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Retrieve protein and nucleotide sequences from NCBI databases using E-utilities. Supports direct accession lookup, CDS translation, gene+organism search, locus lookup, PubMed-linked sequences, patent protein extraction, and organism+length fallback search. Use when you need to fetch biological sequences by accession, gene name, locus tag, PubMed ID, or patent number.
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Read biological sequence files (FASTA, FASTQ, GenBank, EMBL, ABI, SFF) using Biopython Bio.SeqIO. Use when parsing sequence files, iterating multi-sequence files, random access to large files, or high-performance parsing.
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Use when the user asks to "build a B2B cold-outbound sequence", "design reply-triage branching", "plan a domain warmup / sending throttle", or "make my outbound CAN-SPAM / opt-in compliant"; produces a multi-step outbound sequence with reply-triage branches (positive / objection / referral / not-now / opt-out), a warmup + send-throttle ramp schedule, jurisdiction opt-in/CAN-SPAM guardrails (guidance, not legal advice), and a SEND S-dimension read. Not for B2C lifecycle flows — use email-sequence-designer; not for the consent record — use consent-registry; not for computing EQS — use email-quality-auditor. B2B冷启动外联序列/回复分流/域名预热
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Implement Apollo.io email sequences and outreach workflow. Use when building automated email campaigns, creating sequences, or managing outreach through Apollo. Trigger with phrases like "apollo email sequence", "apollo outreach", "apollo campaign", "apollo sequences", "apollo automated emails".
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Build multi-sequence email automation flows with branching logic. Triggers on: "build email automation", "create email funnel", "email automation flow", "welcome series with branches", "conditional email sequence", "set up automation", "email workflow builder", "segmented email flow", "advanced email sequence", "nurture funnel", "cart abandonment sequence", "win-back email flow".
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Analyze DNA/RNA/protein sequences. Use when the user provides a sequence and asks for analysis, translation, GC content, ORFs, motifs, restriction sites, or primer design. Triggers on "sequence", "translate", "GC content", "ORF", "primer", "restriction", "complement", "reverse complement".
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Access UniProt for protein sequence and annotation retrieval. Use this skill when: (1) Looking up protein sequences by accession, (2) Finding functional annotations, (3) Getting domain boundaries, (4) Finding homologs and variants, (5) Cross-referencing to PDB structures. For structure retrieval, use pdb. For sequence design, use proteinmpnn.
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ESM2 protein language model for embeddings and sequence scoring. Use this skill when: (1) Computing pseudo-log-likelihood (PLL) scores, (2) Getting protein embeddings for clustering, (3) Filtering designs by sequence plausibility, (4) Zero-shot variant effect prediction, (5) Analyzing sequence-function relationships. For structure prediction, use chai or boltz. For QC thresholds, use protein-qc.
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Build Tier 1/2/3 cold outreach sequences differentiated by personalization depth and research signal strength during PRD v0.9 Go-to-Market. Triggers on requests to plan cold outreach, founder-led sales, or when user asks "cold email sequence", "outbound campaign", "founder outreach", "LinkedIn outreach", "Tier 1 personalization", "predictable revenue", "sales cadence", "BDR sequence". Outputs GTM-OUT-* sequence entries and lead-list references.
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Designs automated email sequences end to end: the trigger, branches and exit rules, the full copy of every message, and the sender setup that keeps them out of spam (SPF, DKIM, DMARC, one-click unsubscribe, consent). Covers onboarding and welcome series, lead nurture, abandoned cart, dunning, win-back and sunset flows. Use when someone asks for a "drip campaign", "welcome sequence", "onboarding emails", "nurture sequence", "win-back emails", "lifecycle emails", "email automation", or says "our emails land in spam" or "nobody clicks our onboarding emails". In-app onboarding belongs to onboarding-cro.
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When the user wants to create or optimize an email sequence, drip campaign, automated email flow, or lifecycle email program. Also use when the user mentions "email sequence," "drip campaign," "nurture sequence," "onboarding emails," "welcome sequence," "re-engagement emails," "email automation," "lifecycle emails," "trigger-based emails," "email funnel," "email workflow," "what emails should I send," "welcome series," or "email cadence." Use this for any multi-email automated flow. For cold outreach emails, see cold-email. For in-app onboarding, see onboarding.
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Write B2B cold emails and follow-up sequences that get replies. Use when the user wants to write cold outreach emails, prospecting emails, cold email campaigns, sales development emails, or SDR emails. Also use when the user mentions "cold outreach," "prospecting email," "outbound email," "email to leads," "reach out to prospects," "sales email," "follow-up email sequence," "nobody's replying to my emails," or "how do I write a cold email." Covers subject lines, opening lines, body copy, CTAs, personalization, and multi-touch follow-up sequences. For warm/lifecycle email sequences, see emails. For sales collateral beyond emails, see sales-enablement.
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When the user wants to create or optimize an email sequence, drip campaign, automated email flow, or lifecycle email program. Also use when the user mentions "email sequence," "drip campaign," "nurture sequence," "onboarding emails," "welcome sequence," "re-engagement emails," "email automation," "lifecycle emails," "trigger-based emails," "email funnel," "email workflow," "what emails should I send," "welcome series," or "email cadence." Use this for any multi-email automated flow. For cold outreach emails, see cold-email. For in-app onboarding, see onboarding.
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Write B2B cold emails and follow-up sequences that get replies. Use when the user wants to write cold outreach emails, prospecting emails, cold email campaigns, sales development emails, or SDR emails. Also use when the user mentions "cold outreach," "prospecting email," "outbound email," "email to leads," "reach out to prospects," "sales email," "follow-up email sequence," "nobody's replying to my emails," or "how do I write a cold email." Covers subject lines, opening lines, body copy, CTAs, personalization, and multi-touch follow-up sequences. For warm/lifecycle email sequences, see emails. For sales collateral beyond emails, see sales-enablement.
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Conjecture generation, sequence analysis, and experimental mathematics for mathematical discovery. Covers sequence analysis (OEIS lookups, recurrence relations, generating functions), pattern detection methods (finite differences, ratio analysis, modular patterns), conjecture generation workflow, classical integer sequences (Fibonacci, Catalan, partition numbers, Bernoulli numbers), combinatorial identities, the probabilistic method (Erdos, expected value arguments, Lovasz Local Lemma), AI-assisted discovery (FunSearch, Ramanujan Machine), and confidence calibration with cautionary tales (Mertens conjecture, Polya conjecture). Use when discovering patterns, analyzing sequences, generating conjectures, or working with combinatorial identities.
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Build a complete cold email outreach system — ICP definition, sequence architecture, personalization strategy, and batch-produced email sequences with CAN-SPAM/deliverability compliance. Use when "cold outreach", "cold email sequence", "outbound campaign", "prospecting emails", "sales outreach", "build outreach sequence", or any request to create systematic cold email campaigns for lead generation.
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Build automated email flows that nurture, convert, and retain. Creates complete sequences for welcome, nurture, launch, re-engagement, and onboarding with subject lines, body copy, timing, and A/B test plans. Use when someone needs email automation, a drip campaign, welcome series, launch emails, post-purchase emails, abandoned cart recovery, or says 'email sequence', 'drip campaign', 'welcome series', 'onboarding emails', 'nurture flow', 'automated emails', 'email marketing', 'retention emails', or 'lifecycle emails'. Even if they just say 'set up emails' or 'email strategy' without specifying 'sequence', this is likely the right skill. Includes deliverability rules and spam avoidance.
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Email marketing operations and automation for B2B SaaS. Use this skill for newsletter strategy, email automation sequences, lifecycle campaigns, nurture workflows, onboarding sequences, deliverability optimization, email copywriting, lead scoring, marketing automation platform setup, and cold-email sending infrastructure — secondary sending domains, SPF/DKIM/DMARC, and domain warmup — plus the contact budget: a cross-channel frequency cap across every sender (marketing, sales sequences, customer success, in-app). Also triggers on: email, newsletter, drip campaign, lifecycle, nurture, onboarding sequence, deliverability, email automation, lead scoring, marketing automation, our emails are going to spam, SPF, DKIM, DMARC, sender reputation, sending domain, cold email domains, domain warmup, mailbox setup, blacklist, bounce rate, BIMI, brand logo in inbox, VMC, Verified Mark Certificate, Common Mark Certificate, transactional email, password reset emails going to spam, DMARCbis, RFC 9989, DMARC pct tag, DMARC t=y test mode, DMARC rollout, move DMARC to reject, frequency cap, email fatigue, customers get too many emails, too many messages, sales and marketing both emailing the same person, communication limits, send frequency safeguard, global frequency capping, quiet period, message precedence, AI email summaries, Apple Intelligence summary replaces preview text, Gmail Gemini summary, Gmail AI Inbox, Apple Mail categories, our emails land in Promotions, writing emails for AI summaries.
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Use when the user asks to "build a B2B cold-outbound sequence", "design reply-triage branching", "plan a domain warmup / sending throttle", or "make my outbound CAN-SPAM / opt-in compliant"; produces a multi-step outbound sequence with reply-triage branches (positive / objection / referral / not-now / opt-out), a warmup + send-throttle ramp schedule, jurisdiction opt-in/CAN-SPAM guardrails (guidance, not legal advice), and a SEND S-dimension read. Not for B2C lifecycle flows — use email-sequence-designer; not for the consent record — use consent-registry; not for computing EQS — use email-quality-auditor. B2B冷启动外联序列/回复分流/域名预热
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在React或Next.js中实现拖动、滑动手势、文字和SVG动画，涵盖多阶段动作、滚动与光标跟随及加载状态，并帮助按用途选择API。
根据开发目标选择ECC命令组，说明执行顺序与停止条件，并读取当前命令文件，按用途整理清单，帮助组合多个命令；仅提供指导，不执行命令。
帮助使用GSAP编排多个动画的顺序与重叠，指定起始位置、嵌套动画组，并实现播放、暂停、倒放及跳转到指定位置。
Build coordinated animation sequences with GSAP timelines. Set when each movement starts, overlap effects, name key moments, combine smaller sequences, and control playback, including pause, reverse, restart, and jumps to a specific point.
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Design and review PCR and RT-qPCR primers, short DNA sequences used to start amplification. Assess predicted structures and unintended products under explicit reaction conditions, with reports that track reference sequences, coordinates, and search limits.
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