221 件 ・ 関連度順
概要と使いどころ
Builds automated threat intelligence feed integration pipelines connecting STIX/TAXII feeds, open-source threat intel, and commercial TI platforms into SIEM and security tools for real-time IOC matching and alerting. Use when SOC teams need to operationalize threat intelligence by automating feed ingestion, normalization, scoring, and distribution to detection systems.
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Correlate events across multiple log files from different sources, systems, or time periods. Build unified timelines, identify causal relationships between events, and highlight anomalies that span multiple data sources. Supports timezone normalization and gap detection. Use when analyzing logs from multiple systems (frontend, backend, database, infrastructure), investigating distributed system failures, or building event timelines across services. Triggers: "correlate these logs", "build timeline from multiple logs", "cross-system analysis", "find related events across logs", "multi-source log analysis", "distributed trace analysis"
日本語の概要は準備中です。原文の説明を表示しています。
Knowledge base from the NASA Cost Estimating Handbook (CEH) v4.0. Use for NASA's 12-step, three-part cost estimating process (Project Definition, Cost Methodology, Cost Estimate); the WBS, CADRe, and ONCE data backbone; estimating methodologies (analogy, parametric/CER, engineering build-up, EVM, Delphi); ground rules and assumptions, data normalization and inflation (BY/CY/RY); the point-estimate-to-S-curve cost-risk chain and Unallocated Future Expense (UFE) reserves; Joint Cost and Schedule Confidence Level (JCL) analysis via probabilistic cost-loaded schedules; decision-support analyses (sensitivity, trade studies, make/lease-vs-buy, affordability, CAIV); and economic analysis (discounting, NPV, OMB Circular A-94). The cost-estimating complement to NASA's SE and risk packs. Does NOT reproduce the handbook's tool-specific procedures, worked examples, or appendix-depth methods (it names the appendices), nor cover full DoD/GAO cost guides, schedule construction, or formal risk management beyond the cost interface.
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Enforces error handling standards: silent error prohibition, custom error class hierarchy (AppError base with category never-fallback / transient / permanent, cause preservation, ValidationError / UnauthorizedError / UpstreamError etc. — canonical definitions in PATTERNS.md), external-boundary normalization (normalizeExternalError), Result pattern (Result.ok/Result.fail), proper try-catch with error type checking, structured error logging with a single Logger contract, fallback prohibition categories (isNeverFallback), and HTTP status code mapping. Use when implementing error handling, reviewing catch blocks, or designing error responses.
日本語の概要は準備中です。原文の説明を表示しています。
Design database schemas with normalization, relationships, and constraints. Use when creating new database schemas, designing tables, or planning data models for any database paradigm.
日本語の概要は準備中です。原文の説明を表示しています。
MSBuild property definition patterns: conditional defaults, composition/concatenation, path normalization, trailing slash handling, TFM detection helpers, and property evaluation order. Only activate in MSBuild/.NET build context. USE FOR: diagnosing and fixing MSBuild property definition issues in .props or .csproj files, reviewing and fixing shared property configuration anti-patterns, fixing DefineConstants or NoWarn being overwritten instead of appended, fixing unconditional property assignments that prevent project-level overrides, fixing unquoted conditions that fail when properties are empty, fixing hardcoded paths that break cross-platform builds, setting property defaults that can be overridden, understanding property evaluation order and last-write-wins semantics. DO NOT USE FOR: props vs targets placement (use directory-build-organization), item operations (use item-management), target structure (use target-authoring), general anti-patterns (use msbuild-antipatterns), non-MSBuild build systems.
日本語の概要は準備中です。原文の説明を表示しています。
Scanpy is a scalable Python toolkit for analyzing single-cell RNA-seq data, built on AnnData. Apply this skill for complete single-cell workflows including quality control, normalization, dimensionality reduction, clustering, marker gene identification, visualization, and trajectory analysis.
日本語の概要は準備中です。原文の説明を表示しています。
Builds automated threat intelligence feed integration pipelines connecting STIX/TAXII feeds, open-source threat intel, and commercial TI platforms into SIEM and security tools for real-time IOC matching and alerting. Use when SOC teams need to operationalize threat intelligence by automating feed ingestion, normalization, scoring, and distribution to detection systems.
日本語の概要は準備中です。原文の説明を表示しています。
Design database schemas — tables, relationships, indexes, constraints, and ORM setup. Covers relational design, normalization, and common patterns.
日本語の概要は準備中です。原文の説明を表示しています。
Build genome-browser-style multi-track figures with pyGenomeTracks (config-driven), Gviz (R), and IGV batch screenshotting. Covers BigWig coverage tracks, BED/peak overlays, gene-model rendering, Hi-C matrix tracks, BedPE link arcs, spike-in-aware normalization, and the bamCoverage --normalizeUsing trap. Use when producing publication figures of genomic loci with stacked aligned tracks (coverage, peaks, genes, interactions) for ChIP-seq, ATAC-seq, RNA-seq, Hi-C, or generic locus visualization.
日本語の概要は準備中です。原文の説明を表示しています。
End-to-end proteomics workflow from MaxQuant output to differential protein abundance. Orchestrates data import, normalization, imputation, and statistical testing with limma (default) or MSstats for complex feature-level designs. Use when processing mass spectrometry proteomics.
日本語の概要は準備中です。原文の説明を表示しています。
End-to-end single-cell RNA-seq workflow from 10X Genomics data to annotated cell types. Covers QC, normalization, clustering, marker detection, and cell type annotation. Use when analyzing single-cell RNA-seq data.
日本語の概要は準備中です。原文の説明を表示しています。
Orchestrates the end-to-end germline short-variant pipeline from FASTQ to a filtered, normalized, benchmarked VCF, chaining QC/trim, BWA-MEM2 alignment, duplicate marking, optional BQSR, calling (bcftools/GATK HaplotypeCaller/DeepVariant/DRAGEN), normalization, site+genotype filtering, annotation, and hap.py/vcfeval benchmarking. Use when deciding the pipeline-wide reference-genome commitment (GRCh38 analysis set vs T2T, ALT/decoy handling), sequencing the steps in the defensible order (normalize BEFORE annotate, filter site- then genotype-level), choosing the calling engine and single-sample vs cohort joint-calling, picking a filtering strategy by cohort size, or benchmarking stratified within GIAB confident regions. Hands off mechanism to the variant-calling and read-alignment component skills; not a re-teach of any single step.
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Orchestrates the untargeted LC-MS metabolomics pipeline end-to-end (xcms 4.x feature extraction, QC/drift/normalization, confidence-stratified annotation, permutation-validated statistics, background-aware pathway mapping), naming what each stage decides and where it silently fails. Use when running a full LC-MS metabolomics study from raw mzML to enriched pathways and needing the honest handoffs between stages. Each stage defers to its component skill for parameters and traps; for stable-isotope flux (a separate branch, not this untargeted flow) see metabolomics/isotope-tracing.
日本語の概要は準備中です。原文の説明を表示しています。
Programmatic untargeted LC-MS feature extraction in R with the modern xcms 4.x MsExperiment/XcmsExperiment API, taking raw mzML to a feature table via CentWave peak detection, retention-time alignment, peak-density correspondence, gap-filling, CAMERA redundancy collapse, and built-in QC feature filtering. Use when converting centroided LC-MS runs into a features-by-samples matrix and deciding centWave/grouping/alignment parameters. For drift correction and QC/CV filtering execution see metabolomics/normalization-qc; for metabolite identification see metabolomics/metabolite-annotation; for the MS-DIAL GUI alternative with MS2Dec deconvolution see metabolomics/msdial-preprocessing; for downstream statistics see metabolomics/statistical-analysis.
日本語の概要は準備中です。原文の説明を表示しています。
Standardizes molecular structures using ChEMBL chembl_structure_pipeline and RDKit rdMolStandardize covering sanitization, salt/solvent stripping, neutralization, tautomer canonicalization, stereochemistry standardization, mixture handling, and isotope normalization. Explicitly compares ChEMBL pipeline, canSARchem, and PubChem standardization choices. Use when preparing libraries for QSAR training, joining datasets across sources, deduplicating compound collections, or building canonical compound registries.
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Runs the MS-DIAL preprocessing workflow (peak picking, MS2Dec spectral deconvolution, alignment, gap-filling) and imports the alignment-result table into R or Python with honest filtering. Use when preprocessing LC-MS DDA/DIA (SWATH) raw data with MS-DIAL, deciding MS-DIAL vs XCMS, configuring the MsdialConsoleApp console run, or parsing an MS-DIAL export into a clean feature matrix. For programmatic R peak detection and the feature-table-as-artifact framing see metabolomics/xcms-preprocessing; for lipid annotation mode see metabolomics/lipidomics; for MSI-level confidence honesty see metabolomics/metabolite-annotation; for drift correction and QC see metabolomics/normalization-qc.
日本語の概要は準備中です。原文の説明を表示しています。
How to identify and test for account takeover vulnerabilities in web applications. Use this skill whenever the user mentions account takeover, authentication bypass, password reset attacks, email verification bypass, session hijacking, or any technique to compromise user accounts. This includes testing authorization issues, unicode normalization attacks, reset token reuse, CORS/CSRF/XSS exploitation, cookie manipulation, and OAuth vulnerabilities.
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Generates, normalizes, and converts bedGraph signal tracks (4-column chrom/start/end/value, 0-based half-open) with bedtools genomecov, deepTools bamCoverage/bamCompare/bigwigCompare, bedtools unionbedg, and UCSC bedGraphToBigWig. Covers why a raw coverage bedGraph is not comparable across samples until normalized, the CPM/RPKM/BPM/RPGC normalization menu and the conserved-total assumption that makes them wrong under a global perturbation, the strict sorted-non-overlapping-chrom.sizes bedGraphToBigWig contract that silently corrupts a bigWig, effective-genome-size selection, and bin-size aliasing. Use when building or normalizing a coverage/signal track from a BAM, comparing tracks across samples or conditions, converting bedGraph to a browser-ready bigWig, or diagnosing a track that looks plausible but reports wrong heights.
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Renders Hi-C contact matrices honestly and reproducibly with matplotlib, cooltools, HiCExplorer, pyGenomeTracks, FAN-C, CoolBox, and plotgardener. Covers the raw/ICE-balanced/observed-over-expected transform choice, LogNorm vs symmetric-diverging colormaps with vmax/percentile clipping, resolution-to-feature matching (compartments 100-500kb, TADs 10-40kb, loops 5-10kb), square vs rotated-triangle track-stacking, NaN/white-stripe handling, virtual 4C, APA/saddle/on-diagonal pileups, two-condition side-by-side and log2-ratio maps, and interactive (HiGlass) vs scripted-static publication figures. Use when plotting a contact matrix, choosing a normalization or color scale, building a multi-track Hi-C figure, making a virtual 4C profile, piling up loops/boundaries, or comparing two conditions.
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Compares Hi-C contact maps between conditions across the right scale -- differential bin-pair contacts (multiHiCcompare, diffHic), differential A/B compartments (dcHiC), differential TAD boundaries (delta insulation), and differential loops (diffloop, DiffHiChIP) -- with distance-stratified between-sample normalization, replicate-aware NB-GLM FDR, HiCRep SCC reproducibility gating, and CNV correction for cancer/aneuploid samples. Use when comparing Hi-C between treatment and control, finding differential contacts/compartments/boundaries/loops, normalizing two maps of unequal depth, choosing a replicate-aware test, gating replicates with SCC, or correcting copy-number artifacts before a tumor-vs-normal comparison.
日本語の概要は準備中です。原文の説明を表示しています。
Performs differential expression on bulk RNA-seq count data with edgeR's negative-binomial GLM and quasi-likelihood F-test framework. Covers DGEList construction, filterByExpr, TMM/TMMwsp normalization, robust dispersion estimation, glmQLFit/glmQLFTest, TREAT for magnitude-bounded hypotheses, contrasts via no-intercept designs, voom and voomWithQualityWeights for heterogeneous samples, and the edgeR v4 bias-corrected APL changes. Use when running bulk DE with edgeR, choosing edgeR over DESeq2 (small n, transcript DE via catchSalmon, large samples), needing TREAT for a fold-change-threshold hypothesis, troubleshooting v3-to-v4 reproducibility, building paired or interaction designs, or handling library-quality heterogeneity.
日本語の概要は準備中です。原文の説明を表示しています。
Build genome-browser-style multi-track figures with pyGenomeTracks (config-driven), Gviz (R), and IGV batch screenshotting. Covers BigWig coverage tracks, BED/peak overlays, gene-model rendering, Hi-C matrix tracks, BedPE link arcs, spike-in-aware normalization, and the bamCoverage --normalizeUsing trap. Use when producing publication figures of genomic loci with stacked aligned tracks (coverage, peaks, genes, interactions) for ChIP-seq, ATAC-seq, RNA-seq, Hi-C, or generic locus visualization.
日本語の概要は準備中です。原文の説明を表示しています。
Quality control for pooled CRISPR screens covering library representation, Gini index, log-skew, replicate Pearson and Spearman concordance, essentialome precision-recall AUC against CEGv2 (Hart 2017), Cas9 cut-toxicity diagnostics, copy-number amplicon detection (Aguirre 2016 / Munoz 2016), bottleneck propagation through plasmid pool, infection, selection, and endpoint stages, MOI verification, and DepMap-style screen-quality scoring. Use when assessing screen quality before hit calling, deciding whether to repeat or rescue a screen, diagnosing low-confidence hits, choosing between MAGeCK / BAGEL2 / Chronos based on quality grade, picking a normalization strategy from QC signatures, or evaluating whether an in-vivo screen retained adequate library complexity.
日本語の概要は準備中です。原文の説明を表示しています。