「sse」の検索結果

Constructs and validates polygenic risk scores using LDpred2-auto, SBayesRC, MegaPRS, PRS-CS, PROSPER, MUSSEL, BridgePRS, JointPRS, PRSmix, or PGS Catalog Calculator with ancestry-aware reference panels (HapMap3, UKB-LD), Pejaver-style calibration, and PRS-RS reporting standards. Use when computing PRS for cohorts, applying Whiffin-style absolute-risk transformation, assessing cross-ancestry portability (Martin 2017 / Ding 2023 continuous ancestry), or auditing PRS manuscripts against the 22-item PRS-RS reviewer checklist.

日本語の概要は準備中です。原文の説明を表示しています。

Align preprocessed CLIP-seq reads (eCLIP, iCLIP, iCLIP2, PAR-CLIP) to genome with STAR or bowtie2 using crosslink-preserving parameters, choosing between unique-mapper-only and multi-mapper-aware alignment for repeat-binding RBPs, deciding STAR vs HISAT2 memory trade-offs, and applying ENCODE-compatible filters. Use when turning preprocessed CLIP FASTQ into a deduplicated, MAPQ-filtered BAM ready for peak calling or crosslink-site detection.

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Assesses ChIP-seq quality across antibody specificity, fragmentation, enrichment, replicate concordance, and library complexity. Computes FRiP, NSC/RSC (phantompeakqualtools), library complexity (NRF/PBC1/PBC2), deepTools plotFingerprint (JS distance, AUC, synthetic JS), ChIPQC, IDR with ENCODE Nself/Nt rules, and detects hyper-ChIPable artifacts. Use when validating an antibody, diagnosing failed peak calls, deciding whether to proceed with downstream analysis, grading against ENCODE thresholds, or auditing replicate concordance.

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Assesses RNA-seq data quality specifically for alternative splicing analysis. QC layers include experimental design audit (library prep, read length, depth, replicates), STAR 2-pass cohort-style alignment, junction saturation curves and discovery plateau detection, novel-vs-known junction ratio diagnostics, junction-overhang distribution, splice-site strength scoring (MaxEntScan intrinsic + SpliceAI context-aware), strandedness verification, GENCODE basic vs comprehensive choice, and rRNA contamination screening. Splicing analysis is more demanding than DGE on read length, depth, library prep, alignment strategy, and annotation choice — failures silently bias PSI estimates and inflate novel-junction false positives. Use when evaluating data suitability for splicing analysis, troubleshooting low event detection, or designing sequencing experiments where AS is a primary endpoint.

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Quantifies alternative splicing as PSI (percent spliced in) from RNA-seq using rMATS-turbo (BAM-based event), SUPPA2 (TPM-based event), MAJIQ V3 (LSV-based Bayesian), leafcutter (annotation-free intron clusters), VAST-TOOLS (cross-species with microexon support), Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage), or IRFinder-S (intron retention coverage-aware). Distinguishes the five canonical event classes (SE, A5SS, A3SS, MXE, RI), special classes (microexons, exitrons, AFE/ALE), intron retention subtypes (canonical RI vs detained introns), and applies effective-length normalization. Use when measuring splice-site usage or isoform inclusion ratios from short-read RNA-seq.

日本語の概要は準備中です。原文の説明を表示しています。

ATAC-seq library quality control -- TSS enrichment, FRiP, fragment-size periodicity, library complexity (NRF/PBC1/PBC2), mitochondrial fraction, and ENCODE 4 thresholds. Use when assessing whether an ATAC-seq library passes ENCODE acceptance criteria, diagnosing transposition artefacts, comparing Omni-ATAC vs standard prep quality, or selecting which replicates to drop before peak calling.

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Aumenta resolução e qualidade de imagem via Freepik/Magnific, ou orienta o processo manual quando não há chave. Use quando o usuário disser "upscale", "melhorar a resolução", "a imagem está pixelada", "Magnific", "Freepik", "aumentar a imagem", "imagem pequena demais". Gasta crédito — exige confirmação.

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Organiza, compara e seleciona entre as variações geradas, e publica a grade de comparação como Artifact para aprovação do time. Use quando o usuário disser "qual dessas", "comparar as variações", "escolher a melhor", "mostrar as opções", "montar grade de aprovação", ou após qualquer lote de geração. Selecionar é trabalho, não detalhe.

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Escreve texto alternativo e descrição de imagem para peças de rede social. Use quando o usuário disser "alt text", "texto alternativo", "descrição da imagem", "acessibilidade da peça", "descrever para leitor de tela", ou automaticamente antes de qualquer publicação. Obrigatório em todas as peças — em carrossel, um por slide.

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Segundo cérebro persistente em Markdown — LLM Wiki no padrão Karpathy mantida como bundle Open Knowledge Format v0.2, com fontes imutáveis em raw/ e conceitos citados em wiki/. Use quando o usuário disser "segundo cérebro", "second brain", "adiciona isso ao meu brain", "salva na minha wiki", "ingere esse artigo/PDF/link", "o que minha wiki diz sobre", "o que meu brain sabe sobre", "consulta meu conhecimento", "lint da wiki", ou pedir para acumular conhecimento de fontes de forma durável. NÃO use para notas do Obsidian (pwdev-obsidian), planejamento de features (pwdev-feat) nem PRDs (pwdev-prd).

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Apply continuous improvement principles to iteratively enhance processes, code quality, team practices, and system performance through small, incremental changes. Use when improving development processes, optimizing workflows, reducing waste, enhancing code quality over time, implementing retrospective learnings, or fostering a culture of continuous improvement.

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Architect scalable backend services using layered architecture, dependency injection, middleware patterns, service classes, and separation of concerns. Use when building API services, implementing business logic layers, creating service classes, setting up middleware chains, implementing dependency injection, designing controller-service-repository patterns, handling cross-cutting concerns, creating domain models, implementing CQRS patterns, or establishing backend architecture standards.

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When the user wants help with revenue operations, lead lifecycle management, or marketing-to-sales handoff processes. Also use when the user mentions 'RevOps,' 'revenue operations,' 'lead scoring,' 'lead routing,' 'MQL,' 'SQL,' 'pipeline stages,' 'deal desk,' 'CRM automation,' 'marketing-to-sales handoff,' 'data hygiene,' 'leads aren't getting to sales,' 'pipeline management,' 'lead qualification,' or 'when should marketing hand off to sales.' Use this for anything involving the systems and processes that connect marketing to revenue. For cold outreach emails, see cold-email. For email drip campaigns, see email-sequence. For pricing decisions, see pricing-strategy.

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Resize, crop, or export any image or video into platform-ready social media assets using Adobe Creative Cloud tools. Use this skill when a user wants to prepare a photo, image, or video for one or more social platforms — Instagram, TikTok, LinkedIn, Facebook, YouTube, Snapchat, Pinterest, Threads, or X/Twitter. Triggers on: "prepare my image for Instagram", "resize for TikTok", "get this ready to post", "make versions for all platforms", "social media sizes", "crop for stories", "export for LinkedIn", "resize my video for social", "make social media assets", or any request to adapt a photo or video for specific platforms. Handles subject-aware cropping, AI canvas expansion, test previews before full runs, and same-ratio video resizing.

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Initialize an Adobe App Builder project end-to-end without Developer Console UI clicks. Creates the Console project and workspace, subscribes APIs (including those needing a product profile), maps user intent to the right template, runs non-interactive `aio app init`, and guides post-init customization. Use whenever the user mentions creating an App Builder app, scaffolding a project, `aio app init`, setting up an Experience Cloud extension, adding actions or web assets, creating a Console project or workspace, adding APIs, or bootstrapping App Builder — even if they don't say "App Builder". Also for SPA templates, AEM extensions, API Mesh, Asset Compute workers, and MCP server projects. Also handles debugging init failures — template not found, `aio app init` hangs or times out, Node version mismatches, npm install failures, post-init build errors, `aio login` issues, `aio app run` showing nothing, or `aio console project create` / `workspace create` / `workspace api add` errors.

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UI 預覽評審 SKILL — 截取 Cocos Editor Preview 畫面，與 proof draft 提供的參考圖逐 zone 比對，輸出視覺信心分數與 PASS/FAIL 評審報告，並回寫 runtime-verdict。USE FOR: ui-asset-qc 通過後，需要人工確認 + 自動評審的最終視覺驗收。DO NOT USE FOR: Runtime 崩潰（用 cocos-log-reader）、Browser QA 截圖（用 cocos-preview-qa）、資產規格問題（用 ui-asset-qc）。

日本語の概要は準備中です。原文の説明を表示しています。

UI 資產品質驗證 SKILL — 執行 validate-visual-assets.js、validate-ui-specs.js 與必要的 snapshot regression，對 skin family 資產、screen package、proof draft / family-map 進行全面驗證，並回寫 generated-review。USE FOR: 任何 UI 資產生成後、在 ui-preview-judge 前必須先通過的 QA 關卡。DO NOT USE FOR: debug runtime 崩潰（用 cocos-log-reader）、Layout 佈局調整（用 ui-vibe-pipeline）。

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UI 資產切件與候選收斂 SKILL — 從使用者提供的整頁 UI、2K 母圖、panel sheet 或單張素材，自動執行全切、auto-classify、auto-pick、temp/selected 分流、背景裁切、透明化與 post-process。USE FOR: 切出 panel / cap / badge / glyph / noise 候選、把未挑選切片集中到 temp 可一次清掃、把高信心候選升到 selected、再接 trim-png-by-background.js / postprocess-ui-asset.js。DO NOT USE FOR: runtime 畫面驗收（用 ui-runtime-verify）、純 icon 規格設計（用 UI-icon workflow）、單純修改 layout/skin JSON。

日本語の概要は準備中です。原文の説明を表示しています。

Orchestrator for the eval-output skill suite — evaluate LLM and agent outputs for quality, accuracy, helpfulness, and safety using structured rubrics and LLM-as-judge techniques. Load when the user says "evaluate this output", "score this response", "run an eval", "LLM as judge", "evaluate agent output", "how good is this response", "rate this answer", "eval this", or provides an LLM output that should be assessed for quality. Single entry point for all output evaluation workflows.

日本語の概要は準備中です。原文の説明を表示しています。

Automate Coassemble tasks via Rube MCP (Composio). Always search tools first for current schemas.

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Transfers gene annotations between genome assemblies via coordinate liftover (UCSC liftOver, CrossMap for same-species version updates) or feature/sequence projection (Liftoff for same/close species, miniprot for protein-level cross-species, TOGA/GeMoMa/CAT for distant clades). Covers the coordinate-vs-projection decision by divergence, why a successful lift is not biological confirmation, reference bias, the silent-dropping of unmapped features, build/PAR/MHC/inversion hazards, and transfer-vs-de-novo validation. Use when annotating a new assembly of a species with an existing reference, harmonizing coordinates across builds, or mapping annotations across related species.

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Assesses genetic health of populations for conservation with Ne estimation across time horizons (LDNe NeEstimator V2 option-file API + SNeP physical-linkage correction; recent trajectory via GONE/GONE2; deep history via Stairway Plot 2 / dadi / fastsimcoal2 / PSMC), F-statistics, runs of homozygosity binned by length class to date inbreeding, genetic-load decomposition (Bertorelle 2022 realized vs masked), the modern 100/1000 Ne rule (Frankham 2014), Ne/Nc 2-6 orders of magnitude in marine fish (Hauser & Carvalho 2008), tree-sequence forward simulations (SLiM 4 + pyslim + tskit), and the Sukumaran-Knowles caveat against MSC methods for management-unit definition. Use when estimating Ne by time horizon, detecting inbreeding via F_ROH, decomposing genetic load, justifying conservation thresholds, distinguishing ESU/MU/DPS, configuring NeEstimator V2, or correcting LDNe physical linkage.

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Corrects the gene-independent copy-number artifact in CRISPR-Cas9 screens (Aguirre 2016 / Munoz 2016 Cancer Discov) where amplified loci appear essential from DNA-damage burden of simultaneous cuts. Covers the p53-dependent G2-arrest mechanism, CRISPRcleanR (Iorio 2018) unsupervised pre-hoc correction, CERES (Meyers 2017) joint CN + gene-effect model, Chronos (Dempster 2021) DepMap-standard population-dynamics + CN model with lowest residual bias, the decision tree by data availability, the Spearman LFC-vs-CN diagnostic, focal-amplification examples (ERBB2 in HER2+, MYC in colorectal, FGFR1 in head and neck), and CRISPRi/a alternatives that bypass the artifact. Use when screening cancer cell lines, diagnosing essentiality at amplified loci, choosing CRISPRcleanR / CERES / Chronos, deciding whether CN correction is needed before MAGeCK / BAGEL2 / drugZ, or switching from Cas9 to CRISPRi.

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Quality control for pooled CRISPR screens covering library representation, Gini index, log-skew, replicate Pearson and Spearman concordance, essentialome precision-recall AUC against CEGv2 (Hart 2017), Cas9 cut-toxicity diagnostics, copy-number amplicon detection (Aguirre 2016 / Munoz 2016), bottleneck propagation through plasmid pool, infection, selection, and endpoint stages, MOI verification, and DepMap-style screen-quality scoring. Use when assessing screen quality before hit calling, deciding whether to repeat or rescue a screen, diagnosing low-confidence hits, choosing between MAGeCK / BAGEL2 / Chronos based on quality grade, picking a normalization strategy from QC signatures, or evaluating whether an in-vivo screen retained adequate library complexity.

日本語の概要は準備中です。原文の説明を表示しています。