Other skills

Select, document, download, and checksum-verify the most appropriate NCBI reference genome on an HPC cluster. Use whenever an analysis needs a reference genome, genome FASTA, genome annotation, assembly accession, or genome index and no exact approved assembly is already available; when a user asks to fetch a species genome from NCBI; or when a pipeline must resolve and cache a reproducible reference assembly on a remote cluster.

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NCPGR高性能计算集群软件使用注意事项与优化指南。聚焦各软件的坑点、加速技巧、资源节省建议与软件选型推荐。当用户询问集群上如何高效使用软件、编写LSF作业、或需要软件选型建议时使用。

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Use this skill when the user asks about state-specific trucking taxes BEYOND IFTA — NY Highway Use Tax (HUT), KY Kentucky Intrastate Tax (KIT), Oregon Weight-Mile Tax, New Mexico Weight-Distance Tax, Connecticut Highway Use Fee, or other state-level road-use taxes for commercial vehicles. Covers thresholds, rates, registration, filing cadence, and how these differ from IFTA. Reference state DOT/DOR resources.

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Use this skill to understand defensive driving course expectations for CMV drivers. Covers federal vs state vs carrier-specific requirements.

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Use when a carrier is evaluating ELD / telematics / dashcam platforms and needs an objective comparison of Samsara, Motive (formerly KeepTruckin), and Geotab. Covers ELD compliance, AI dashcams, driver scoring, integrations, pricing structure, and which fleet profile suits which platform.

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Use this skill to evaluate document retention software options. Covers compliance features, ease of use, integration, and cost.

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Use this skill when the user asks about the financial differences between being an owner-operator vs company driver — gross pay, expenses, net pay, capital requirements, risk tolerance, lifestyle, and how to evaluate the path. Reference industry pay surveys.

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Use this skill when the user asks about CMV vehicle inspections under 49 CFR 396 — DVIRs (Driver Vehicle Inspection Reports), pre-trip inspections, post-trip inspections, periodic inspections, annual inspections, who can perform inspections, OOS criteria, and what makes a "no defect" DVIR valid. Cite 49 CFR 396.

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Use this skill for wheel-end maintenance and lubrication. Covers oil-bath vs grease packs, wheel-end loss issues, and inspection intervals.

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Use when an owner-operator (1099 or LLC/S-corp) asks how to lower their tax bill, what they can deduct, per diem treatment, depreciation choices (Section 179, bonus, MACRS), quarterly estimated payments, S-corp salary vs distributions, retirement plan options (SEP-IRA, Solo 401(k)), or recordkeeping requirements. Covers federal income tax + self-employment tax basics; not state-specific.

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Use this skill when the user asks about oversize / overweight load permits — state-by-state permitting process, escort vehicle (pilot car) requirements, route surveys, federal axle weight limits (Federal Bridge Formula), holiday restrictions, multi-state permits, and how to determine if a load needs a permit. Reference state DOT permitting offices.

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Use this skill when buying a used tractor or trailer. Covers verifying compliance status, hidden issues, and what to inspect before signing.

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Use this skill to understand trailer-specific inspection requirements when a trailer is not coupled to a tractor. Covers annual inspection, DVIR rules, and parking inspections.

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Use this skill for vehicle brake system inspection requirements. Covers daily DVIR, weekly maintenance, brake adjustment check, and the 'percent of out-of-adjustment' rule.

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Use this skill to understand record retention requirements for currently-employed drivers. Covers DQF, HOS, vehicle records, and D&A specific periods.

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Use when a carrier asks about trailer types — dry van, reefer, flatbed, step-deck, conestoga, tanker, dump, lowboy, intermodal chassis, auto-hauler. Covers length / weight rules, common specs, reefer unit options (Carrier vs Thermo King), liftgate options, kingpin setting, and trailer-to-tractor matching.

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Use when a carrier asks how to spec a new tractor — engine choice (X15 vs DD16 vs PACCAR MX), transmission (manual vs automated manual vs automatic), axle ratio, sleeper size, fuel tanks, aerodynamic options, electronic features, warranty, fleet-spec vs premium-spec tradeoffs, and how spec choices affect resale value.

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Use this skill when the user asks the cost of pulling a Motor Vehicle Record (MVR) in a specific state, or wants to compare MVR fees across multiple states, or is budgeting annual compliance costs for a multi-state fleet. Reference the canonical fee table below; flag that fees change (re-verify with state DMV before annual budget planning).

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Use this skill when the user asks about CMV oilfield operations — the 24-hour restart exemption (49 CFR 395.1(d)), oilfield-specific HOS rules, hot oil hauling, specialty equipment (winch trucks, kill trucks, frac sand haulers, water haulers), Texas Railroad Commission permitting, oilfield insurance specifics, and how oilfield operations differ from standard OTR trucking. Cite 49 CFR 395.1(d).

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Use when a carrier or driver asks about winter trucking, snow chain requirements, hot-weather precautions, summer tire failures, fuel additive use (anti-gel), idle management in extreme temperatures, weight restrictions in spring thaw, or how seasonal demand patterns affect freight rates and capacity.

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Use this skill when the user asks about trucking insurance — types of coverage (commercial auto, cargo, occupational accident, workers comp, bobtail/non-trucking, MCS-90 endorsement), federal minimums (49 CFR 387), what each covers, owner-operator vs company-driver implications, and how to read a Certificate of Insurance (COI). Cite 49 CFR 387.

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Use this skill when the user asks about refrigerated (reefer) trucking — cold chain compliance, FDA temperature recording requirements, Sanitary Transportation Rule (STR / 21 CFR 1.900-1.934), commodity-specific temperature ranges, refrigeration unit operation, temperature recorder calibration, and how a load is challenged if temperature deviates. Cite 21 CFR Part 1 Subpart Q.

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Use this skill when the user asks about team driving — two-driver OTR operations, how teams maximize miles, HOS rules with sleeper berth swap, pay structures, equipment requirements (sleeper berth specs), team retention, and common challenges. Reference 49 CFR 395.1(g).

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Use this skill when the user asks about passenger-carrying CMVs — buses, motorcoaches, charter operations, school buses, limousines, shuttle services. Covers different HOS rules (10-hour driving / 15-hour on-duty / no 34-restart), Class C CDL with P endorsement, S endorsement for school bus, FMCSA Hours-of-Service for passenger vehicles, $5M insurance minimum, BMC-32 filing, accessibility requirements, and how passenger ops differ from property. Cite 49 CFR 395.5 + 387.33.

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