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概要と使いどころ
Perform differential expression analysis using DESeq2 in R/Bioconductor. Use for analyzing RNA-seq count data, creating DESeqDataSet objects, running the DESeq workflow, and extracting results with log fold change shrinkage. Use when performing DE analysis with DESeq2.
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Read and manipulate Flow Cytometry Standard (FCS) files. Covers loading data, accessing parameters, and basic data exploration. Use when loading and inspecting flow or mass cytometry data before preprocessing.
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Analyze time-series RNA-seq data using limma voom with splines, maSigPro, and ImpulseDE2. Identify genes with dynamic expression patterns. Use when analyzing time-series or longitudinal expression data.
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Query dbSNP for rsID lookups, variant annotations, and cross-references to other databases. Use when mapping between rsIDs and genomic coordinates or retrieving basic variant information.
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Stops defensive writing across the entire paper lifecycle — writing, revising, cutting, and organizing experiments. Treats the paper as a press conference, not a project summary, lab log, or self-audit: identify the single most publishable strength of the work and build the most favorable, complete, and persuasive narrative around it. Never present everything evenly, never volunteer weakness, never write an experiment diary, never attack the paper on the reviewer's behalf. Triggers: polish my paper, revise manuscript, write abstract, write introduction, write conclusion, shorten paper, rebuttal, respond to reviewers, organize experiments, defensive writing, AI-flavored academic writing.
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Autonomously screen NYSE, Nasdaq, and NYSE American operating-company stocks for undervalued-growth/GARP opportunities using forward same-basis valuation, driver-derived EPS/FCF forecasts, primary-source financial verification, SBC and dilution controls, sector and cycle normalization, auditable candidate-pool coverage, and fail-closed final reporting. Use when asked to find, screen, rank, or refresh US undervalued-growth stocks, including minimal requests with no ticker list or parameters.
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Use when the user asks to "build programmatic SEO pages", "generate pages at scale", "rank on a high-authority third-party site", "borrow domain authority", "build a vs / alternative page", "do local SEO", "optimize a Google Business Profile", or "fix NAP" — one page-build router with four modes: programmatic (template × dataset systems), parasite (barnacle publishing on Medium/Reddit/LinkedIn/GitHub), comparison (vs / alternative pages in four formats), and local (GBP + NAP + citations + location pages), each with its own thin/duplicate, honesty, or reputation-abuse guardrail. Not for finding what to target — use keyword-research or content-gap-analysis; not for drafting one standalone article — use content-writer; not for scoring a page for publish — use content-quality-auditor. 程序化SEO/批量页面/寄生SEO/借势权重/对比页/vs页/替代页/本地SEO/谷歌商家档案/NAP一致性
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Use when the user asks to "log this subscriber's opt-in", record unsubscribes/complaints, or query lawful basis; curates pseudonymous consent facts through the append-only consent stream and applies suppression/erasure tombstones immediately. Not for SEND scoring — use email-quality-auditor; not for building segments — use list-segment-builder. 订阅同意台账/实时退订抑制/合法性依据登记
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Critique a rendered screen's hierarchy — entry point, eye flow, weight distribution, and emphasis. Use when attention lands in the wrong place. For establishing hierarchy in new work, use `visual-hierarchy` (ui-design).
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Critique a rendered screen's composition — balance, whitespace, rhythm, and gestalt grouping. Use when a layout feels off but hierarchy is fine. For emphasis and eye flow specifically, use `critique-visual-hierarchy`.
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Apply the Serial Position Effect — first and last items in a sequence are recalled best. Use when ordering menus, lists, and steps. For emphasising one item regardless of its position, use `von-restorff-effect` (ui-design).
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Apply the Von Restorff Effect — the element that differs from its neighbours is the one remembered. Use when a single action must dominate. For overall ordering rather than single-element emphasis, use `visual-hierarchy`.
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Apply the Law of Figure-Ground — establish which layer is foreground and actionable versus background. Use when designing modals, overlays, and depth. For emphasising one element among peers, use `von-restorff-effect`.
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Define motion tokens — durations, easing vocabulary, and reduced-motion handling — for consistency product-wide. Use when standardising motion across a system. For crafting one specific animation, use `animation-principles` (interaction-design).
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Build the project's shared language and bounded contexts before writing code, so names stay consistent and the agent stops paraphrasing domain concepts. Produces a CONTEXT.md glossary and decision records. Use at the start of a project or feature, or when the codebase and the people describing it speak different languages.
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Apply interface craft when building or reviewing UI - motion, easing, timing, springs, component feel, and visual foundations. Use when building a component, animation, transition, hover or press state, modal, drawer, toast, or when polishing an interface so it feels right. Says "make this feel better", "add an animation", "polish the UI", "review this component".
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Guides building full-stack applications with AWS Blocks — an Infrastructure-from-Code framework. Applies when creating APIs, selecting Building Blocks (KVStore, DistributedTable, Database, AuthBasic, AuthCognito, Realtime, AsyncJob, FileBucket, etc.), running local development, or deploying AWS Blocks applications. Also covers AWS Blocks topics with validated, version-specific patterns that prevent common mistakes. Triggers when user mentions AWS Blocks; project has aws-blocks/ directory; code imports @aws-blocks packages.
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Activate when developers have latent caching needs: slow API responses, database read bottlenecks, DynamoDB throttling or cost, RDS/Aurora scaling pressure, Bedrock latency or cost, or adding a cache; activate when working with Redis, Valkey, Memcached, or any in-memory data store, cache-aside patterns, session stores, rate limiting, leaderboards, counters, streams, queues, pub/sub, distributed locks, feature flags, shopping carts, or other caching strategies. Activate for GenAI and ML retrieval: vector similarity search for low-latency retrieval, semantic caching, RAG, LLM response caching, embedding stores, AI agent memory, recommendation, personalization. Activate for ElastiCache lifecycle: provisioning (serverless or node-based), engine selection, CloudFormation/CDK/Terraform IaC, VPC connectivity, TLS, RBAC, IAM auth, Global Datastore, monitoring, troubleshooting, cost optimization, and migration from self-managed Redis. Do not trigger for browser caches, CDN/CloudFront, HTTP Cache-Control, CPU caches.
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Entry point and router for every Expo or EAS task. Load this skill first — before writing code and before choosing another expo-* / eas-* skill — when the request, PRD, or spec mentions Expo, EAS, Expo Go, or an expo-* package, or the project has an `expo` dependency in `package.json`. Within that gate it also covers app specs and designs to implement (tabs, stacks, maps, lists, navigation, building from a screenshot), and phrasings like 'implement a mobile app', 'make my app look native', 'add navigation', 'fetch some data', 'upgrade my SDK', 'add Expo to my existing native app', 'ship to the App Store', or 'I'm new to Expo, where do I start'. A fully specified request (SDK pinned, libraries named, layout given) still routes through here — the shared setup rules still apply. Do NOT load it when neither signal is present: a bare React Native project with no `expo` dependency is not Expo work. Detects the real goal, routes to the right expo-* / eas-* skill, and owns the shared setup rules.
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Analyze and apply AssemblyAI secret, region, consent, retention, deletion, and model-training controls. Use when reviewing security or regulated speech workflows. Trigger with "AssemblyAI security", "AssemblyAI privacy", or "data retention".
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Harden an Attio integration with least-privilege credentials, tenant isolation, log redaction, raw-body webhook verification, idempotency, and incident-ready rotation. Use when reviewing Attio security controls or preparing production access. Trigger with "Attio security", "secure Attio integration", or "Attio webhook signature".
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Apply security best practices for Apple Notes automation scripts. Trigger: "apple notes security".
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Plan and execute a staged deployment for an external Attio REST integration with secret injection, contract tests, read-only smoke evidence, canary writes, and rollback. Use when releasing Attio-backed services. Trigger with "deploy Attio integration", "Attio rollout", or "Attio release plan".
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Secure AppFolio API credentials and tenant data. Trigger: "appfolio security".
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