1.4万 件 ・ 関連度順
概要と使いどころ
Trim PCR primers from aligned reads in amplicon-panel BAMs using samtools ampliconclip. Use when processing SARS-CoV-2 ARTIC, hereditary cancer panels, ctDNA hot-spot panels, or any amplicon assay where primer-derived bases would falsely confirm reference at primer footprints.
日本語の概要は準備中です。原文の説明を表示しています。
Analyzes alternative splicing at single-cell resolution. The first decision is library chemistry — 10X 3' is fundamentally limited (RT primes from poly-A, R2 falls in 3' UTR, <0.1 junction read per cell per AS event). Plate-based full-length methods (Smart-seq3, FLASH-seq, VASA-seq, STORM-seq) and single-cell long-read (MAS-Iso-seq, scISOr-Seq2) are the chemistries that give per-cell isoform structure. Tools include MARVEL (R, Smart-seq integrated), BRIE2 (Bayesian PSI with regulatory features and ELBO_gain test), scQuint (junction-cluster, plate-based; not for 10X), SpliZ (annotation-free Z-score), Psix (graph-smoothness regulated AS), and Sierra (alternative polyadenylation, often confused with AS). Use when analyzing isoform usage in scRNA-seq, identifying cell-type-specific splicing, or determining whether scRNA-seq chemistry supports splicing analysis at all.
日本語の概要は準備中です。原文の説明を表示しています。
Select, document, download, and checksum-verify the most appropriate NCBI reference genome on an HPC cluster. Use whenever an analysis needs a reference genome, genome FASTA, genome annotation, assembly accession, or genome index and no exact approved assembly is already available; when a user asks to fetch a species genome from NCBI; or when a pipeline must resolve and cache a reproducible reference assembly on a remote cluster.
日本語の概要は準備中です。原文の説明を表示しています。
Map nucleosome center positions, occupancy, and fuzziness from ATAC-seq fragment-size patterns using NucleoATAC, ATACseqQC, DANPOS3, or scprinter. Use when characterizing nucleosome organization at promoters and enhancers, calling +1/-1 nucleosomes flanking NFRs, generating V-plots for chromatin structure visualization, or comparing nucleosome positioning between conditions.
日本語の概要は準備中です。原文の説明を表示しています。
Predict enhancer-gene regulatory connections from ATAC-seq using ABC, ENCODE-rE2G, HiChIP, or Cicero. Use when linking distal enhancers to target genes, choosing between contact-aware (ABC, ENCODE-rE2G), accessibility-only (Cicero), and orthogonal (HiChIP H3K27ac, EpiMap) approaches, validating predictions against CRISPRi-FlowFISH gold-standard, or building cell-type-specific regulatory maps for fine-mapping or therapeutic target discovery.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about CMV oilfield operations — the 24-hour restart exemption (49 CFR 395.1(d)), oilfield-specific HOS rules, hot oil hauling, specialty equipment (winch trucks, kill trucks, frac sand haulers, water haulers), Texas Railroad Commission permitting, oilfield insurance specifics, and how oilfield operations differ from standard OTR trucking. Cite 49 CFR 395.1(d).
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks how to build + manage direct shipper relationships — moving from spot market to dedicated lanes, customer scoring + credit assessment, contract terms, dedicated capacity agreements, KPI reporting, and how to handle customer disputes. Reference standard customer-carrier contract terms.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about towing + recovery operations — separate FMCSA authority class, light-duty vs medium-duty vs heavy-duty wrecker rules, AAA + AAA-Plus contracts, Roadside-Assistance (RA) operations, "non-consensual tow" rules + state-by-state, and how towing operations differ from general freight. Reference state Public Utility Commission + 49 CFR 395 application.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about state-specific trucking taxes BEYOND IFTA — NY Highway Use Tax (HUT), KY Kentucky Intrastate Tax (KIT), Oregon Weight-Mile Tax, New Mexico Weight-Distance Tax, Connecticut Highway Use Fee, or other state-level road-use taxes for commercial vehicles. Covers thresholds, rates, registration, filing cadence, and how these differ from IFTA. Reference state DOT/DOR resources.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about passenger-carrying CMVs — buses, motorcoaches, charter operations, school buses, limousines, shuttle services. Covers different HOS rules (10-hour driving / 15-hour on-duty / no 34-restart), Class C CDL with P endorsement, S endorsement for school bus, FMCSA Hours-of-Service for passenger vehicles, $5M insurance minimum, BMC-32 filing, accessibility requirements, and how passenger ops differ from property. Cite 49 CFR 395.5 + 387.33.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when selecting or replacing an ELD provider. Covers the FMCSA Registered ELD list, evaluation criteria, common provider removals from the registered list, and the 60-day transition rule.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill to evaluate whether to switch from annual MVR pulls to continuous monitoring. Covers cost-benefit, intervention timing, and major providers.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill to understand the International Registration Plan (IRP). Covers when you need to register apportioned, how it differs from IFTA, and quarterly fees.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about Household Goods (HHG) movers — 49 CFR 375 + 376 consumer protection rules, FMCSA HHG-specific authority, mover certification, binding vs non-binding estimates, dispute resolution, weight ticket requirements, valuation coverage, and how HHG operations differ from general freight. Cite 49 CFR 375.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when re-hiring a driver who has been away from CMV operation for 1+ years. Covers re-qualification, refresh of medical and MVR, and whether prior employer inquiries restart.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about driver discipline — when to coach vs warn vs suspend vs terminate, progressive discipline frameworks, at-will employment in trucking, documenting violations, separating "willful violation" from "human error," and how to handle a driver who refuses corrective action. Reference state employment law + ATA SMP.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about carrier-driver relationships — Independent Contractor (IC / 1099) vs Employee (W-2), Owner-Operator lease agreements, the AB5 California law, the DOL 2024 IC test, lease-purchase programs from the carrier side, settlement statements, deductions, and how to structure a compliant IC arrangement. Cite 49 CFR 376 + 1099 IRS rules.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill to track recent and upcoming SMS methodology changes from FMCSA. Covers proposed rule changes, comment periods, and implementation timelines.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about driver fitness-for-duty programs — pre-trip readiness assessment, recognizing impairment from drugs/alcohol/medication/fatigue/mental health, refusing assignments when unfit, supervisor training on fitness assessment, FMCSA recommendations on duty status determination, and how to handle a driver who appears unfit. Reference 49 CFR 392.3 + 392.4.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when hiring a driver who has a recent valid pre-employment test from a prior employer. Covers the conditions under § 382.301(c) that allow accepting it instead of testing again.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about US-Canada cross-border CMV operations — CVOR (Commercial Vehicle Operator's Registration), NSC (National Safety Code), Ontario / Quebec / BC specific rules, US carrier obtaining Canadian operating authority, FAST card for drivers, customs (CBSA), Health Canada hazmat, and how Transport Canada equivalents differ from US FMCSA. Reference USMCA + Transport Canada NSC 14.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks how to investigate a CMV accident — what to do at the scene, the first 24 hours, evidence preservation, who to call, preventability determination, ELD data preservation, dash cam review, witness interviews, working with insurance + attorney, and how to build a learning culture from incidents. Reference 49 CFR 392.7 + insurance industry best practices.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about accident claims management — first-notice-of-loss procedures, working with insurance adjusters, claim severity classification, subrogation, claim closure timelines, and how to influence claim outcomes. Different from accident-investigation-playbook (which covers facts); this covers the insurance + claim side.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill to obtain a Motor Carrier (MC) number from FMCSA. Covers application, fees, and timeline.
日本語の概要は準備中です。原文の説明を表示しています。