その他のスキル

Sort alignment files by coordinate or read name using samtools and pysam. Use when preparing BAM files for indexing, variant calling, or paired-end analysis.

日本語の概要は準備中です。原文の説明を表示しています。

Generate consensus sequences and manage reference files using samtools. Use when creating consensus from alignments, indexing references, or creating sequence dictionaries.

日本語の概要は準備中です。原文の説明を表示しています。

View, convert, and understand SAM/BAM/CRAM alignment files using samtools and pysam. Use when inspecting alignments, converting between formats, or understanding alignment file structure.

日本語の概要は準備中です。原文の説明を表示しています。

Trim multiple sequence alignments using ClipKIT, trimAl, BMGE, Divvier, or HMMcleaner with mode selection guidance per downstream goal. Use when removing unreliable columns or contaminating residues before phylogenetic inference, HMM building, or selection analysis.

日本語の概要は準備中です。原文の説明を表示しています。

Align protein structures using Foldseek 3Di, TM-align, US-align, DALI, or Foldmason for structural MSA. Predict, score, and superpose backbone coordinates when sequence identity is below the twilight zone or remote-homology detection is required. Use when sequence MSA fails (<25% identity), when the dark proteome is the target, when AlphaFoldDB / ESM Atlas search is needed, or when structural superposition is the goal.

日本語の概要は準備中です。原文の説明を表示しています。

Creates sashimi-style plots showing RNA-seq read coverage and splice junction counts using ggsashimi (general-purpose, condition-grouped overlays), rmats2sashimiplot (rMATS-output-aware), MAJIQ-VOILA (LSV posteriors interactive HTML), leafviz (leafcutter clusters Shiny), Jutils (tool-agnostic heatmaps and sashimi for rMATS/leafcutter/SUPPA2/MAJIQ output), or pyGenomeTracks (multi-track publication figures). Tool choice depends on the upstream differential-splicing tool's output format and the publication vs interactive use case. Use when visualizing specific splicing events, validating differential splicing calls, or producing publication-quality figures.

日本語の概要は準備中です。原文の説明を表示しています。

Quantifies alternative splicing as PSI (percent spliced in) from RNA-seq using rMATS-turbo (BAM-based event), SUPPA2 (TPM-based event), MAJIQ V3 (LSV-based Bayesian), leafcutter (annotation-free intron clusters), VAST-TOOLS (cross-species with microexon support), Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage), or IRFinder-S (intron retention coverage-aware). Distinguishes the five canonical event classes (SE, A5SS, A3SS, MXE, RI), special classes (microexons, exitrons, AFE/ALE), intron retention subtypes (canonical RI vs detained introns), and applies effective-length normalization. Use when measuring splice-site usage or isoform inclusion ratios from short-read RNA-seq.

日本語の概要は準備中です。原文の説明を表示しています。

Detect allele-specific chromatin accessibility from ATAC-seq using WASP, GATK ASEReadCounter, or RASQUAL. Use when mapping cis-regulatory genetic variants from heterozygous SNPs, separating cis from trans regulation, building chromatin QTL (caQTL) maps, validating GWAS variant function with allelic imbalance, or detecting reference allele mapping bias before downstream analysis.

日本語の概要は準備中です。原文の説明を表示しています。

Infer cis-regulatory connections (peak-to-peak co-accessibility) from scATAC-seq using Cicero, ArchR getCoAccessibility, or SCENIC+. Use when linking enhancer accessibility to promoter accessibility, identifying enhancer-gene pairs from chromatin alone (without paired RNA), running gene-regulatory inference combining ATAC + RNA, or comparing predicted regulatory contacts against Hi-C/Micro-C ground truth.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks about the financial differences between being an owner-operator vs company driver — gross pay, expenses, net pay, capital requirements, risk tolerance, lifestyle, and how to evaluate the path. Reference industry pay surveys.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill to understand FMCSA tire tread depth requirements. Covers steer tires (4/32 minimum) and other axles (2/32 minimum), and how to measure correctly.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill for record retention after a driver leaves your employment. Covers when records can be destroyed vs retained for cause.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill to understand defensive driving course expectations for CMV drivers. Covers federal vs state vs carrier-specific requirements.

日本語の概要は準備中です。原文の説明を表示しています。

Use when a carrier is evaluating ELD / telematics / dashcam platforms and needs an objective comparison of Samsara, Motive (formerly KeepTruckin), and Geotab. Covers ELD compliance, AI dashcams, driver scoring, integrations, pricing structure, and which fleet profile suits which platform.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when documenting supervisor reasonable-suspicion training for Part 382 compliance. Covers what to log, what to retain, and what training providers to use.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when selecting a TPR-registered Training Provider for behind-the-wheel ELDT training. Covers the registry, evaluation criteria, and common pitfalls.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks about passenger-carrying CMVs — buses, motorcoaches, charter operations, school buses, limousines, shuttle services. Covers different HOS rules (10-hour driving / 15-hour on-duty / no 34-restart), Class C CDL with P endorsement, S endorsement for school bus, FMCSA Hours-of-Service for passenger vehicles, $5M insurance minimum, BMC-32 filing, accessibility requirements, and how passenger ops differ from property. Cite 49 CFR 395.5 + 387.33.

日本語の概要は準備中です。原文の説明を表示しています。

Use when a carrier or driver asks about winter trucking, snow chain requirements, hot-weather precautions, summer tire failures, fuel additive use (anti-gel), idle management in extreme temperatures, weight restrictions in spring thaw, or how seasonal demand patterns affect freight rates and capacity.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when buying a used tractor or trailer. Covers verifying compliance status, hidden issues, and what to inspect before signing.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill to understand trailer-specific inspection requirements when a trailer is not coupled to a tractor. Covers annual inspection, DVIR rules, and parking inspections.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill for training drivers on effective pre-trip inspection technique. Covers the comprehensive walk-around, time investment, and common areas drivers miss.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks the cost of pulling a Motor Vehicle Record (MVR) in a specific state, or wants to compare MVR fees across multiple states, or is budgeting annual compliance costs for a multi-state fleet. Reference the canonical fee table below; flag that fees change (re-verify with state DMV before annual budget planning).

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks about trucking insurance — types of coverage (commercial auto, cargo, occupational accident, workers comp, bobtail/non-trucking, MCS-90 endorsement), federal minimums (49 CFR 387), what each covers, owner-operator vs company-driver implications, and how to read a Certificate of Insurance (COI). Cite 49 CFR 387.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks about refrigerated (reefer) trucking — cold chain compliance, FDA temperature recording requirements, Sanitary Transportation Rule (STR / 21 CFR 1.900-1.934), commodity-specific temperature ranges, refrigeration unit operation, temperature recorder calibration, and how a load is challenged if temperature deviates. Cite 21 CFR Part 1 Subpart Q.

日本語の概要は準備中です。原文の説明を表示しています。