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PCBWay PCB fabrication and assembly — turnkey/consigned assembly, design rules, ordering workflow. Alternative to JLCPCB for manufacturing. Use with KiCad. Use this skill when the user mentions PCBWay, needs turnkey assembly (PCBWay sources parts by MPN), has parts not available on LCSC, needs assembled boards with non-LCSC components, wants to compare PCBWay vs JLCPCB, or needs assembly with parts sourced globally rather than from LCSC only. For gerber/CPL export, stencil ordering, and BOM management, see the `bom` skill.

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Assembles a genome de novo from Illumina short reads with SPAdes (isolate/careful/sc/meta/plasmid/rna modes), MEGAHIT (low-memory, huge datasets), Unicycler (bacterial finishing/hybrid), MaSuRCA (large hybrid), ABySS (Bloom-filter), and Platanus (heterozygous diploids), using multi-k de Bruijn graphs. Covers the repeat-resolution limit, why N50 plateaus at the genome not the depth, GenomeScope2 k-mer profiling first, the heterozygosity/haplotig trap, error-correction erasing rare alleles, GC dropout, and NG50/auN/BUSCO reporting. Use when assembling a bacterial isolate, fungal, small-eukaryotic, single-cell, or metagenome genome from Illumina reads, or when deciding whether short reads can even produce the assembly being asked for.

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Assembles microbial-community sequencing into metagenome-assembled genomes (MAGs) with metaFlye (ONT), metaSPAdes/MEGAHIT (Illumina), and hifiasm-meta/metaMDBG (PacBio HiFi), then recovers genomes via multi-binner consolidation (MetaBAT2, MaxBin2, CONCOCT, SemiBin2, VAMB -> DAS_Tool) and QCs them against MIMAG with CheckM2, GUNC, and GTDB-Tk. Covers why a metagenome is not a genome (uneven coverage, micro-diversity, strain collapse to consensus), differential-coverage binning, co-assembly vs per-sample, the rRNA-operon collapse that fails short-read MAGs, and strain resolution with inStrain. Use when reconstructing genomes from a microbiome, soil, ocean, or gut community, recovering MAGs, or resolving strain-level variation.

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Assembles haplotype-resolved diploid and telomere-to-telomere (T2T) genomes from PacBio HiFi reads with hifiasm (HiFi-only, Hi-C, or trio phasing) and verkko (HiFi + ultralong ONT for T2T), extracting contigs from GFA and routing phasing QC to k-mer/trio metrics. Covers why a primary assembly is a haplotype mosaic that exists in no cell, partial-vs-full phasing (the .bp. vs .dip. filename convention), the purge-default trap on inbred samples, the --hom-cov coverage-estimate alarm, and verkko-vs-hifiasm for T2T. Use when assembling a diploid eukaryote from HiFi, phasing haplotypes with parents (trio) or Hi-C, deciding whether to chase T2T, or diagnosing switch errors invisible to N50/BUSCO/QV.

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Decides whether and how to polish a draft genome assembly to raise consensus accuracy (QV) with read-type-matched tools - Racon and medaka (ONT consensus), dorado polish, Polypolish and pypolca (Illumina, repeat-aware), Pilon (legacy short-read), NextPolish/NextPolish2, Hapo-G (haplotype-aware), ntEdit, and DeepPolisher/PEPPER-Margin-DeepVariant for human. Covers the do-not-polish-HiFi rule, the medaka basecaller-model footgun, held-out Merqury QV as the only honest stop signal, and the haplotype-collapse trap. Use when correcting homopolymer indels or residual SNPs in a long-read assembly, deciding if a HiFi assembly needs polishing, or choosing an ONT vs hybrid vs short-read polishing chain.

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RoadRunner asset path lookup tables for map format conversions in MATLAB. Maps lane markings, signs, signals, barriers, objects, and lane types to RoadRunner asset paths. Use when converting map formats to RRHD, resolving asset paths, or assigning visual assets to HD Map objects.

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Plan and run a media pipeline for images, video, and downloadable assets. Use this skill when designing image storage and delivery, choosing formats (WebP, AVIF), setting up responsive images, picking a video host, organizing a brand asset library, or auditing a slow image pipeline. Triggers on image pipeline, asset library, DAM, image optimization, WebP, AVIF, responsive images, video hosting, image CDN, asset workflow, media management. Also triggers when images are slow, broken, or scattered across systems.

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Design formative assessments that make students' historical thinking visible — revealing whether they source, close-read, contextualise, and corroborate. Use when assessing historical thinking skills or planning a diagnostic.

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Design an adaptive assessment loop where each student response triggers the next instructional move. Use when building technology-enhanced formative assessment cycles.

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BUSseq R package fits an interpretable Bayesian hierarchical model---the Batch Effects Correction with Unknown Subtypes for scRNA seq Data (BUSseq)---to correct batch effects in the presence of unknown cell types. BUSseq is able to simultaneously correct batch effects, clusters cell types, and takes care of the count data nature, the overdispersion, the dropout events, and the cell-specific sequencing depth of scRNA-seq data. After correcting the batch effects with BUSseq, the corrected value ca

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Dung khi da co MOT master design va can ra du kich thuoc cho tat ca kenh — bang kich thuoc chuan 2025-2026 cho feed, story, reel, cover, placement ads; 3 cach xu ly scale-crop, re-compose, rebuild; quy tac safe zone va cach dat ten file. Kich hoat khi user nhac 'resize asset', 'du kich thuoc', 'adapt kich thuoc', 'bang kich thuoc chuan', 'safe zone', 'anh bi cat mat chu tren story', 'mot anh dang duoc may cho'. Khong dung cho — thiet ke moi tu concept thi dung skill 42-brief-hinh-anh; asset gap khi campaign dang chay thi dung skill 48-quick-visual-brief; liet ke toan bo asset cua campaign thi dung skill 41-campaign-asset-list.

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Use when a LIVE campaign needs a new asset the same day — creative fatigue, CTR decay, climbing frequency, or a new angle to test: stripped-down input, duplicate the winning creative and change only what is listed, a one-page template, and a hard SLA so the designer ships in 2-4 hours. Trigger on 'quick brief', 'need an asset fast', 'creative fatigue', 'swap the creative today', 'frequency is too high', 'the ad is dying and we need a new one'. Not for — planning assets before launch, see `41-campaign-asset-list-global`; a full concept brief with time to spare, see `42-image-brief-global`; resizing an existing master, see `50-asset-resize-global`; raising budget on a winner, see `55-scaling-ads-global`.

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Analyze assertion quality, depth, variety, and false confidence in existing tests. ALWAYS USE when asked about weak, shallow, trivial, always-true, self-referential, assertion-free, presence/truthiness-only, or insufficiently diverse assertions, including MSTest, Jest, pytest, and Go. DO NOT USE for direct fixes: writing-mstest-tests owns supplied MSTest assertions; code-testing owns new cases. Use test-gap-analysis when asked whether tests would catch a production change, and test-anti-patterns for general severity-ranked audits.

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Guides comprehensive controller self-assessment covering GDPR Articles 5-49 with scoring methodology and reporting format. Activate when conducting internal reviews or benchmarking maturity. Keywords: self-assessment, controller assessment, compliance questionnaire, scoring.

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Determines whether a personal data breach triggers notification obligations under GDPR Articles 33 and 34 using structured risk assessment methodology. Covers breach type classification (CIA triad), data sensitivity scoring, volume assessment, identifiability analysis, and consequence severity evaluation. References EDPB Guidelines 01/2021 with 18 breach scenarios. Keywords: breach risk assessment, GDPR, Article 33, Article 34, EDPB, notification threshold.

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Guides privacy audit risk assessment including risk universe development, inherent and residual risk scoring, control effectiveness evaluation, risk-based audit planning, heat map generation, risk appetite alignment, and audit prioritization by risk exposure. Covers the full audit risk assessment cycle from scoping through ongoing monitoring. Keywords: audit risk assessment, risk universe, inherent risk, residual risk, control effectiveness, risk-based audit planning.

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Search and inspect the installed Blueish Blender Asset Library without mutating it. Use when a user asks for a local Blender asset, node preset, Blueish asset, Asset Browser entry, or named source, or when the Router has selected a node-centric/reusable Geometry Nodes, material, simulation, rigging, compositor, or NPR route with eligible local catalogs; return compatible, attributable candidates for the Router-approved workflow.

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Report which kasetto-installed skills and MCP servers are actually being used across the AI agents on this machine, and render a branded HTML dashboard of the result. Use whenever the user asks what agent assets they actually use, which skills or MCPs are dead weight, what to prune or clean up from kasetto.yaml, why their context is bloated with unused MCP servers, whether a skill has ever been invoked, or wants a usage report, audit, or dashboard of their agent setup. Also trigger on "kst ai assets usage", "ai asset usage", "what skills do I actually use", "which MCPs are worth keeping", "audit my agent assets", "am I using all these skills", or any request to review, trim, or justify an installed agent toolkit.

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Detect contamination and assess genome quality using CheckM, CheckM2, GTDB-Tk, and GUNC for metagenome-assembled genomes and isolate assemblies. Use when checking assemblies for contamination.

日本語の概要は準備中です。原文の説明を表示しています。

Design an adaptive assessment loop where each student response triggers the next instructional move. Use when building technology-enhanced formative assessment cycles.

日本語の概要は準備中です。原文の説明を表示しています。

Design formative assessments that make students' historical thinking visible — revealing whether they source, close-read, contextualise, and corroborate. Use when assessing historical thinking skills or planning a diagnostic.

日本語の概要は準備中です。原文の説明を表示しています。

Resolve TikTok video URLs to return no-watermark/watermarked download addresses, playback addresses, and cover image addresses for saving promotional video assets or offline analysis. Trigger when users mention TikTok video download, TikTok watermark-free download, TikTok video save, download TikTok promotional video, TikTok no watermark video, TikTok video download, download TikTok video, no watermark TikTok video, save TikTok video, TikTok video link resolution. Even if the user does not explicitly mention "Nexscope", trigger this skill whenever their need involves extracting downloadable/playable video addresses from a TikTok video link.

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Design an asset library structure — naming conventions, formats, and delivery specs. Use when asked to "organize our brand assets", "define asset naming conventions", or "spec logo delivery formats".

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Assesses infrastructure fund secondaries with asset-level cash flow analysis, concession period evaluation, and regulatory risk assessment. Use when analyzing infra secondaries, evaluating infrastructure assets, or pricing infra fund interests.

日本語の概要は準備中です。原文の説明を表示しています。