「saturation」の検索結果

Use when planning and synthesizing product/user research as a method-and-repository discipline — selecting the right method for the goal (generative interviews vs usability test vs concept test vs validation), computing method-based saturation/sample size with an explicit confidence level, or synthesizing coded observations into insights while flagging single-source anecdotes. Never fabricates user insight; an insight requires recurrence across independent participants. Distinct from product-team/ux-researcher-designer (persona/journey artifacts), product-discovery (discovery-sprint planning), and experiment-designer (live A/B) — this is the research-ops method + insight-repository layer.

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Maps whether a biomedical research topic, subtopic, or study angle is truly saturated, superficially crowded, strategically occupied, or still open for differentiated entry. Use this skill when a user wants to know whether a hot medical research direction is already overworked, whether meaningful whitespace remains, whether major groups have already occupied the obvious claims, and whether the timing window is still open. Always distinguish popularity from true saturation, and distinguish cosmetic novelty from meaningful differentiating entry.

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Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for visualisation.

日本語の概要は準備中です。原文の説明を表示しています。

Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for visualisation.

日本語の概要は準備中です。原文の説明を表示しています。

Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq c_curve / lc_extrap, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT — see Tips), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for downstream cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for downstream visualisation.

日本語の概要は準備中です。原文の説明を表示しています。

Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq c_curve / lc_extrap, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT — see Tips), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for downstream cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for downstream visualisation.

日本語の概要は準備中です。原文の説明を表示しています。

Runs maximum-saturation research with a cooperating team, claim-graph gating, and a cited, QA'd deliverable. Use when the user explicitly asks for research or a deep investigation, including any 'ulw' research wording.

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Use when audio already placed in a HyperFrames composition needs to be mixed: fade-in/fade-out, crossfade, track gain or volume, volume automation, ducking, a music bed that fights a voiceover (voiceover carve), effects on a track (EQ, compressor, limiter, gate, saturation, delay, reverb, chorus, phaser, bitcrush), automation envelopes drawn on a track's volume or any effect parameter, or one submix bus carrying a chain, a fader and an automation clock for several tracks at once (`<hf-audio-group>`). Don't use for sourcing or generating audio — finding BGM, SFX, or making a voiceover is `/media-use`. Don't use for clip timing or track layout, which is `/hyperframes-core`.

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Solves seawater carbonate chemistry with PyCO2SYS for chemical oceanography, ocean acidification, and marine carbon-cycle research. Use for paired total alkalinity, dissolved inorganic carbon, pH, or seawater pCO2/fCO2 measurements; carbonate speciation; aragonite and calcite saturation; Revelle factors; lab-to-in-situ temperature and pressure corrections; and measurement uncertainty propagation. Applies to carbonate-system calculations, not general aqueous speciation or air-sea gas-flux estimation.

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Deep-research a topic end to end and produce a permanent, reusable knowledge asset: archive every primary source verbatim (gated by the user's privacy/retention posture), write one 1:1 summary per source, then synthesize a single self-contained compendium page. Depth is a dial (base synthesis → grounded primaries → books + counter-canon → saturation), each level an idempotent superset of the one below. Distinct from data-research (structured trackers) and perplexity-research (web deltas): this produces prose knowledge synthesis backed by an archived source corpus.

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Diagnose GKE Cloud TPU training throughput drops and step-time regressions (15%+ TPU duty-cycle drop) using ML Diagnostics Workload Monitoring (`gcloud alpha mldiagnostics monitored-events` / `hypercomputecluster.googleapis.com/v1alpha`) and 1-minute Cloud Monitoring system metrics (`kubernetes.io/node/accelerator/*`). Distinguishes hardware and network fabric throttling from workload resource bottlenecks (HBM capacity, host memory, or host CPU saturation). Use when TPU training throughput or duty cycle drops without crashing pods, when `PERFORMANCE_DEGRADATION` monitored events fire, or when triaging slow multi-slice training steps. Don't use for complete multi-slice XLA execution stalls with `HANG_DETECTED` logs (use gke-ai-troubleshooting-tpu-mxla-hang) or pod eviction/interruption restarts (use gke-ai-troubleshooting-jobset-interruption).

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Configures alerting policies in Terraform for Google Kubernetes Engine (GKE) clusters, workloads, and services using PromQL and Google Cloud Managed Service for Prometheus. Use when writing, analyzing, validating, or deploying Terraform alerting policies to monitor GKE service latency, traffic, error rates using Multi-Window Multi-Burn-Rate SLO alerts, memory saturation, and cluster health such as CrashLoopBackOff and Node NotReady conditions. Don't use for non-GKE compute runtimes such as standalone Compute Engine VMs or standalone Cloud Run services without GKE.

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Provides data-retrieval best practices, tool selection guidance, and performant SQL query syntax for BigQuery telemetry across INFORMATION_SCHEMA, Cloud Monitoring, and the REST API. Use when the telemetry to fetch is already known, selecting telemetry tools, writing performant INFORMATION_SCHEMA queries, retrieving telemetry for diagnosing single-job performance bottlenecks, investigating slot contention, job concurrency and queue latency, analyzing reservation capacity, utilization and autoscaling saturation, or auditing capacity-based and on-demand compute and storage resource billable usage. Don't use for root-cause diagnosis or symptom troubleshooting when the cause is unknown (use bigquery-troubleshooting first), or for writing or optimizing business logic SQL (use bigquery-optimization).

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Configures best-practice, high-signal alerting policies for Cloud Run resources on Google Cloud (services, jobs, and worker pools) based on seasoned SRE practices. Use when analyzing, recommending, writing, or deploying Terraform PromQL alerting policies to monitor Cloud Run error rates (4xx/5xx), request latency, container instance saturation (warning/critical), container CPU/memory utilization and allocation, billable instance time, job execution status, and worker pool queue backlog. Don't use for GKE workloads (use gke-alert-configuration) or Compute Engine VMs.

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Neubrutalism design system skill. Use when building bold UI with thick borders, offset solid shadows, high saturation colors, and minimal border radius.

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Run stepped HTTP load tests with ab/wrk, ramping concurrency levels to collect p50/p90/p99 latency, detect performance inflection points, and recommend optimal concurrency. Triggered by requests like 'load test this URL', 'benchmark my API', 'find the max concurrency', or mentions of p99 latency, throughput saturation, or capacity planning.

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Agent E2 - Qualitative Coding Specialist - Systematic coding and theme development. Covers codebook development, coding strategies, saturation assessment, and CAQDAS guidance.

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Runs maximum-saturation research with a cooperating team, claim-graph gating, and a cited, QA'd deliverable. Use when the user explicitly asks for research or a deep investigation, including any 'ulw' research wording.

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[omh] Deep dive before a decision: deep research engine - grounding for specs and decisions: study open-source reference implementations with pinned refs, gather live web evidence with citation discipline, verify contested claims, and distill a decision-grounding dossier that planning consumes; for a decision brief use research-brief, for upstream guidance use web-research. Use when the user says: research plan, literature review, research literature, review recent papers, deep research, deep-research, exhaustive research, saturation research.

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Score, annotate, and analyze the functional impact of genetic variants using AlphaGenome Variant Impact (AVI) scores. Query variants in chr:pos:ref>alt format, annotate VCF/tabular callsets, perform saturation mutagenesis window scans (1-based closed chr:start-end), and extract GENCODE v46 GTF gene/exon/junction coordinates all via the AlphaGenome Atlas API.

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Adapt an existing palette to dark mode — surface elevation, contrast rebalancing, and desaturation rules. Use when you already have a light palette to translate. For building the base palette first, use `color-system`.

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Use this skill when the user asks to summarize, or asks why Simulink Coverage objectives are missing, unsatisfied, or uncovered on a coverage result they already have — why specific decision, condition, MCDC, relational-boundary, or saturation/overflow outcomes weren't exercised, whether uncovered logic is dead code, or how to close the gap (a test, a coverage filter, or a design change). It reads an existing cvdata, .cvt file, sim-with-coverage output, or Simulink Test result; it never runs cvsim to collect coverage.

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Designs and analyzes pooled prime-editor (PE) screens for installing precise genetic variants without bystander confounding. Covers pegRNA design with PRIDICT and PRIDICT2 for predicting per-pegRNA editing efficiency, pegRNA architecture (spacer + scaffold + PBS + RTT), PE2/PE3/PE3b/PEmax variants, MOSAIC in situ saturation mutagenesis, the PRIME pooled-screen methodology (Ren 2023; ~3,699 ClinVar variant screens), chromatin context as a major locus-level determinant of PE efficiency, scaffold-incorporation and indel byproduct quantification with CRISPResso2, and the cross-modal validation strategy of PE + base-editor screens for variant function. Use when designing a pegRNA library for variant installation, choosing between BE and PE for a specific edit, predicting pegRNA efficiency before library synthesis, analyzing PE screen output, distinguishing intended-edit from scaffold-incorporation, or scaling PE screens to thousands of variants.

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Analyzes base-editing screens for variant function. Covers library design (Hanna 2021 ClinVar-scale CBE screen benchmarked on BRCA1/2, Cuella-Martin 2021 DDR saturation), CBE vs ABE chemistry choice (BE3/BE4 vs ABE7.10/ABE8.20/ABE8e), editing-window math (positions 4-8 from PAM-distal end; 4-7 for ABE7.10), bystander-edit quantification and the variant-call ambiguity it creates, sgRNA-efficiency filtering before hit calling, indel byproduct interpretation, the substitution-vs-indel diagnostic, variant annotation against ClinVar / COSMIC, and the Broad be-validation-pipeline. Use when designing a BE variant screen, choosing CBE vs ABE for a specific edit, interpreting bystander-confounded hits, distinguishing functional signal from indel artifact, integrating CRISPResso2 output with screen scoring, or deciding BE vs PE for SNV installation.

日本語の概要は準備中です。原文の説明を表示しています。