4.9万 件 ・ 関連度順
概要と使いどころ
Parse and analyze multiple sequence alignments using Biopython. Extract sequences, identify conserved regions, analyze gaps, work with annotations, and manipulate alignment data for downstream analysis. Use when parsing or manipulating multiple sequence alignments.
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Analyzes differential transcript usage (DTU) and isoform switches with functional consequence prediction (NMD via 50nt rule, ORF disruption, protein domain loss/gain, signal peptide changes, IDR alterations, coding-potential shifts). Tools include IsoformSwitchAnalyzeR v2 (auto-selects satuRn for >5 reps else DEXSeq), the manual DRIMSeq -> DEXSeq/satuRn -> stageR DTU pipeline, and fishpond/swish for inferential-uncertainty-aware DTE. Distinguishes DTU from DGE and DTE; integrates external annotators (CPC2, Pfam, SignalP, IUPred2A or DeepTMHMM). Use when investigating how splicing differences alter protein function or trigger NMD-mediated degradation.
日本語の概要は準備中です。原文の説明を表示しています。
Detects differential alternative splicing between conditions using rMATS-turbo (binomial LRT on junction counts), leafcutter (Dirichlet-multinomial GLM on intron clusters), MAJIQ V3 deltapsi/HET (Bayesian posterior on LSVs), SUPPA2 (empirical-null on TPM-derived PSI), or Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage). Reports FDR-corrected significance and delta PSI effect sizes. Tools differ in statistical model, annotation dependence, calibration regime, and replicate-count requirements. Use when comparing splicing patterns between treatment groups, tissues, or disease states.
日本語の概要は準備中です。原文の説明を表示しています。
Perform pairwise sequence alignment using Biopython Bio.Align.PairwiseAligner. Use when comparing two sequences, finding optimal alignments, scoring similarity, and identifying local or global matches between DNA, RNA, or protein sequences.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about passenger-carrying CMVs — buses, motorcoaches, charter operations, school buses, limousines, shuttle services. Covers different HOS rules (10-hour driving / 15-hour on-duty / no 34-restart), Class C CDL with P endorsement, S endorsement for school bus, FMCSA Hours-of-Service for passenger vehicles, $5M insurance minimum, BMC-32 filing, accessibility requirements, and how passenger ops differ from property. Cite 49 CFR 395.5 + 387.33.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when documenting supervisor reasonable-suspicion training for Part 382 compliance. Covers what to log, what to retain, and what training providers to use.
日本語の概要は準備中です。原文の説明を表示しています。
Use when a carrier or driver asks about winter trucking, snow chain requirements, hot-weather precautions, summer tire failures, fuel additive use (anti-gel), idle management in extreme temperatures, weight restrictions in spring thaw, or how seasonal demand patterns affect freight rates and capacity.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when buying a used tractor or trailer. Covers verifying compliance status, hidden issues, and what to inspect before signing.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks how to build + manage direct shipper relationships — moving from spot market to dedicated lanes, customer scoring + credit assessment, contract terms, dedicated capacity agreements, KPI reporting, and how to handle customer disputes. Reference standard customer-carrier contract terms.
日本語の概要は準備中です。原文の説明を表示しています。
Use when a carrier asks about trailer types — dry van, reefer, flatbed, step-deck, conestoga, tanker, dump, lowboy, intermodal chassis, auto-hauler. Covers length / weight rules, common specs, reefer unit options (Carrier vs Thermo King), liftgate options, kingpin setting, and trailer-to-tractor matching.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about CMV oilfield operations — the 24-hour restart exemption (49 CFR 395.1(d)), oilfield-specific HOS rules, hot oil hauling, specialty equipment (winch trucks, kill trucks, frac sand haulers, water haulers), Texas Railroad Commission permitting, oilfield insurance specifics, and how oilfield operations differ from standard OTR trucking. Cite 49 CFR 395.1(d).
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill for record retention after a driver leaves your employment. Covers when records can be destroyed vs retained for cause.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill to develop the capability to investigate crashes internally. Covers what to look for, when to escalate to outside experts, and documentation standards.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about oversize / overweight load permits — state-by-state permitting process, escort vehicle (pilot car) requirements, route surveys, federal axle weight limits (Federal Bridge Formula), holiday restrictions, multi-state permits, and how to determine if a load needs a permit. Reference state DOT permitting offices.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about team driving — two-driver OTR operations, how teams maximize miles, HOS rules with sleeper berth swap, pay structures, equipment requirements (sleeper berth specs), team retention, and common challenges. Reference 49 CFR 395.1(g).
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about truck purchase financing, leasing options, factoring, fuel surcharge math, lease-purchase agreements, the total-cost-of-ownership (TCO) comparison between buying + leasing, and how owner-operators finance their first truck. Cover Section 179 + Bonus Depreciation tax treatment.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill to understand trailer-specific inspection requirements when a trailer is not coupled to a tractor. Covers annual inspection, DVIR rules, and parking inspections.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks how to design or improve a Preventive Maintenance (PM) program for a fleet — mileage vs time intervals, A/B/C/D inspection levels, ATA standards, common PM tasks, scheduling software, cost tracking, and how PM affects roadside inspections + CSA scores. Reference ATA Equipment Maintenance Council standards.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks the cost of pulling a Motor Vehicle Record (MVR) in a specific state, or wants to compare MVR fees across multiple states, or is budgeting annual compliance costs for a multi-state fleet. Reference the canonical fee table below; flag that fees change (re-verify with state DMV before annual budget planning).
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about CMV roadside inspection levels (Level I through Level VIII), what each level inspects, average inspection times, CVSA decal eligibility, what triggers a level upgrade, and how to coach drivers through a roadside inspection. Reference CVSA North American Standard Inspection Program.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when selecting a TPR-registered Training Provider for behind-the-wheel ELDT training. Covers the registry, evaluation criteria, and common pitfalls.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about state-specific trucking taxes BEYOND IFTA — NY Highway Use Tax (HUT), KY Kentucky Intrastate Tax (KIT), Oregon Weight-Mile Tax, New Mexico Weight-Distance Tax, Connecticut Highway Use Fee, or other state-level road-use taxes for commercial vehicles. Covers thresholds, rates, registration, filing cadence, and how these differ from IFTA. Reference state DOT/DOR resources.
日本語の概要は準備中です。原文の説明を表示しています。
Use when a carrier asks how to spec a new tractor — engine choice (X15 vs DD16 vs PACCAR MX), transmission (manual vs automated manual vs automatic), axle ratio, sleeper size, fuel tanks, aerodynamic options, electronic features, warranty, fleet-spec vs premium-spec tradeoffs, and how spec choices affect resale value.
日本語の概要は準備中です。原文の説明を表示しています。
Use this skill when the user asks about getting/maintaining motor carrier operating authority — USDOT number, MC number, BOC-3 process agent, insurance filing (MCS-90, BMC-91/91X), Unified Carrier Registration (UCR), International Registration Plan (IRP), International Fuel Tax Agreement (IFTA), state-level permits (NY HUT, KY weight distance, OR weight-mile, NM weight distance), new entrant safety audit, intra vs interstate, for-hire vs private. Cite 49 CFR 365, 387, 390.
日本語の概要は準備中です。原文の説明を表示しています。