-Cas9, eSpCas9, SpCas9-HF1). Use when assessing guide RNA specificity, choosing among candidate guides, screening a therapeutic guide against population variation, or planning empirical off-target validation. Distinguishes predicted vs detected vs validated. On-target activity scoring and deaminase (Cas-independent) base/prime-editor off-targets are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。

Designs pegRNAs and nicking guides for prime editing (PE) -- choosing the nick/strand, tuning the primer-binding site (PBS) and reverse-transcription template (RTT) as a per-locus panel, selecting the PE system (PE2/PE3/PE3b/PE4/PE5/PEmax/PE7), adding MMR-evading and PAM-disrupting silent edits, appending epegRNA 3' motifs (tevopreQ1/mpknot), and ranking with PRIDICT/DeepPrime. Covers twinPE/PASTE for large insertions and the prime-vs-base-editing decision. Use when designing a scarless point mutation, small insertion/deletion, or any of the 12 base conversions without a double-strand break, when efficiency is low and MMR inhibition or pegRNA stabilization is needed, or when routing a large insertion to an integrase method. Generic guide scoring and base editing are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。

Designs donor/repair templates for precise CRISPR knock-ins -- choosing the format (ssODN, long-ssDNA/Easi-CRISPR, dsDNA/plasmid, AAV6), sizing homology arms, placing the cut within ~10 bp of the edit, and adding a mandatory codon-checked blocking (PAM/seed) mutation so the edited allele is not re-cut. Frames the HDR-vs-NHEJ-vs-MMEJ pathway competition, the MMEJ (PITCh) and homology-independent (HITI/HMEJ) alternatives for post-mitotic cells, ssODN strand/asymmetry choice, phosphorothioate end-protection, and ranked HDR enhancers. Use when designing a donor for a point mutation, epitope/fluorophore tag, allele replacement, or knock-in, or when HDR efficiency is low. Guide design and base/prime editing are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。

Designs cytosine (CBE, C-to-T) and adenine (ABE, A-to-G) base-editor guides by positioning the target base at the activity-peak of the editing window (protospacer positions ~5-7, PAM-distal numbering), minimizing bystander edits for product purity, reading dinucleotide context (APOBEC1 TC favored / GC disfavored), and selecting the editor variant (BE4max, ABEmax, ABE8e, YE1/SECURE, TadCBE, CGBE, SpG/SpRY-BE). Covers knockout by premature stop (CRISPR-STOP/iSTOP) and splice-site disruption, the three off-target classes (Cas-dependent, Cas-independent DNA, RNA), outcome prediction (BE-Hive/DeepBE), and the base-vs-prime-vs-HDR decision. Use when installing a transition mutation without a double-strand break, knocking out a gene without indels, or choosing CBE vs ABE. Generic guide scoring, prime editing, and HDR donors are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。

Designs and ranks guide RNAs (sgRNAs) for CRISPR-Cas9/Cas12a gene knockout by scanning a target for PAM sites (NGG SpCas9, NNGRRT SaCas9, TTTV Cas12a, NG SpCas9-NG, near-PAMless SpRY), enumerating candidate spacers, applying hard filters (Pol-III TTTT terminator, 5' G, GC), ranking on-target activity with the context-appropriate model (Rule Set 2/Azimuth for U6/lentiviral, CRISPRscan for T7/embryo, DeepHF for high-fidelity variants, DeepCpf1 for Cas12a), and predicting the indel/frameshift outcome (Bae out-of-frame score, inDelphi, FORECasT, Lindel). Use when selecting sgRNAs to knock out a gene, choosing a nuclease/PAM for a constrained locus, picking which exon to target, or shortlisting guides before an off-target check. Off-target specificity, base/prime editing, and HDR donors are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。

Build a differential-ready consensus peakset from per-replicate ATAC-seq peaks using iterative overlap removal, fixed-width re-centering, and majority-rule overlap. Use when generating a stable peak coordinate system for downstream differential accessibility, ML feature engineering, cross-sample comparison, or fixed-width peak counts; covers Corces 2018 iterative overlap (501 bp), DiffBind summit re-centering, and ENCODE consistency rules.

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CCSPlayerPawn_SnapViewAngles function in CS2 server binary using IDA Pro MCP. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate the non-virtual SnapViewAngles method, anchored on the "setang" console-command usage string and its non-teleport call branch. Emits a unique IDA-style byte signature and a func YAML. Trigger: CCSPlayerPawn_SnapViewAngles, SnapViewAngles

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CDecoyProjectile_Create factory in CS2 server binary using IDA Pro MCP. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate the decoy projectile spawn factory. It is a non-virtual, direct-call static factory found via the entity classname string "decoy_projectile" it references (excluding the RTTI class string "CDecoyProjectile"). Emits a unique IDA-style byte signature and a func YAML. Mirrors the find-CDecoyProjectile_Create.py preprocessor (xref_strings FULLMATCH:decoy_projectile). Trigger: CDecoyProjectile_Create, CDecoyProjectile::Create, decoy projectile Create

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CCSPlayerPawn_SnapEyeAngles function in CS2 server binary using IDA Pro MCP. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate the non-virtual SnapEyeAngles method. It is the one caller of the shared apply helper (CCSPlayerPawn_ApplyEyeAngleNetworkChange) that is NOT CCSPlayerPawn_SnapViewAngles. Emits a unique IDA-style byte signature and a func YAML. Mirrors the find-CCSPlayerPawn_SnapEyeAngles.py preprocessor (xref_funcs apply-helper, exclude_funcs SnapViewAngles). Trigger: CCSPlayerPawn_SnapEyeAngles, SnapEyeAngles

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CCSPlayer_ItemServices_DropActivePlayerWeapon virtual function in CS2 binary using IDA Pro MCP. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate the "drop the currently held weapon" vfunc by RTTI-walking the CCSPlayer_ItemServices vtable and confirming the candidate reads the player's active weapon and forwards it, with a computed toss velocity, into the weapon's own drop vfunc. Trigger: CCSPlayer_ItemServices_DropActivePlayerWeapon

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CCSPlayer_WeaponServices_EquipWeapon function and the CCSPlayer_WeaponServices_CanUse virtual function in CS2 binary using IDA Pro MCP. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate the weapon-equip helper and the "is this weapon usable right now" vfunc by RTTI-walking the CCSPlayer_WeaponServices vtable and confirming each candidate's decompiled body against the known CCSPlayer_WeaponServices_PickupItem anchor's callee shapes. Trigger: CCSPlayer_WeaponServices_EquipWeapon, CCSPlayer_WeaponServices_CanUse

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CCSPlayerController_Respawn virtual function in CS2 binary using IDA Pro MCP. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate the Respawn vfunc slot by RTTI-walking the CCSPlayerController vtable and confirming the candidate's decompiled body performs a full controller-state reset (networked flags, per-round fields, comeback/damage-info bookkeeping) rather than any other lifecycle vfunc. Trigger: CCSPlayerController_Respawn

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CCSNavArea_IsValidNavMesh function in CS2 server binary using IDA Pro MCP. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate the nav-mesh validity predicate. It is a tiny non-virtual-looking helper that returns `g_pNavMesh != nullptr` (15 bytes), referenced only from a vtable slot, with no debug string of its own. Emits a unique IDA-style byte signature and a func YAML. Mirrors the find-CCSNavArea_IsValidNavMesh.py preprocessor (xref_gvs g_pNavMesh intersected with the tail signature `48 83 38 ? 0F 95 C0 C3`). Trigger: CCSNavArea_IsValidNavMesh, IsValidNavMesh, g_pNavMesh predicate

日本語の概要は準備中です。原文の説明を表示しています。

Find and patch the water jump velocity inside CCSPlayer_MovementServices_CheckJumpButton in CS2 binary using IDA Pro MCP. This patch changes the water jump velocity from 100.0f to 145.0f by modifying the immediate operand of a mov instruction. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate and patch the water jump height value. Trigger: water jump patch, CheckJumpButton water velocity, jump height patch, water jump 145

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the velocity clamping branch inside CCSPlayer_MovementServices_FullWalkMove in CS2 binary using IDA Pro MCP, then generate a patch signature to disable it. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate and patch the speed-clamp if-branch that caps player velocity to maxspeed inside FullWalkMove. Trigger: FullWalkMove speed clamp, velocity clamping patch, FullWalkMove SpeedClamp, disable maxspeed clamp

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CCSGameRules_PostCleanUp function in CS2 server binary using IDA Pro MCP. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate the post-round entity-cleanup routine. It is non-virtual and has no unique string of its own, so it is found via the CCSGameRules_ResetRound predecessor: PostCleanUp is the entity-cleanup call ResetRound makes. Emits a unique IDA-style byte signature and a func YAML. Mirrors the find-CCSGameRules_PostCleanUp.py preprocessor (LLM_DECOMPILE CCSGameRules_ResetRound). Trigger: CCSGameRules_PostCleanUp, PostCleanUp, CGameRules::PostCleanUp

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CCSGameRules_ResetRound function in CS2 server binary using IDA Pro MCP. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate the round-reset routine. It is the only function that references the "GMR_ResetRound\n" debug log string, and is the predecessor anchor for find-CCSGameRules_PostCleanUp (which it calls). Emits a unique IDA-style byte signature and a func YAML. Mirrors the find-CCSGameRules_ResetRound.py preprocessor (xref_strings "GMR_ResetRound\n"). Trigger: CCSGameRules_ResetRound, ResetRound, GMR_ResetRound

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CCSPlayer_ItemServices_GiveNamedItem virtual function, the CCSPlayer_WeaponServices_Weapon_GetSlot helper, and the CBasePlayerPawn_RemovePlayerItem helper in CS2 binary using IDA Pro MCP. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate GiveNamedItem by RTTI-walking the CCSPlayer_ItemServices vtable, then to locate the other two by decompiling the already-known CCSPlayer_ItemServices_GiveDefaultItems anchor and matching its callees' argument shapes and inventory-slot bookkeeping semantics. Trigger: CCSPlayer_ItemServices_GiveNamedItem, CCSPlayer_WeaponServices_Weapon_GetSlot, CBasePlayerPawn_RemovePlayerItem

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CCSGameRules::TerminateRound function in the CS2 server binary using IDA Pro MCP. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate the round-end-processing function by finding the code that references both the bare "TerminateRound" debug tag string and the "TerminateRound: unknown round end ID %i\n" warning-format string — both are referenced from the same function. Trigger: CCSGameRules_TerminateRound

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CBaseTrigger::StartTouch and CBaseTrigger::EndTouch overridden virtual functions in the CS2 server binary using IDA Pro MCP. Use this skill when reverse engineering server.dll or libserver.so to locate the trigger-volume touch-list add/remove handlers by RTTI-walking CBaseTrigger's and CBaseEntity's primary vtables and diffing them slot-by-slot to find CBaseTrigger's overrides, then identifying which two overridden slots implement "add pOther to the touching-entities list" (StartTouch) versus "remove pOther from the touching-entities list" (EndTouch). Trigger: CBaseTrigger_StartTouch, CBaseTrigger_EndTouch

日本語の概要は準備中です。原文の説明を表示しています。

Find and patch the g_pNavMesh null-check inside CCSBotManager_AddBot in CS2 binary using IDA Pro MCP. This patch removes the navigation mesh requirement so bots can be added even without a nav mesh loaded. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate and patch the nav mesh check in AddBot. Trigger: bot nav ignore, AddBot nav mesh patch, bot without nav, CCSBotManager nav ignore

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CBasePlayerPawn::CommitSuicide virtual function's vtable slot in the CS2 server binary using IDA Pro MCP. Use this skill when reverse engineering server.dll or libserver.so to locate the self-inflicted-death handler (used by the "kill"/bot_kill console commands and related gameplay code) by RTTI-walking CBasePlayerPawn's primary vtable and reading its known slot index directly, then confirming the body matches CommitSuicide(bool bExplode, bool bForce) semantics. Trigger: CBasePlayerPawn_CommitSuicide

日本語の概要は準備中です。原文の説明を表示しています。

Find and identify the CCSGameRules__sm_mapGcBanInformation global variable in CS2 binary using IDA Pro MCP. Use this skill when reverse engineering CS2 server.dll or libserver.so to locate the GC ban information map structure by decompiling CCSPlayerController_ResourceDataThink's sub-function (Linux) or searching for the "Notification about user penalty" string (Windows).

日本語の概要は準備中です。原文の説明を表示しています。