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Check, from the employer's side, what has been put in writing before a paid Otameshi Tenshoku assignment starts under gyomu-itaku: item by item whether the deliverable, delivery date, fee and how it is calculated, payment due date, who pays, out-of-scope work, revision limits, company support, rights, confidentiality and termination are stated in a contract, purchase order or email rather than in chat or by word of mouth, count the days from delivery to payment, and find pay tied to satisfaction or hiring, unpaid or unlimited revisions and one-sided termination. Use when a hiring manager, recruiter or small-business owner is about to offer terms to a candidate, has been asked by a candidate what is still unwritten, or needs the conditions written for a platform contract screen; do not use to judge whether the terms are lawful or the fee fair, to compute withholding or consumption tax, for employment contracts, or to sign, send or conclude the contract on the user's behalf.

日本語の概要は準備中です。原文の説明を表示しています。

Read a Japanese job posting closely and report what it actually states: base pay separated from fixed overtime allowance, the range converted to an hourly basis, required versus preferred requirements, and the terms left ambiguous. Use when a user wants a 求人票 or job description examined before applying, wants two postings compared on the same basis, or wants questions prepared about pay and working conditions; do not use to judge whether the user will be hired, to infer duties the posting does not state, or to evaluate the employer's reputation.

日本語の概要は準備中です。原文の説明を表示しています。

Put two or more Japanese job offers on one basis so they can be read side by side, separating fixed overtime allowance and unguaranteed bonus from the stated annual figure, converting each offer to guaranteed annual pay and to an hourly rate that includes deemed overtime hours, and listing non-monetary terms without scoring them. Use when a user holds more than one offer, wants to know what a stated 年収 actually contains, or wants to see where the ranking changes once the components are separated; do not use to recommend which offer to accept, to score or rank offers, to estimate take-home pay as fact, or to reply to an employer.

日本語の概要は準備中です。原文の説明を表示しています。

Prepare a request to change the terms of a Japanese job offer before accepting it, sorting what the user wants changed (pay components, start date, work style, location, title, probation) by priority, tying each ask to a basis the user can show (the written offer, the job posting, what was said in interviews, their own records), working the timing back from the acceptance deadline, and drafting the message to the employer or recruiting agent. Use when a user has an offer and wants to ask for higher pay, a later start date, more remote days, or other changed terms, or wants to know how to raise it without losing the offer; do not use to predict whether the request will succeed, to set a target figure from market rates, to fabricate a competing offer, to decide whether to accept, or to send anything to the employer.

日本語の概要は準備中です。原文の説明を表示しています。

Work out the order and the loose ends before turning down an offer or withdrawing from a selection process: whether the offer being kept is actually settled in writing, whether each decline is mid-selection, post-offer, or after acceptance, who besides the employer has to be told when the application came through an agent, a school recommendation, or a referral, which reply deadlines have passed, and what documents, expenses, or borrowed items are still outstanding. Use when a user holds more than one offer and needs to decline the rest, wants to withdraw from a process they are no longer pursuing, or has been asked to turn down other employers as a condition; do not use to decide whether to decline, to rule on what declining after acceptance means legally, or to send any message.

日本語の概要は準備中です。原文の説明を表示しています。

Help a user talk through whether to change jobs, stay, or change something in their current work, starting from uncertainty, distress, a vague sense of stagnation, an offer in hand, a counter-offer from the current employer, or pressure from family or colleagues. Clarify what matters, separate deadlines that order the next steps from the decision itself, compare conditional options, and agree on a small next step and a time to revisit it. Use when someone asks whether they should quit, wonders whether leaving is just running away, has second thoughts after receiving an offer or being asked to stay, feels stuck because others say it would be a waste, wants to leave soon after joining, or wants advice before deciding to start a job search; do not make the final stay-or-leave decision, evaluate offer terms, plan the resignation, or predict hiring outcomes.

日本語の概要は準備中です。原文の説明を表示しています。

Check the working conditions of a Japanese job offer item by item against the conditions an employer states in writing, compare the offer letter or 労働条件通知書 with the job posting and what was said in interviews, and separate what is confirmed in writing from what is still only verbal. Use when a user has received an offer, a working-conditions notice, or an employment contract and wants to know what is missing, what conflicts with earlier statements, and what to confirm before the acceptance deadline; do not use to rule on whether the terms are lawful, to decide whether to accept or decline, or to contact the employer on the user's behalf.

日本語の概要は準備中です。原文の説明を表示しています。

Assemble a Japanese shokumu-keirekisho (career history document) from an experience inventory, choosing between reverse-chronological, chronological, and skills-based layouts, mapping the target posting's requirements to the experiences that can support them, and drafting the summary, work history, skills, and self-PR so that each sentence stays within what its evidence supports and confidential details are abstracted. Use when a user wants to write or rebuild a whole shokumu-keirekisho for a specific posting or as a general version, or wants to decide which experiences to include and in what order; do not use to invent experience, numbers, titles, or qualifications, to predict whether it will pass screening, to fill in a rirekisho form, or to submit it anywhere.

日本語の概要は準備中です。原文の説明を表示しています。

Draft or review an employer's Otameshi Tenshoku listing so a candidate can decide whether to apply: check item by item that the work, deliverable, out-of-scope tasks, required experience, support, hours, schedule, location, compensation, contract form and payment timing are stated, find open-ended, unpaid-company-work, guarantee, personal-attribute and unverifiable wording, and confirm that the hours, weekly availability, period, rate and budget hold together. Use when a hiring manager, recruiter or small-business owner wants a listing written from a plan, wants an existing draft or published listing reviewed before or after posting, or wants to know why applications are not coming; do not use to design the work itself (trial-work-design), to reply to applicants (applicant-reply), to state market rates, legal, tax or service conditions from memory, or to post or update the listing on the user's behalf.

日本語の概要は準備中です。原文の説明を表示しています。

Plan the first months at a new Japanese employer after an offer is accepted, listing every expectation and promise made in interviews, the offer, or negotiation with its source, separating what is in writing from what was only said and what has no agreed measure, working out when the probation period ends and whether its criteria and conditions are known, and scheduling who to confirm what with before and after the start date. Use when a user has accepted an offer and wants to prepare for joining, make sure verbal promises such as remote days or a future role are confirmed, or understand what the probation period means for them; do not use to predict whether they will fit in or pass probation, to judge their manager or colleagues, to rule on the legality of probation terms, or to contact the new employer.

日本語の概要は準備中です。原文の説明を表示しています。

Philosophy-first writing partner for Medium with tactical depth. Combines human storytelling values with production-ready technical content tools. Six content modes based on staff pick analysis, mode-specific quality gates, evidence requirements, annotated examples, and comprehensive scripts integration. Designed for sustainable practice, not algorithm gaming.

日本語の概要は準備中です。原文の説明を表示しています。

Identify the first beachhead market segment for a product launch. Evaluates segments against burning pain, willingness to pay, winnable market share, and referral potential. Use when choosing a first market, targeting an initial customer segment, or planning market entry strategy.

日本語の概要は準備中です。原文の説明を表示しています。

Gate a build on browser fingerprint regressions with liarjs - save a baseline scan as JSON, diff later runs against it, and fail the job when the consistency score falls below a floor. Use when asked to add a fingerprint or headless-detection check to GitHub Actions, GitLab CI or another pipeline, to catch a regression in a Chromium build or scraping harness before it ships, or to track how a fingerprint score changes across commits.

日本語の概要は準備中です。原文の説明を表示しています。

Infer or annotate TCR antigen specificity by unsupervised clustering (TCRdist/tcrdist3, GLIPH2, clusTCR, GIANA) and database lookup (VDJdb, IEDB, McPAS-TCR), and rank candidates with supervised predictors (ERGO-II, NetTCR-2.x, pMTnet) under explicit caveats. Encodes the central truth that general TCR-epitope prediction for UNSEEN epitopes essentially does not work (collapses to near-random; IMMREP22, Grazioli 2022) because labeled data is dominated by a few immunodominant epitopes and there is no true negative set — so clustering for discovery is the honest task and de-novo binding needs wet-lab validation. Use when annotating TCR specificity or grouping a repertoire. Epitope/MHC context lives in mhc-binding-prediction.

日本語の概要は準備中です。原文の説明を表示しています。

Predicts protein-coding gene structures (exons, introns, UTRs) in eukaryotic genomes with BRAKER3 (RNA-seq + protein evidence), BRAKER1/BRAKER2, GALBA (protein-only), Funannotate (fungi), GeMoMa (homology projection), or Helixer/Tiberius (deep-learning ab initio). Covers the evidence-first tool decision, mandatory soft-masking, the training-set-quality-dominates principle, OrthoDB clade-partition selection, the one-isoform-per-locus and missing-UTR traps, merge/split errors, and reference bias against orphan genes. Use when annotating a newly assembled eukaryotic genome, choosing a gene-prediction pipeline based on available evidence, or diagnosing a poor annotation.

日本語の概要は準備中です。原文の説明を表示しています。

Performs proximity operations on genomic intervals with bedtools (closest, window, flank, slop) and pybedtools - nearest-feature queries with signed/strand-aware distance, fixed-radius window searches, strand-aware promoter construction, and interval extension. Covers the closest -d/-D a/b/ref/-t/-k/-io/-iu/-id flags, the -D ref strand sign-flip, silent chromosome-end clipping in slop/flank, -t all tie double-counting, and the critical distinction between a geometry answer (nearest TSS) and a biology answer (which gene an element regulates). Use when assigning peaks or variants to genes, defining promoters from a gene model, building distance-to-TSS distributions, finding features within a window, or extending intervals - and when deciding whether nearest-gene is a fair prior (GWAS locus) or a trap (distal enhancer).

日本語の概要は準備中です。原文の説明を表示しています。

Detects and removes contamination in genome assemblies via two disjoint workflows - foreign-sequence screening of a single-organism (eukaryote/isolate) assembly with NCBI FCS-GX (GenBank-submission-mandatory), FCS-adaptor, and BlobToolKit blob plots; and MAG/bin quality assessment with CheckM2 plus GUNC (chimerism) plus GTDB-Tk taxonomy, judged against MIMAG. Covers why CheckM2 alone is blind to disjoint-marker chimeras, the FCS-GX RAM wall, organelle/NUMT triage, strain heterogeneity, and the HGT-vs-contamination (tardigrade) trap. Use when screening an assembly for foreign contamination before GenBank submission, assessing MAG completeness/contamination/chimerism, deciding which contigs to remove, or distinguishing real HGT from contaminant contigs.

日本語の概要は準備中です。原文の説明を表示しています。

Computes and interprets sequencing read depth and coverage over a genome, windows, or target regions with mosdepth (windowed depth, cumulative distribution, --quantize callable BEDs), bedtools genomecov/coverage (bedGraph tracks, per-target stats), samtools depth/coverage (per-base depth, per-contig depth+breadth). Covers the breadth-vs-mean distinction, the cumulative-coverage curve, evenness (CV/Fano/fold-80/Gini), what each tool silently counts (duplicates, secondary/supplementary, MAPQ, read span vs fragment, mate-overlap), the samtools-depth 8000-cap version trap, and the bedtools coverage -a/-b orientation flip. Use when assessing sequencing adequacy, building coverage tracks, computing breadth at a depth threshold, defining callable regions, or QCing target-capture uniformity.

日本語の概要は準備中です。原文の説明を表示しています。

Assigns GO terms, Pfam/InterPro domains, KEGG orthologs, EC numbers, and product names to predicted proteins using eggNOG-mapper (orthology), InterProScan (domain signatures), and KofamScan (KEGG), routing specialized functions to dbCAN/antiSMASH/AMRFinderPlus/SignalP. Covers the orthology-vs-domain-vs-homology paradigms, the annotation-error percolation cascade, domain-presence-is-not-function, GO IEA circularity in enrichment, evidence tiering, and bit-score/coverage thresholds. Use when adding functional annotation to predicted genes, choosing between eggNOG-mapper and InterProScan, or judging how much to trust a functional label.

日本語の概要は準備中です。原文の説明を表示しています。

Renders Hi-C contact matrices honestly and reproducibly with matplotlib, cooltools, HiCExplorer, pyGenomeTracks, FAN-C, CoolBox, and plotgardener. Covers the raw/ICE-balanced/observed-over-expected transform choice, LogNorm vs symmetric-diverging colormaps with vmax/percentile clipping, resolution-to-feature matching (compartments 100-500kb, TADs 10-40kb, loops 5-10kb), square vs rotated-triangle track-stacking, NaN/white-stripe handling, virtual 4C, APA/saddle/on-diagonal pileups, two-condition side-by-side and log2-ratio maps, and interactive (HiGlass) vs scripted-static publication figures. Use when plotting a contact matrix, choosing a normalization or color scale, building a multi-track Hi-C figure, making a virtual 4C profile, piling up loops/boundaries, or comparing two conditions.

日本語の概要は準備中です。原文の説明を表示しています。

Decides whether and how to polish a draft genome assembly to raise consensus accuracy (QV) with read-type-matched tools - Racon and medaka (ONT consensus), dorado polish, Polypolish and pypolca (Illumina, repeat-aware), Pilon (legacy short-read), NextPolish/NextPolish2, Hapo-G (haplotype-aware), ntEdit, and DeepPolisher/PEPPER-Margin-DeepVariant for human. Covers the do-not-polish-HiFi rule, the medaka basecaller-model footgun, held-out Merqury QV as the only honest stop signal, and the haplotype-collapse trap. Use when correcting homopolymer indels or residual SNPs in a long-read assembly, deciding if a HiFi assembly needs polishing, or choosing an ONT vs hybrid vs short-read polishing chain.

日本語の概要は準備中です。原文の説明を表示しています。

Identifies non-coding RNAs (tRNA, rRNA, snoRNA, snRNA, riboswitches, sRNAs) using Infernal covariance-model search against Rfam, tRNAscan-SE 2.0 for tRNA, barrnap for rRNA, and ARAGORN for tmRNA, plus the small-RNA-seq boundary for miRNA and the transcript-assembly boundary for lncRNA. Covers the structure-conserved-not-sequence-conserved principle (why BLAST fails), GA-threshold and clan-competition correctness, tRNAscan-SE domain modes and pseudogene flags, rDNA copy-number collapse, and why homology annotation is a recall floor. Use when performing genome-wide ncRNA annotation, choosing the right tool for an RNA class, or interpreting ncRNA counts.

日本語の概要は準備中です。原文の説明を表示しています。

Build enhancer-driven gene regulatory networks (eGRNs) by integrating single-cell RNA-seq and ATAC-seq using SCENIC+, CellOracle base GRNs, Pando, FigR, DIRECT-NET, TRIPOD, and scMEGA. Covers the accessibility-defines-enhancers principle, peak-to-gene linking and its cell-composition confound, the paired-vs-unpaired decision, and TF-region-gene eRegulon triplets. Use when analyzing 10x multiome or paired/unpaired scRNA+scATAC to infer cis-regulatory GRNs. For RNA-only regulons see scenic-regulons; for in silico TF perturbation see perturbation-simulation.

日本語の概要は準備中です。原文の説明を表示しています。

Bead-based signal normalization and cross-batch harmonization for CyTOF and high-parameter cytometry - EQ four-element bead normalization of instrument sensitivity drift (CATALYST normCytof, premessa), and reference-anchor cross-batch normalization (CytoNorm, per-cluster quantile splines). Covers the distinction between within-run drift correction and between-batch correction, the mandatory anchor/reference sample, why normalization is per-cluster with many quantiles, and the over-correction risk. Use when correcting CyTOF signal drift, harmonizing multi-batch or multi-site studies, or deciding whether to normalize data versus model batch in the design.

日本語の概要は準備中です。原文の説明を表示しています。